Three human GBC cell lines (NOZ, GBC-SD, and SGC-996) and the human intrahepatic biliary epithelialcell line HIBEC used in the present studywere purchased from Cell Bank of theChinese Academy of Science (Shanghai, China). Cells were cultured in DMEM (Gibco, Invitrogen, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, Invitrogen, US, lot: 10099-141c) and 0.5% penicillin/streptomycin (Gibco, Invitrogen, USA). Cells were maintained at 37 °C in a humidi ed atmosphere containing 5% CO 2 .
Hibec
Manufactured by National Collection of Authenticated Cell Cultures
Sourced in China
HIBEC is a laboratory instrument designed for the cultivation and maintenance of human and animal cell cultures. It provides a controlled environment with precise temperature, humidity, and CO2 regulation to support the optimal growth and proliferation of cells. The core function of HIBEC is to create and maintain the optimal conditions required for cell culture experiments and research.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Gbc sd, by National Collection of Authenticated Cell Cultures (3 mentions)
Sgc 996, by National Collection of Authenticated Cell Cultures (3 mentions)
Rpmi 1640 complete medium, by Sartorius (2 mentions)
Hucct1, by National Collection of Authenticated Cell Cultures (2 mentions)
Cclp 1, by National Collection of Authenticated Cell Cultures (2 mentions)
Hccc9810, by National Collection of Authenticated Cell Cultures (1 mentions)
5 protocols using hibec
1
Culturing GBC and HIBEC Cell Lines
2
Culturing GBC and HIBEC Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Three human GBC cell lines (NOZ, GBC-SD, and SGC-996) and the human intrahepatic biliary epithelialcell line HIBEC used in the present studywere purchased from Cell Bank of theChinese Academy of Science (Shanghai, China). Cells were cultured in DMEM (Gibco, Invitrogen, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, Invitrogen, US, lot: 10099-141c) and 0.5% penicillin/streptomycin (Gibco, Invitrogen, USA). Cells were maintained at 37 °C in a humidi ed atmosphere containing 5% CO 2 .
3
Culturing GBC and Normal Biliary Cells
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Three human GBC cell lines (NOZ, GBC-SD, and SGC-996) and the normal human intrahepatic biliary epithelial cell line HIBEC used in the present study were purchased from Cell Bank of the Chinese Academy of Science (Shanghai, China). Cells were cultured in DMEM (Gibco, Invitrogen, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, Invitrogen, US) and 0.5% penicillin/streptomycin (Gibco, Invitrogen, USA). Cells were maintained at 37 °C in a humidified atmosphere containing 5% CO2.
4
Cultivation of Intrahepatic Biliary Cell Lines
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Four ICC cell lines (CCLP-1, HCCC-9810, RBE and HuCCT-1) and a normal human intrahepatic biliary epithelial cell line (HIBEC) were purchased from Cell Bank of Type Culture Collection of Chinese Academy of Sciences, (Shanghai, China). Cells were cultivated according to the protocols from their supplier. All cell lines were grown in RPMI-1640 complete medium (Biological Industries, Kibbutz Beit-Haemek, Israel) supplemented with 10% fetal bovine serum (FBS; Moregate Biotech, Brisbane, Australia), and were cultured in an incubator of 37°C and 5% CO2.
5
Cultivation of Intrahepatic Biliary Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Three ICC cell lines (CCLP-1, RBE and HuCCT-1) and a normal human intrahepatic biliary epithelial cell line (HIBEC) were purchased from Cell Bank of Type Culture Collection of Chinese Academy of Sciences, (Shanghai, China). Cells were cultivated according to the protocols from their supplier. All cell lines were grown in RPMI-1640 complete medium (Biological Industries, Kibbutz Beit-Haemek, Israel) supplemented with 10% fetal bovine serum (FBS; Moregate Biotech, Brisbane, Australia), and were cultured in an incubator of 37°C and 5% CO2.
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