Horseradish peroxidase hrp conjugated goat anti rabbit igg

The following primary Abs were used: Rabbit anti‐Fam70a Ab (Cat#: bs‐11006R; Bioss); Rabbit anti‐Wnt5a Ab (Cat#: A12744; ABclonal); Rabbit anti‐adenomatous polyposis coli (APC) Ab (Cat#: AF0547; Affinity Biosciences); Rabbit anti‐Phospho Catenin Beta (Ser33/37/Thr41) Ab (Cat#: DF2989; Affinity Biosciences); Rabbit anti‐Phospho Akt (Thr308) Ab (Cat#: 13038; Cell Signaling Technology); Rabbit anti‐Phospho Akt (Ser473) Ab (Cat#: A5030; Bimake.Com); Mouse anti‐β‐actin Ab (Cat#: A5316‐100); Mouse anti‐β‐tubulin Ab (Cat#: sc‐5274); Mouse anti‐GAPDH Ab (Cat#: 30201ES60; YEASEN); Rabbit anti‐Beta‐Catenin Ab (Cat#: A5038; Bimake.Com); Mouse anti‐Strep II Tag Ab (Cat#: YFMA0054; YI FEI XUE BIOTECHNOLOGY); Mouse anti‐Flag Tag Ab (Cat#: YFMA0036; YI FEI XUE BIOTECHNOLOGY).
The following secondary Abs were used: TRITC‐conjugated Donkey anti‐Mouse IgG (Code: 715‐025‐150) and Cy2‐conjugated Donkey anti‐Rabbit IgG (Code: 711‐225‐152) were purchased from Jackson ImmunoResearch Laboratory. Horseradish peroxidase (HRP)‐conjugated goat anti‐rabbit IgG and HRP‐conjugated goat anti‐mouse IgG were purchased from Vazyme. Donkey Anti‐rabbit IgG/Gold (35 nm, Cat#: bs‐0295D‐Gold) and Donkey Anti‐rabbit IgG/Gold (15 nm, Cat#: bs‐0295D‐Gold) were purchased from Bioss.

All chemicals and reagents were obtained from Sigma unless otherwise stated.
Primary antibodies were mouse anti‐β‐actin (Cat#: A5316‐100); mouse anti‐GAPDH (Cat#: 30201ES60; YEASEN); mouse polyclonal a anti‐α‐Tubulin (cat#:G3115); mouse polyclonal a anti‐β‐Tubulin (cat#:sc‐5274); mouse monoclonal anti‐alpha tubulin (Acetyl Lys40) (cat#:bsm‐33235M; Bioss); rabbit polyclonal anti‐mphosh6 (cat#:10695‐1‐AP; Proteintech); rabbit polyclonal anti‐BubR1 (cat#:ab28193; Abcam); human anti‐centromere CREST (Cat#: 15‐234; Antibodies Incorporated); mouse polyclonal anti‐cyclin B1 (Cat#: B8566); rabbit polyclonal anti‐Phosphorylation CDK1 (Thr14) (Cat#: OAAN02724; Aviva Systems Biology); and rabbit anti‐Phosphorylation AKT (s473) (Cat#: 9271; Cell Signaling Technology). Dilution ratio for each primary antibody is in Table S1.
Fluorescent labelled secondary antibodies were all purchased from Jackson ImmunoResearch. The stock conc. was 1.5 mg/mL and used at 1:750 dilution: Cy2‐conjugated donkey anti‐mouse IgG (Code: 715‐225‐150); Cy2‐conjugated donkey anti‐rabbit IgG (Code: 711‐225‐152); Cy5‐conjugated donkey anti‐mouse IgG (Code: I715‐175‐150); and Rhodamine (TRITC)‐conjugated donkey anti‐rabbit IgG (Code: 711‐025‐152). Horseradish peroxidase (HRP)‐conjugated goat anti‐rabbit IgG and goat anti‐mouse IgG secondary antibodies were all purchased from Vazyme and used at 1:5000 dilution.

Mouse monoclonal anti-β-actin (Cat#: A5316-100) was purchased from Santa Cruz (St. Louis, MO, USA). Rabbit polyclonal anti-trim75 (Cat#: sc-249091) was purchased from Santa Cruz (Dallas, Texas, USA). Mouse monoclonal anti-β-tubulin antibody (Cat#: sc-5274) antibody was purchased from Santa Cruz (Dallas, Texas, USA). Human anti-centromere CREST antibody (Cat#:15–234) was purchased from Antibodies Incorporated (Davis, CA, USA). Cy2-conjugated donkey anti-mouse IgG (Code:715-225-150), rhodamine(TRITC)-conjugated donkey anti-goat IgG (Code:705-025-147), and 647-conjugated donkey anti-Human IgG (Code:709-605-149) were purchased from Jackson ImmunoResearch Laboratory (West Grove, PA, USA). Horseradish Peroxidase (HRP)-conjugated rabbit anti goat IgG and HRP-conjugated goat anti mouse IgG were purchased from Vazyme (Nanjing, jiangsu, China).