Hob c

The human osteosarcoma cell lines, U-2OS (origin from a female 15y old osteosarcoma patient), SaOS, MNNG/HOS, and MG63 were purchased from the American Type Culture Collection (Rockville, MD) in 2014. The human osteosarcoma KHOS (origin from a female 13y old osteosarcoma patient) cell line was provided by Dr. Efstathios Gonos (Institute of Biological Research & Biotechnology, Athens, Greece) in 2008. KHOS and U-2OS are two commonly used cell lines in osteosarcoma research. The human osteoblast cell lines NHOst and HOB-c were purchased from LonzaWalkersville (Walkersville, MD) and PromoCell GmbH (Heidelberg, Germany) respectively in 2013. The osteosarcoma cell lines were cultured in RPMI-1640 (Life Technologies, Grand Island, NY) supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 mg/mL streptomycin (Life Technologies, Carlsbad, CA). The osteoblast cell lines were cultured in osteoblast growth medium (PomoCell) with 10% fetal bovine serum. All cells were maintained in a humidified atmosphere containing 5% CO₂ at 37 °C. Bicalutamide (Sigma-Aldrich, St. Louis, MO) is a synthetic, non-steroidal AR antagonist and a pure antiandrogen used in the treatment of prostate cancer, hirsutism, and other androgen-dependent conditions. Doxorubicin was purchased from APP pharmaceuticals, LLC, (Lake Zurich, Illinois).

The human osteoblast cells NHOst and HOB-c were purchased from Lonza Walkersville (Walkersville, MD) and PromoCell GmbH (Heidelberg, Germany), respectively. The human osteosarcoma cell lines, U-2OS, SaOS, MG63, MNNG/HOS, and 143B were obtained from the American Type Culture Collection (Rockville, MD). The human osteosarcoma KHOS cell line was kindly provided by Dr. Efstathios Gonos (Institute of Biological Research & Biotechnology, Athens, Greece). The osteosarcoma cell lines were cultured in RPMI-1640 (Life Technologies, Grand Island, NY) supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 mg/mL streptomycin (Life Technologies, Carlsbad, CA). The osteoblast cell lines were cultured in osteoblast growth medium (PromoCell) with 10% fetal bovine serum. All cells were maintained in a humidified atmosphere containing 5% CO₂, 95% air atmosphere at 37°C.