The probes were perfused at 2 μL/min with artificial cerebrospinal fluid (Zhang et al., 2006; Vazquez et al., 2008) . Two hours after the reflux, the dialysate sample was collected in 10-min fractions. Three samples were obtained to establish the baseline levels of extracellular dopamine (DA) before drug administration and 12 samples were obtained thereafter. DA levels were measured using a HTEC-500 HPLC system with an-electro chemical detection system (Eicom Co., Ltd.) with a C18 reversed phase column (4.6 mmφx 30 mm, Eicompak PP-ODS; Eicom Co., Ltd.) (Kaizaki et al., 2014) .
Htec 500 hplc system
Manufactured by Eicom
The HTEC-500 HPLC system is a high-performance liquid chromatography instrument designed for laboratory use. It is capable of performing liquid chromatography separations and analyses. The HTEC-500 HPLC system includes a solvent delivery system, a sample injection system, a chromatographic column, and a detector.
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Lab products found in correlation
2 protocols using htec 500 hplc system
1
Microdialysis Sampling of Extracellular Dopamine
2
HPLC Detection of Neurotransmitter Levels
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Neurotransmitter concentrations were detected using Eicom HTEC 500 HPLC system. The standard solutions of NE (MW 337.3), 5-HT (MW 212.68) and Isoproterenol (internal standard; MW 247.7) were each 1 ng/μL concentrations. Sample preparations were carried out as previously described (Agarwal et al., 2011 (link); Wilson et al., 2014a (link)).
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