All chemical solvents were purchased from Thermo Fisher Scientific (Waltham, MA). Substrates diclofenac sodium, flufenamic acid (flufenamate), meclofenamic acid (meclofenamate), mefenamic acid (mefenamate), and tolfenamic acid (tolfenamate) were purchased from MilliporeSigma (Burlington, MA). Substrates aceclofenac and lumiracoxib were purchased from Abcam Inc. (Cambridge, MA). Trapping agent dansyl glutathione trifluoroacetic acid salt was purchased from Toronto Research Chemicals (Toronto, ON, Canada). Internal standard dansylamide and reducing agent Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) were obtained from MilliporeSigma. Human liver microsomes pooled from 150 donors (HLM150) and recombinant Supersomes containing cytochromes CYP2C8, 2C9, 2C19, and 3A4 were obtained from Corning Gentest (Woburn, MA). Marvin 20.4 was used for drawing, displaying, and characterizing chemical structures, substructures, and reactions (ChemAxon, http://www.chemaxon.com ).
Dansylamide
Manufactured by Merck Group
Sourced in Canada
Dansylamide is a fluorescent labeling reagent commonly used in analytical and biochemical applications. It functions as a fluorescent tag that can be attached to various molecules, enabling their detection and quantification.
Automatically generated - may contain errors
Lab products found in correlation
Dansyl glutathione trifluoroacetic acid salt, by LGC (4 mentions)
Meclofenamic acid, by Merck Group (3 mentions)
Aceclofenac, by Abcam (2 mentions)
Lumiracoxib, by Abcam (2 mentions)
Diclofenac sodium, by Merck Group (2 mentions)
Flufenamic acid, by Merck Group (2 mentions)
Tris 2 carboxyethyl phosphine hydrochloride, by Merck Group (2 mentions)
Tris 2 carboxyethyl phosphine hydrochloride tcep, by Merck Group (2 mentions)
Hlm150, by Corning (2 mentions)
Glucose 6 phosphate (g6p), by Merck Group (2 mentions)
Nadp disodium salt, by Merck Group (2 mentions)
Glucose 6 phosphate dehydrogenase, by Merck Group (2 mentions)
Magnesium chloride salt, by Thermo Fisher Scientific (1 mentions)
Dansylcadaverine, by Merck Group (1 mentions)
4 protocols using dansylamide
1
Characterization of NSAID Metabolism
2
Hepatic Metabolism of Selective BET Inhibitors
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Chemical solvents were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Substrate I-BET151 was purchased from Cayman Chemical (Ann Arbor, MI, USA) and substrates OXFBD02 and OXFBD04 were synthesized as reported previously [28 (link),31 (link)]. Trapping agent dansyl glutathione trifluoroacetic acid salt was purchased from Toronto Research Chemicals (Toronto, ON, Canada) while internal standard dansylamide was obtained from Millipore Sigma. Reducing agent tris(2-carboxyethyl)phosphine hydrochloride (TCEP) was purchased from Millipore Sigma. Human liver microsomes pooled from 150 donors (HLM150) were purchased from Corning Gentest (Woburn, MA, USA).
3
In Vitro Characterization of NSAID Metabolism
4
Quantitative Profiling of Hepatic Biotransformation
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All chemical solvents were purchased from Thermo Fisher Scientific (Waltham, MA). The following chemicals were purchased from Millipore-Sigma (Burlington, MA): substrate meclofenamic acid (meclofenamate); internal standard dansylamide; reducing agent Tris(2-carboxyethyl)phosphine hydrochloride; NADPH-regenerating system components NADP disodium salt, glucose-6-phosphate dehydrogenase, and glucose-6-phosphate, as well as dansyl cadaverine and dansyl amidoethylmercaptan. Magnesium chloride salt was purchased from Thermo Fisher Scientific. Trapping agent dansyl glutathione trifluoroacetic acid salt was purchased from Toronto Research Chemicals (Toronto, ON, Canada). Human liver microsomes pooled from 150 donors [human liver microsomes 150 (HLM150)] were purchased from Corning Gentest (Woburn, MA). Marvin 20.4 was used for drawing, displaying, and characterizing chemical structures, substructures, and reactions (http://www.chemaxon.com ; ChemAxon).
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