Discontinuous native PAGE was performed as described elsewhere [44] (link). Two different incubation solutions (a and b) were used to visualize starch synthase activities. The incubation buffer (a) contained 50 mM Tricine/KOH (pH 8.0), 25 mM K-acetate, 5 mM DTE, 2 mM EDTA, 0.025% (w/v) bovine serum albumin, and 1 mM ADPglucose (either purchased from Sigma, Taufkirchen, Germany or synthesised using recombinant AGPase from E. coli as described in Fettke et al. [44] (link)). Buffer (a) was used for gels that contained 0.02% (w/v) glycogen from oyster (type II, Sigma). Gels without any glucan were incubated with buffer (b) that contained in addition 500 mM Na-citrate (pH 8.0). After electrophoresis the gels were equilibrated for 20 min in the respective buffer, but ADPglucose was omitted. Gels were incubated overnight at room temperature and stained with iodine.
Glycogen from oyster type 2
Manufactured by Merck Group
Sourced in United States
Glycogen from oyster (type II) is a high molecular weight polysaccharide that serves as a storage form of glucose in certain organisms, including oysters. It is classified as a type II glycogen, which refers to its specific structural and functional characteristics. This product is intended for use in research and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Adp glucose, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Apigenin, by Extrasynthese (1 mentions)
Flavone, by Merck Group (1 mentions)
Hesperidin, by Merck Group (1 mentions)
Chlorogenic acid, by MP Biomedicals (1 mentions)
Bovine albumin, by Merck Group (1 mentions)
Rutin, by Merck Group (1 mentions)
Heparin, by Merck Group (1 mentions)
Trolox, by Merck Group (1 mentions)
Luminol, by Merck Group (1 mentions)
Quercetin, by Merck Group (1 mentions)
Kaempferol, by Merck Group (1 mentions)
Hplc grade methanol, by Tedia (1 mentions)
2 protocols using glycogen from oyster type 2
1
Native PAGE for Starch Synthase Activity
2
Antioxidant and Anti-inflammatory Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Caffeic acid and 3-D-galactoside-quercetin were obtained from Fluka Chemie (Buchs, Switzerland). Hesperidin and rutin were purchased from Merck (Darmstadt, Germany). Apigenin was obtained from Extrasynthese (Genay Cedex France) and chlorogenic acid from MP Biomedicals (Illkirch, France). Mangiferin was obtained as a secondary standard metabolite from Mangifera indica L. Methanol (HPLC grade) was purchased from Tedia (Fairfield, USA). Trifluoroacetic acid (analytical grade) was obtained from Vetec (Rio de Janeiro, Brazil). Luminol (5-amino-2,3-dihydro-1, 4-phthalazinedione), quercetin, glycogen from oyster (type II), bovine albumin, heparin, kaempferol, flavone and LPS (Lipopolysaccharide from Escherichia coli) were all purchased from Sigma Chemicals (St. Louis, MO, USA). AAPH (2,2’-azobis-2-methyl-propanimidamide, dihydrochloride) and trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxilic acid) were purchased from Aldrich Chemical (Milwaukee, WI, USA). Glycine was purchased from Nuclear (Diadema, Brazil). All other chemicals were of reagent grade.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!