AICAR and metformin were purchased from Toronto Research Chemicals (ON, Canada) and Sigma-Aldrich (MO, USA) respectively. Compound C and JNK-IN-8 were from Millipore (MA, USA) and Selleck (China) respectively. palmitate/BSA solution was prepared as previously described 22 (link). In brief, 100 mM palmitate (Sigma-Aldrich) stock was dissolved in 5% (w/v) fatty acid free BSA (PAA Laboratories, QLD, Australia) in a 55℃ water bath and filtered. The palmitate and BSA solutions were cooled to room temperature and diluted in serum-free medium to concentrations of 0.25 mM and 0.25%, respectively.
Fatty acid free bsa
Manufactured by GE Healthcare
Sourced in Australia, Austria
Fatty acid free bovine serum albumin (BSA) is a highly purified protein preparation used as a stabilizer and carrier in various biomedical applications. It serves as a protective agent, blocking non-specific binding, and provides a protein-rich environment for cell culture and biochemical assays.
Automatically generated - may contain errors
Lab products found in correlation
Palmitate, by Merck Group (2 mentions)
Metformin, by LGC (1 mentions)
Aicar, by LGC (1 mentions)
Jnk in 8, by Merck Group (1 mentions)
Compound c, by Merck Group (1 mentions)
Rgfp966, by Selleck Chemicals (1 mentions)
Lysotracker red dnd 99, by Thermo Fisher Scientific (1 mentions)
Hoechst 33342, by Thermo Fisher Scientific (1 mentions)
Apoa 1, by Merck Group (1 mentions)
Rg2833, by Selleck Chemicals (1 mentions)
Filipin 3 from streptomyces filipinensis, by Merck Group (1 mentions)
Lysosensor yellow blue dnd 160, by Thermo Fisher Scientific (1 mentions)
N n dimethylformamide (dmf), by Merck Group (1 mentions)
Bafilomycin a1, by Merck Group (1 mentions)
1 2 3h n cholesterol, by PerkinElmer (1 mentions)
3 protocols using fatty acid free bsa
1
Palmitate-induced Metabolic Stress Protocol
2
Reagents for Niemann-Pick Type C Studies
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RG2833 and RGFP966 were purchased from Selleck Chemicals LLC (Houston, TX, USA). U18666A and GPN were obtained from Cayman Chemical Company (Ann Arbor, MI, USA). LysoTracker Red DND-99, LysoSensor Yellow/Blue DND-160 and Hoechst 33342 were from Molecular Probes/Invitrogen (Thermo Fisher Scientific, Darmstadt, Germany). GFP-certified FluoForte was from Enzo Life Sciences GmbH (Lörrach, Germany). Filipin III from streptomyces filipinensis, N,N-dimethylformamide and ApoA-I were purchased from Sigma-Aldrich Chemie GmbH (Taufkirchen, Germany). Bafilomycin A1 was obtained either from Calbiochem/Merck Millipore (Darmstadt, Germany) or from Tocris Bioscience (Bristol, UK). Fatty acid-free BSA was from PAA Laboratories (Pasching, Austria). [1,2-3H(N)]Cholesterol (1.813 TBq/mmol) was from PerkinElmer LAS (Rodgau, Germany). All other chemicals were from previously described sources18 (link)20 (link). The mouse NPC1-His6-EYFP expression plasmid (NPC1-YFP; Addgene plasmid 53523) was kindly provided by Dr. Matthew P. Scott via Addgene69 (link).
3
Palmitate/BSA Solution Preparation and Treatment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
palmitate/BSA solution was prepared as previously described 26 (link). Briefly, palmitate (Sigma-Aldrich) was dissolved in 0.1mM NaOH at 70℃ and diluted 1:20 in 5% (w/v) fatty acid free BSA (PAA Laboratories) in PBS to make a 5mM palmitate/ 5% BSA solution. The solution was then incubated in 56℃ water bath for 10 min, cooled to room temperature and filtered. Then, the palmitate/BSA solution was diluted in DMEM to final concentration of 0.75mM palmitate/ 0.75% BSA or 0.5mM palmitate/ 0.75% BSA. Differentiated C2C12 myotubes in 24-well plates was then incubated with above solutions for 24h with 10 nM insulin stimulation for the last 30 min.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!