Potassium hydroxide

The pH cycling protocol consisted of using artificial neutral remineralizing saliva
(calcium 1.54 mmol / L; phosphate 1.54 mmol / L; acetic acid 20 mmol / L, and 0.308 g
of ammonium acetate, pH adjusted to 7.0 with potassium hydroxide (Vetec, Rio de
Janeiro, RJ, Brazil), and demineralizing saliva (3 mmol / L of calcium; 3 mmol /L of
phosphate; 50 mL acetic acid / L; ammonium acetate and 0.308 g with pH adjusted to
4.5 with sodium hydroxide (Vetec, Rio de Janeiro, RJ, Brazil).
To induce a strong cariogenic challenge, test specimens were stored in demineralizing
saliva for 22 consecutive hours. After being washed with deionized water, they were
kept in contact with remineralizing saliva for two hours in order to complete the
24-hour cycle. During the pH cycling period, the specimens were kept in an incubator
(Fanem Ltda, São Paulo, SP, Brazil), at a constant temperature of 37 °C to simulate
the oral environment. This dynamics was reproduced for a period of 28 days, during
which the artificial saliva (neutral and acid) was changed every two days.11 (link)

Bullfrog oil was provided by Asmarana Produtos Naturais (Natal, Brazil). Sodium hydroxide (NaOH), dimethyl sulfoxide (DMSO), potassium hydroxide, diethyl ether, hydrochloric acid (HCl), Tween^® 20, sodium thiosulfate, and sodium carbonate were from VETEC (Rio de Janeiro, Brazil). Miglyol^® 812 was a gift from Sasol (Witen, Germany). Butylhyldroxytoluene (BHT), buthylhydroxyanisole, potassium biphthalate, potassium dichromate, and Wijs solution were purchased from Labsynth (São Paulo, Brazil). Acetic acid, ethanol P.A, sodium bicarbonate, potassium iodide, starch, and chloroform were from Isofar (Rio de Janeiro, Brazil). Sucralose, Tutti-frutti flavoring and Acesulfame K were purchased from Valdequímica (São Paulo, Brazil). Xanthan gum, sodium benzoate and propylparaben were from ViaFarma (São Paulo, Brazil). Phenolphthalein was provided from Biotec Chemicals (Londrina, Brazil). Mucin Type II (Mucin from porcine stomach) and Span^® 80 were from Sigma-Aldrich (São Paulo, Brazil).