Risperidone content was assayed using a Shimadzu Prominence HPLC system consisting of a degasser (Model DGU-20A5, Tokyo, Japan), a pump (Model LC-20AD, Tokyo, Japan), an autosampler (Model SIL-20AC, Tokyo, Japan), a UV-Vis detector (Model SPD-20A, Tokyo, Japan), and a column oven (Model CTO-20AC, Tokyo, Japan). Chromatographic analysis was performed with a C18 column (CNW Technologies Athena, 120 Å, 5 μm, 250 mm × 4.6 mm, Tokyo, Japan) at 25 °C. Methanol/H2O/triethylamine at a ratio of 80/19.5/0.5 v/v/v was used as mobile phase, while acetic acid was used to adjust the pH at 10.22. The flow rate was set at 1.0 mL/min, injection volume was 20 μL, while the risperidone analysis was performed at 254 nm.
Model sil 20ac
Manufactured by Shimadzu
Sourced in Japan
The Shimadzu SIL-20AC is an autosampler module designed for high-performance liquid chromatography (HPLC) systems. It is capable of automated sample injection and sample preparation. The SIL-20AC provides precise and reliable sample handling to ensure consistent and reproducible chromatographic results.
Automatically generated - may contain errors
Lab products found in correlation
Model spd 20a, by Shimadzu (5 mentions)
Model lc 20ad, by Shimadzu (5 mentions)
Model dgu 20a5, by Shimadzu (4 mentions)
Model cto 20ac, by Shimadzu (4 mentions)
Pack pro c18, by YMC (1 mentions)
Hplc system, by Shimadzu (1 mentions)
Cto 20ac, by Shimadzu (1 mentions)
Prominence hplc system, by Shimadzu (1 mentions)
Lc solution software, by Shimadzu (1 mentions)
Model spd m20a, by Shimadzu (1 mentions)
Model lc 20at, by Shimadzu (1 mentions)
C18 column, by Waters Corporation (1 mentions)
6 protocols using model sil 20ac
1
Risperidone Quantification by HPLC
2
HPLC Quantification of Galantamine
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Galantamine quantification was determined by using a Shimadzu Prominence HPLC system that consisted of a degasser (Model DGU-20A5, Tokyo, Japan), a pump (Model LC-20AD, Tokyo, Japan), an autosampler (Model SIL-20AC, Tokyo, Japan), a UV–Vis detector (Model SPD-20A, Tokyo, Japan), and a column oven (Model CTO-20AC, Tokyo, Japan). HPLC analysis was performed with a C18 column (CNW Technologies Athena, 120 Å, 5 μm, 250 mm × 4.6 mm, Tokyo, Japan) at 25 °C. A 10 mM aqueous solution of KH2PO4 with a pH of 3.5 was used as phase A, and methanol was used as phase B. The mobile phase consisted of A/B 80/20 v/v. The flow rate was set at 1.0 mL/min, the injection volume was 10 μL, and the galantamine analysis was performed at 235 nm.
3
Quantification of Taxifolin in LK-ME by HPLC
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The concentration of taxifolin in LK-ME was quantified using high performance liquid chromatography (HPLC). The HPLC system (Shimadzu Corporation, Kyoto, Japan) consist of a Model LC-20AD high pressure pump, a Model CTO-20AC column oven, a Model SIL-20AC total-volume injection-type auto-sampler, and a Model SPD-20A variable wavelength UV–Vis detector. Samples were separated using YMC-Pack Pro C18 (internal diameter: 3.0 mm, length: 150 mm, YMC, Kyoto, Japan) at 40 °C and the mobile phase consisted of 10 mM phosphoric acid (A) and acetonitrile (B) at 0.5 ml/min flow rates. Purified taxifolin (20 μg/ml) was used as the standard compound, and the concentration of taxifolin in LK-ME was calculated by the peak area of absorbance units at 280 nm compared with the standard. The HPLC analysis was performed by the Biodynamic Plant Institute, Sapporo, Hokkaido, Japan.
4
HPLC Quantification of Risperidone
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Risperidone content was assayed using a well-established HPLC method previously used in our lab [31 (link)]. In brief a Shimadzu Prominence HPLC system consisting of a degasser (Model DGU-20A5, Tokyo, Japan), a pump (Model LC-20AD, Tokyo, Japan), an autosampler (Model SIL-20AC, Tokyo, Japan), a UV-Vis detector (Model SPD-20A, Tokyo, Japan) and a column oven (Model CTO-20AC, Tokyo, Japan) was used. Chromatographic analysis was performed with a C18 column (CNW Technologies Athena, 120 Å, 5 μm, 250 mm × 4.6 mm, Tokyo, Japan) at 25 °C. Methanol/H2O/triethylamine at a ratio of 80/19.5/0.5 v/v/v was used as the mobile phase, while acetic acid was used to adjust the pH at 10.2. The flow rate was set at 1.0 mL/min, injection volume was 20 μL, while the Risperidone analysis was performed at 254 nm.
5
Aripiprazole Microspheres HPLC Analysis
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MPs’ yield, drug loading and encapsulation efficiency (EE) were determined by applying the following equations:
Microspheres equivalent to 10 mg of aripiprazole were dissolved in the minimum quantity of dichloromethane and then diluted with the mobile phase: H2O pH 3.5: acetonitrile 60:40 (v/v). The resulting solution was filtered through 0.45 μm filter paper and the filtrate was assayed for ARI using a Shimadzu Prominence HPLC system (Shimadzu Corporation, Kyoto, Japan), consisting of a degasser (Model DGU-20A5), a pump (Model LC-20AD), an automatic sampler (Model SIL-20AC), an ultraviolet–visible variable detector (Model SPD-20A) (λmax = 254 nm) and a thermostatic oven (Model CTO-20AC). A reverse phase C18 column (250 mm × 4.6 mm I.D., 5 µm particle size) was used for chromatographic analysis. The flow rate was adjusted to 1 mL/min and the infusion volume was 20 μL. The chromatograms obtained were processed with the LC Solution software (v1.2, Shimadzu Corporation, Kyoto, Japan). All measurements were conducted in triplicate.
Microspheres equivalent to 10 mg of aripiprazole were dissolved in the minimum quantity of dichloromethane and then diluted with the mobile phase: H2O pH 3.5: acetonitrile 60:40 (v/v). The resulting solution was filtered through 0.45 μm filter paper and the filtrate was assayed for ARI using a Shimadzu Prominence HPLC system (Shimadzu Corporation, Kyoto, Japan), consisting of a degasser (Model DGU-20A5), a pump (Model LC-20AD), an automatic sampler (Model SIL-20AC), an ultraviolet–visible variable detector (Model SPD-20A) (λmax = 254 nm) and a thermostatic oven (Model CTO-20AC). A reverse phase C18 column (250 mm × 4.6 mm I.D., 5 µm particle size) was used for chromatographic analysis. The flow rate was adjusted to 1 mL/min and the infusion volume was 20 μL. The chromatograms obtained were processed with the LC Solution software (v1.2, Shimadzu Corporation, Kyoto, Japan). All measurements were conducted in triplicate.
6
Quantitative Analysis of Sorafenib in Rat Plasma
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The rat plasma was analyzed with a Shimadzu
HPLC system (Model SIL-20AC), which was a combination of a liquid
chromatographic pump (Model LC-20AT), a chromatographic autosampler
(Model SIL-20AC), and a photo diode array detector (Model SPD-M20A,
Shimadzu, Kyoto, Japan). The rat plasma analytes were separated by
a C18 column (50 mm × 2.1 mm i.d.; particle size 1.7
μm, Waters Acquity, Dublin, Ireland) and a guard column. To
ensure accurate chromatographic analysis of sorafenib and the internal
standard, the configured sorafenib stoke solution was diluted to different
concentrations of the working solution for the experiment. The mobile
phase was composed of 10 mM KH2PO4 (pH 3.0 adjusted
by phosphoric acid) and acetonitrile (55:45, v/v). The total running
time was 10 min, and the flow rate was 0.2 mL/min. The pump pressure
was controlled under 8535 psi. The temperature of the autosampler
and column oven were maintained at 10 and 25 °C, respectively.
The sample injection volume was set at 5 μL, and the peak integration
of the UV wavelength was set at 265 nm.
HPLC system (Model SIL-20AC), which was a combination of a liquid
chromatographic pump (Model LC-20AT), a chromatographic autosampler
(Model SIL-20AC), and a photo diode array detector (Model SPD-M20A,
Shimadzu, Kyoto, Japan). The rat plasma analytes were separated by
a C18 column (50 mm × 2.1 mm i.d.; particle size 1.7
μm, Waters Acquity, Dublin, Ireland) and a guard column. To
ensure accurate chromatographic analysis of sorafenib and the internal
standard, the configured sorafenib stoke solution was diluted to different
concentrations of the working solution for the experiment. The mobile
phase was composed of 10 mM KH2PO4 (pH 3.0 adjusted
by phosphoric acid) and acetonitrile (55:45, v/v). The total running
time was 10 min, and the flow rate was 0.2 mL/min. The pump pressure
was controlled under 8535 psi. The temperature of the autosampler
and column oven were maintained at 10 and 25 °C, respectively.
The sample injection volume was set at 5 μL, and the peak integration
of the UV wavelength was set at 265 nm.
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