Human serum albumin solution was purchased from Baxter (Vienna, Austria). Vitamin C (AA) injection was supplied by Shenya Animal Healthcare (Shanghai, China). Mouse monoclonal antibodies to heme oxygenase 1 (HO-1, #MA1-112) and mouse monoclonal antibodies to L-ferritin (#MA5-14733) were purchased from Thermo Fisher Scientific (Rockford, United States). Rabbit monoclonal antibody to ß-Actin (#AC026) was purchased from Abclonal (Wuhan, China). Rabbit monoclonal antibody to nuclear factor erythroid 2-related factor 2 (Nrf2, # ab92946), rabbit monoclonal antibodies 4-hydroxy-2-nonenal (4-HNE, #ab46545), goat anti-mouse IgG H&L (HRP) (#ab6789), goat anti-rabbit IgG H&L (HRP) (#ab6721), a catalase (CAT) activity assay kit (#ab83464), a glutathione peroxidase (GPx) assay kit (#ab102530), and an 8-hydroxy 2 deoxyguanosine (8-OHdG) assay kit (#ab201734) were purchased from Abcam (Cambridge, MA, United States). A total antioxidant capacity (T-AOC) assay kit (#A015-2-1) and superoxide dismutase (SOD) kit (#A001-3-1) were obtained from Jiancheng Biotech. (Nanjing, China). A malondialdehyde (MDA) kit (#S0131S) was supplied by Beyotime Biotechnology (Shanghai, China). An enhanced chemiluminescence (ECL) kit (#PF001) was a product from Affinity Biosciences (Changzhou, China).
Enhanced chemiluminescence ecl kit
Manufactured by Affinity Biosciences
Sourced in China
The Enhanced chemiluminescence (ECL) kit is a laboratory reagent designed to detect and quantify proteins in Western blot analysis. The kit utilizes a chemiluminescent substrate to produce a light signal proportional to the amount of target protein present in the sample. This signal can be captured and analyzed using specialized imaging equipment.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti rabbit igg h l hrp, by Abcam (1 mentions)
Malondialdehyde mda kit, by Beyotime (1 mentions)
Superoxide dismutase sod kit, by Nanjing Jiancheng (1 mentions)
Goat anti mouse igg h l hrp, by Abcam (1 mentions)
Glutathione peroxidase gpx assay kit, by Abcam (1 mentions)
Atf6α, by Santa Cruz Biotechnology (1 mentions)
Grp78, by Santa Cruz Biotechnology (1 mentions)
Interleukin 4 (il 4), by Santa Cruz Biotechnology (1 mentions)
Il 13, by Santa Cruz Biotechnology (1 mentions)
Tissuelyser, by Jingxin (1 mentions)
2 protocols using enhanced chemiluminescence ecl kit
1
Antioxidant Biomarker Assessment Protocol
2
Western Blot Analysis of Lung Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The lung tissue was cut up on ice with RIPA buffer lysate and then homogenized by tissuelyser (JINGXIN, Shanghai, China) to extract the total protein. Equal amounts of lung homogenate or cell lysate were separated by a 12% SDS-PAGE and then transferred to PVDF membrane. The PVDF membrane was blocked in 5% non-fat milk for 1 hr. The primary antibodies were as follow: IL-4, IL-5, IL-13, CHOP, XBP1, ATF6α and GRP78 (Santa Cruz, CA). These antibodies were incubated at room temperature for 3 h and then overnight at 4°C. The next morning, after washing with TBST for three times, the corresponding secondary antibody was incubated for 1 h and then washed with TBST for three times. Finally, Enhanced Chemiluminescence (ECL) Kit (Affinity, China) and Chemiluminescence imaging system (Qin Xiang, Shanghai, China) were used to observe the protein immunostaining.
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