Cf h260azi

Manufactured by Olympus

Sourced in Japan

The CF-H260AZI is a high-resolution confocal microscope designed for advanced imaging applications. It features a compact design, high-sensitivity detectors, and a motorized stage for precise sample positioning. The CF-H260AZI is capable of capturing detailed images of various samples, though its specific intended use is not provided.

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34 protocols using cf h260azi

Colonoscopy Equipment Evaluation and Treatment

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In the retroflexion ability analysis, the CF-H260AZI, PCF-Q260AZI, or CF-HQ290I (Olympus) was used in the latest evaluating colonoscopy. PCF-Y0062 is the prototype of the novel PCF-H290TI colonoscope. As our institution had only one, it was impossible to use it for all cases during the study period, considering that there were so many treatments being performed simultaneously, and the scope required adequate cleaning time. Therefore, the PCF-Y0062 was preferentially used on lesions for which some difficulties were anticipated, such as poor endoscope operability identified during the evaluating colonoscopy. A conventional gastroscope, GIF-Q260 J, GIF-H260Z, or GIF-H260 (Olympus), was used for some lesions located in the sigmoid colon and rectum that required retroflexion, and a conventional colonoscope, PCF-Q260AZI, or CF-H260AZI (Olympus), was used for the other lesions.
In the treatment outcome analysis, the conventional colonoscopes included the PCF-Y0021 (prototype, Olympus), PCF-Y0047 (prototype, Olympus), PCF-Q260AZI, CF-H260AZI, and PCF-Q260JI (Olympus).

Tanaka H., Oka S., Tanaka S., Inagaki K., Okamoto Y., Matsumoto K., Boda K., Yamashita K., Sumimoto K., Ninomiya Y., Hayashi N, & Chayama K. (2019). The utility of a novel colonoscope with retroflexion for colorectal endoscopic submucosal dissection. Endoscopy International Open, 7(2), E130-E137.

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Colorectal ESD with Poor Maneuverability

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Patients treated between April 2011 and April 2018 at our hospital were retrospectively reviewed. Maneuverability and indication were confirmed on preoperative endoscopy (CF‐HQ290ZI or CF‐H260AZI; Olympus, Tokyo, Japan), with magnifying function, in 400 consecutive cases of colorectal ESD performed during the study period. Poor maneuverability comprised paradoxical movement of the endoscope,10 poor control with adhesion after abdominal surgery, and redundant colon. Of the 125 cases judged as having poor maneuverability, 36 cases of sigmoid colon without the use of BAE, 2 cases of incomplete total colonoscopy, and 4 cases with the use of a single balloon overtube for the small intestine (ST‐SB 1; Olympus) were excluded. Finally, 83 deep colon cases judged as having poor maneuverability were included. Among these, 54 cases underwent BAE, introduced in March 2015 at our hospital (group B), and 29 cases underwent conventional procedures before BAE was introduced (group C) (Fig. 1). This study was approved by our Institutional Review Board (No. F2018C12); all patients were provided with the opportunity to opt out of the study.

Kuroki Y., Asonuma K., Uehara N., Endo T., Suzuki R., Yamamoto Y, & Nagahama M. (2019). Efficacy and suitable indication of colorectal endoscopic submucosal dissection using a balloon‐assisted endoscope. JGH Open: An Open Access Journal of Gastroenterology and Hepatology, 4(2), 185-190.

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Endoscopic Resection Techniques for Colorectal Neoplasms

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Indications of EMR for CRNs followed the JGES guidelines for colorectal ESD/EMR [18 (link)]. ER is recommended for CRNs ≥ 6 mm in size and superficial depressed-type CRNs ≤ 5 mm in size.
EMR was performed by multiple endoscopists with more than 1000 conventional endoscopic experiences using a high-resolution video colonoscope (CF-H260AZI, PCF-Q260AZI, CF-HQ290ZI, or PCF-H290ZI (Olympus, Tokyo, Japan)) or a gastroscope (GIF-Q260J (Olympus)) for rectal lesions. We used a 10% glycerin solution containing a small amount of indigo carmine (indigo carmine/glycerol: 2 mL/200 mL) as the injection solution. A spiral snare, SnareMaster (Olympus), or Captivator II Snare (Boston Scientific, Boston, MA, USA) was used; selection was dependent on the tumor size or the particular situation. At the end of the procedure, hemostatic clips were rarely used, and hemostatic forceps were used when post-EMR hemostasis was necessary. All exposed vessels on the resected ulcer were coagulated using hemostatic forceps (Coagrasper (Olympus) or HDB2418W-W (Pentax, Tokyo, Japan)) in conjunction with a high-frequency generator (ESG-100 or ESG-300 (Olympus)). We performed a blood examination in all patients the day after EMR. If the blood examination and abdominal findings were within normal range and regular, the patient was permitted to eat a meal and leave the hospital the same day.

Inagaki K., Yamashita K., Oka S., Tanino F., Yamamoto N., Kamigaichi Y., Tamari H., Shimohara Y., Nishimura T., Okamoto Y., Tanaka H., Kotachi T., Yuge R., Urabe Y., Kitadai Y., Yoshimura K, & Tanaka S. (2021). Risk of Bleeding after Colorectal Endoscopic Resection in Patients with Continued Warfarin Use Compared to Heparin Replacement: A Propensity Score Matching Analysis. Gastroenterology Research and Practice, 2021, 9415387.

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Detailed Colonoscopy Procedure with Magnification

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Prior to the examination, patients underwent a bowel preparation with 2–3 liters of a polyethylene glycol solution. Diazepam and butyl scopolamine were used intravenously for sedation and prevention of peristalsis. All patients underwent total colonoscopies with magnifying endoscopes (CF-240ZI, CF-H260AZI, PCF-240ZI; Olympus Corp., Tokyo, Japan) using approximately 80- to 100-fold magnification. During the magnifying observation, on-site endoscopists first sprayed the target lesion with indigo carmine, and if necessary, they then stained the lesion with crystal violet. All detected lesions were diagnosed on the basis of the PIT classification system described by Kudo et al (14 (link)). Each diagnosis was recorded in the database just after the colonoscopy. Lesion size, location and shape [Paris classification 10 (21 (link))] were also measured and recorded. The lesions diagnosed as non-neoplastic were left untreated. Conversely, all neoplastic lesions, except for type IIIL PIT DAPs, were completely removed. Type IIIL PIT DAPs were left untreated at the discretion of the on-site endoscopists as Fig. 2.

Maeda Y., Kudo S.E., Wakamura K., Miyachi H., Misawa M., Mori Y., Ogata N., Kudo T., Kodama K., Hisayuki T., Hayashi T., Katagiri A, & Ishida F. (2017). The concept of ‘Semi-clean colon’ using the pit pattern classification system has the potential to be acceptable in combination with a <3-year surveillance colonoscopy. Oncology Letters, 14(3), 2735-2742.

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Evaluating Peyer's Patches in Terminal Ileum

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Colonoscopic examinations were performed using a magnifying video colonoscope system (CF-H260AZI™; Olympus, Tokyo, Japan) with a video processor system (EVIS 260 Lucera Spectrum™; Olympus), while PPs in the terminal ileum, within 10 cm from Bauhin's valve, were observed using NBI-ME. In accordance with previous literature, we defined PPs as regions comprising more than five domes.10 (link) When several PPs were observed in the terminal ileum, we evaluated the PP that was located closest to the anus.

Hiyama S., Iijima H., Kawai S., Mukai A., Shiraishi E., Iwatani S., Yamaguchi T., Araki M., Hayashi Y., Shinzaki S., Mizushima T., Tsujii M, & Takehara T. (2016). Narrow-band imaging with magnifying endoscopy for Peyer's patches is useful in predicting the recurrence of remissive patients with ulcerative colitis. Intestinal Research, 14(4), 314-321.

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Magnifying Colonoscopy for Colorectal Lesion Diagnosis

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Upon detection of a lesion by standard colonoscopy, the surface mucus was washed away with lukewarm water and indigo carmine dye was spread over the lesion. When it was not possible to adequately stain the surface with indigo carmine for diagnosis, crystal violet dye was used instead, and magnifying observation was performed. All images were obtained with magnifying colonoscopies (CF-Q240ZI, CF-H260AZI, and CF-H290ZI; Olympus, Tokyo, Japan) with up to 80-fold magnification in combination with a standard video processor system (EVIS LUCERA system, EVIS EXERA system; Olympus Inc., Tokyo, Japan). Pit pattern diagnosis was based on the dominant pit pattern according to the Kudo and Tsuruta classification [13 (link), 14 (link)] as well as the dominant narrow-band imaging (NBI) findings as proposed by the Japan NBI Expert Team (JNET) classification [15 (link), 16 ]. The JNET classification divides vessel and surface patterns into 4 categories: types 1, 2A, 2B, and 3, which are consistent with the histopathological findings of hyperplastic polyp/sessile serrated polyp, low-grade intramucosal neoplasia, high-grade intramucosal neoplasia/shallow submucosal invasive cancer, and deep submucosal invasive cancer, respectively.

Hirano D., Oka S., Tanaka S., Sumimoto K., Ninomiya Y., Tamaru Y., Shigita K., Hayashi N., Urabe Y., Kitadai Y., Shimamoto F., Arihiro K, & Chayama K. (2017). Clinicopathologic and endoscopic features of early-stage colorectal serrated adenocarcinoma. BMC Gastroenterology, 17, 158.

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High-Resolution Colonoscopy for Polyp Evaluation

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All procedures were performed using high-resolution magnifying colonoscopes (CF-H260AZI, CF-H290ZI, or PCF-H290ZI colonoscope, EVIS LUCERA ELITE System; Olympus, Tokyo, Japan). Polyp location, size, and morphology[15 ] were recorded by physicians in both groups. Locations were categorized as being on the right side of the colon (cecum, ascending, and transverse colon); on the left side of the colon (descending and sigmoid colon); or within the rectum, and were classified based on the Japanese Classiﬁcation of Colorectal Carcinoma[16 ]. Pathological evaluations were performed according to the Vienna classiﬁcation and the classiﬁcations of the Japanese Society for Cancer of the Colon and Rectum[16 ].

Sugimoto S., Yabana T., Nomura T., Hayashi S., Okuda N., Temma T., Hashimoto Y., Ito T., Takami M., Oyamada J, & Kamei A. (2020). Can Non-expert Physicians Use the Japan Narrow-band Imaging Expert Team Classification to Diagnose Colonic Polyps Effectively?. Journal of the Anus, Rectum and Colon, 4(3), 100-107.

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Colorectal Tumor Characterization via Endoscopy

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Colorectal tumors were observed at high magni-fication using high-resolution magnifying endoscopes (CF-H260AZI; Olympus, Tokyo, Japan) after staining with indigo carmine dye and 0.05% crystal violet. Microsurface structures were classified according to Kudo's pit pattern classification system [44 (link), 45 (link)]. Most often, one biopsy specimen was collected from each lesion for extracting genomic DNA. When more than two subcomponents were found in a single lesion, biopsy specimens were obtained for each portion, as described previously [5 (link), 7 (link)]. The lesions were then treated through endoscopic mucosal resection, endoscopic submucosal dissection or surgical resection, after which histological analyses were carried out. Conventional adenomas were subcategorized into low-grade or high-grade adenomas. High-grade adenomas were defined as being 1 cm or more in diameter and/or with villous components and/or with high-grade dysplasia.

Aoki H., Yamamoto E., Takasawa A., Niinuma T., Yamano H.O., Harada T., Matsushita H.O., Yoshikawa K., Takagi R., Harada E., Tanaka Y., Yoshida Y., Aoyama T., Eizuka M., Yorozu A., Kitajima H., Kai M., Sawada N., Sugai T., Nakase H, & Suzuki H. (2017). Epigenetic silencing of SMOC1 in traditional serrated adenoma and colorectal cancer. Oncotarget, 9(4), 4707-4721.

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Detecting Dysplastic Lesions in Colonoscopy

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Possible flat-type dysplastic lesions were detected based on the color change using regular white-light colonoscopy (CF-Q240I, H290AI or PCF-Q260AI, Q260AZI, H290I; Olympus, Tokyo, Japan). Identified lesions were observed following 0.2–0.4 % indigo carmine dye spraying. To further determine whether these areas were dysplastic or non-neoplastic, subsequent magnifying colonoscopy (CF-H260AZI, HQ290I or PCF-Q260AZI, H290AZI; Olympus) using NBI and/or 0.05 % crystal violet staining were performed to identify their lines of demarcation. The endoscopic morphology was classified in accordance with the SCENIC international consensus statement. Briefly, flat lesions were defined as tumors without elevation or depression relative to the surrounding mucosa and could be detected only by their color change. Classifications were determined retrospectively after thorough discussion by two expert endoscopists (YI and MN) based on the detailed information provided by reporting endoscopists. The endoscopic disease activity was assessed by trained colonoscopists in accordance with the Mayo endoscopic score.

Ikebata A., Shimoda M., Okabayashi K., Uraoka T., Maehata T., Sugimoto S., Mutaguchi M., Naganuma M., Kameyama K., Yahagi N., Kanai T., Kitagawa Y., Kanai Y, & Iwao Y. (2021). Demarcated redness associated with increased vascular density/size: a useful marker of flat-type dysplasia in patients with ulcerative colitis. Endoscopy International Open, 9(4), E552-E561.

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Colorectal Polyp Pathological Diagnosis

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The diagnosis of colorectal polyps (adenomatous versus non-adenomatous hyperplastic) was based on the results of the pathological examinations. These images, including WLI, NBI, and CEI, were captured by a video endoscopy system (EVIS LUCERA ELITE system: Olympus Medical Systems, Co. Ltd, Tokyo, Japan) using colonoscopes (CF-H260AZI, CF-H260I, CF-260AI, and PCF-Q260AI: Olympus Medical Systems) during routine colonoscopic examination. These images were extracted from routine colonoscopies at Kindai University Hospital. Ethical permission for this study was granted by the review boards of Kindai University Hospital. Informed consent was not obtained because of the retrospective design of the study.

Komeda Y., Handa H., Matsui R., Hatori S., Yamamoto R., Sakurai T., Takenaka M., Hagiwara S., Nishida N., Kashida H., Watanabe T, & Kudo M. (2021). Artificial intelligence-based endoscopic diagnosis of colorectal polyps using residual networks. PLoS ONE, 16(6), e0253585.

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