Aspartate transaminase (AST), alanine transaminase (ALT), malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), and total proteins were sourced from Biodiagnostic Company, Giza, Egypt. Reverse transcriptase, DNA ladder (100 bp), Oligo dT primers, SYBR Green PCR Master Mix, and TRIzol were from Invitrogen. The kits to determine total antioxidant activity (cat # TA 2513) were from Biodiagnostic Co. ELISA kits for mouse IL‐1β (ab197742) and myeloperoxidase (ab155458) were from Abcam Co.
Alanine transaminase
Manufactured by Biodiagnostic
Alanine transaminase is an enzyme that catalyzes the transfer of an amino group from alanine to alpha-ketoglutarate, producing pyruvate and glutamate. It is present in various tissues, particularly the liver, and is commonly used as a biomarker for liver function.
Automatically generated - may contain errors
Lab products found in correlation
Aspartate transaminase, by Biodiagnostic (2 mentions)
Ab155458, by Abcam (1 mentions)
Reverse transcriptase, by Thermo Fisher Scientific (1 mentions)
Ab197742, by Abcam (1 mentions)
Oligo dt primer, by Thermo Fisher Scientific (1 mentions)
Sybr green pcr master mix, by Thermo Fisher Scientific (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Urea, by Biodiagnostic (1 mentions)
2 protocols using alanine transaminase
1
Oxidative Stress Biomarkers Protocol
2
Biochemical Assays for Liver and Kidney Functions
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Kits for the enzymes aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), creatinine, and urea were purchased from Biodiagnostic Co., Egypt. The transaminase activities were determined calorimetrically as a change in absorbance/minute at the wavelength 505 nm, as described by Reitman and Frankel (1957 (link)). Serum ALP activity was detected colorimetrically at the wavelength 510 nm according to Belfield and Goldberg (1971 (link)). Creatinine was detected according to Bartels et al. (1972 ) and urea levels were measured using the method of Fawcett and Scott (1960 (link)). Serum total protein kits were obtained from Spectrum Diagnostics., Al-Obour city, Cairo, Egypt, and determined by the colorimetric method at 546 nm wavelength according to Sapan et al. (1999 (link)).
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