Immune-deficient nude (Nu/Nu) mice were obtained from Jackson Laboratory or Charles River and fed with standard chow diet. Cells were mixed with equal volume of Matrigel Matrix (Corning, #354230) and injected (RXF-393= 1 × 106, A498= 2 × 106 and OSRC-2= 1.5 × 106 cells/injection) subcutaneously in the flanks of 5–6 weeks-old nude (Nu/Nu) mice. Caliper measurements of growing tumors were taken periodically and the tumor volume was calculated. For glutaminase inhibition study, Caki-1 human clear cell renal cell carcinoma cells were mixed 1:1 with Matrigel and implanted subcutaneously in the flanks into female scid/bg mice (Charles River) (2.5 × 106 cells/mouse). On day 16 post-implant, mice were randomized to the following two groups (n=10 mice per group): 1) vehicle (25% hydroxyl-propyl-β-cyclodextrin) orally twice daily by gavage; or 2) CB-839 at 200 mg/kg orally twice daily by gavage. For all in vivo studies, tumors were measured with calipers and tumor volume was calculated using the formula = (a x b2/2) where ‘b’ is the smallest diameter and ‘a’ is the largest perpendicular diameter.
Female scid bg mice
Manufactured by Charles River Laboratories
Sourced in United States
Female scid/bg mice are a laboratory mouse model that have a severe combined immunodeficiency (scid) mutation and a beige (bg) coat color mutation. They are characterized by a lack of functional T and B cells, making them suitable for research involving xenografts or other applications requiring an immunocompromised host.
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Lab products found in correlation
2 protocols using female scid bg mice
1
Subcutaneous tumor xenograft models
2
In Vivo Bioluminescence Imaging of Adenoviral Transduction
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Female SCID-bg mice at 4–6 weeks of age were obtained from Charles River Laboratories (Wilmington, MA, USA). Before i.v. administration, Ad5-CMV-GFP-luc (1 × 108 ifu/mouse) was pre-incubated for 1 h at room temperature in the absence or presence of sCAR-CXCL12 (5 μg) in a volume of 100 μL. Three days following virus administration, mice were anesthetized with ketamine/xylazine, injected intraperitoneally with D-luciferin (300 μL; 125 mg/kg body weight), and scanned using an IVIS 100 imaging system (PerkinElmer, Waltham, MA, USA). Bioluminescence images were analyzed using Living Image software version 3.0 (PerkinElmer). Regions of interest (ROI) were drawn over the tumor and liver area, and total photon counts were calculated. Following imaging, mice were sacrificed, and livers were extracted, snap frozen, and stored at −80°C. The frozen sections were processed later for DNA extraction and determination of viral E4 copy number by real-time PCR.
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