A total of 1 × 105 cells were seeded into 12-well plates and cultured with DMEM without FBS. After 48 h, the cell culture media were collected to analyze TGF-β1 level by using a Human TGF-β1 ELISA kit (Proteintech) in accordance with the manufacturer’s protocols.
Human tgf β1 elisa kit
Manufactured by Proteintech
Sourced in United States
The Human TGF-β1 ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human transforming growth factor beta 1 (TGF-β1) levels in biological samples.
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5 protocols using human tgf β1 elisa kit
1
TGF-β1 Quantification by ELISA
2
TGF-β1 Release from GM-5000 Incubation
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GM-5000TGF-β1 (2 mg) were incubated in 9.57 mM phosphate-buffered saline solution (PBS, pH 7.4). At each point, the buffer was removed and replaced with fresh PBS. After 24 hr, PBS was replaced with collagenase. TGF-β1 concentrations released from GM were measured using human TGF-β1 ELISA kit (Proteintech Inc., Rosemont, USA).
3
TGFβ1 Secretion in Pancreatic Cancer Cells
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AsPC-1/vector, AsPC-1/TIPE2 cells, Panc02/vector and Panc02/TIPE2 cells were seeded in 6-well plate at a destiny of 2 × 105 and 1 × 105. The supernatants were collected after 48 h of incubation and the protein level of TGFβ1 was analyzed using human TGFβ1 ELISA kit (Proteintech) and mouse TGFβ1 ELISA kit (Biosensis, Thebarton, South Australia).
4
Quantifying NFκB and TGFβ1 in Cells
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To evaluate NFκB p65, lymphocytes (5 × 10 4 cells/well) were lysed, and total and phosphorylated (Ser536) NFκB p65 were measured using a NFκB p65 (Total/Phospho) Human InstantOne™ ELISA Kit (Thermo Fisher Scientific), according to the manufacturer's protocol.
To evaluate TGFβ1, tumor cells were seeded into a 6-well plate. After 48 h of incubation, supernatants were collected, and the concentration of TGFβ1 was measured using the Human TGFβ1 ELISA Kit (Proteintech, USA) according to the manufacturer's protocol.
To evaluate TGFβ1, tumor cells were seeded into a 6-well plate. After 48 h of incubation, supernatants were collected, and the concentration of TGFβ1 was measured using the Human TGFβ1 ELISA Kit (Proteintech, USA) according to the manufacturer's protocol.
5
Measuring TGF-β1 Levels in Tumor Cells
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Supernatants from UPP1-overexpressing tumor cells and control cells were analyzed using the human TGF-β1 ELISA kit (Proteintech, KE00002), per the manufacturer’s instructions. The TGF-β1 levels were detected using the SpectraMax ABS Plus microplate reader (MD, USA).
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