Os 30

Manufactured by Opti-Sciences

Sourced in United States, United Kingdom

The OS-30 is a compact, portable chlorophyll content meter designed for non-destructive measurement of leaf chlorophyll levels. It utilizes dual-wavelength optical technology to provide accurate and reliable chlorophyll readings.

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Lab products found in correlation

Imaging pam chlorophyll fluorometer, by Walz (1 mentions) Li 1600, by LI COR (1 mentions)

7 protocols using os 30

Chlorophyll Fluorescence Imaging Analysis

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Modulated chlorophyll fluorescence was measured in tagged and dark-adapted (30min) leaves, using a chlorophyll fluorometer OS-30 (OptiSciences, Herts, UK) with an excitation source intensity of 3000 μmol m^–2 s^–1. A special version of an Imaging-PAM Chlorophyll Fluorometer (Walz) was used to investigate spatio-temporal changes in photosynthetic parameters (Schreiber, 2004 ).

Albacete A., Cantero-Navarro E., Großkinsky D.K., Arias C.L., Balibrea M.E., Bru R., Fragner L., Ghanem M.E., González M.D., Hernández J.A., Martínez-Andújar C., van der Graaff E., Weckwerth W., Zellnig G., Pérez-Alfocea F, & Roitsch T. (2014). Ectopic overexpression of the cell wall invertase gene CIN1 leads to dehydration avoidance in tomato. Journal of Experimental Botany, 66(3), 863-878.

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Photosynthetic Efficiency Measurement Protocol

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Modulated chlorophyll fluorescence was measured in dark adapted (30 min) leaves in the second fully expanded leaf leaflet in 3 plants per graft combination (12 in total per contrasting group), using a chlorophyll fluorimeter OS-30 (OptiSciences, Herts, UK) with an excitation source intensity of 3000 mmol m⁻² s⁻¹. The minimal fluorescence intensity (F0) in a dark-adapted state was measured in the presence of a background far-red light. The maximal fluorescence intensity in the dark-adapted state (Fm) was measured by 0.8 s saturating pulses (3000 mmol m⁻² s⁻¹). The maximum quantum yield of open photosystem II (PSII) (Fv/Fm) was calculated as (Fm−F₀)/Fm (Maxwell and Johnson, 2000 (link)).

Martínez-Andújar C., Albacete A., Martínez-Pérez A., Pérez-Pérez J.M., Asins M.J, & Pérez-Alfocea F. (2016). Root-to-Shoot Hormonal Communication in Contrasting Rootstocks Suggests an Important Role for the Ethylene Precursor Aminocyclopropane-1-carboxylic Acid in Mediating Plant Growth under Low-Potassium Nutrition in Tomato. Frontiers in Plant Science, 7, 1782.

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Leaf Physiological Measurements in Plants

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Flag leaf area in cm² was measured using Area Meter (L1-Cor, Model L1 3000A.).
Specific leaf weight (SLW) in g cm⁻² was calculated according to Pearce et al. (1968) using the formula:

SLW = \frac{leaf d r y w e i g h t (g)}{l e a f a r e a ({c m}^{2})}

Relative water content (RWC %) was calculated based on Gonzalez and Gonzalez (2001) using the formula:

R W C = \frac{Fw - D w}{T w - D w}

where: FW = the sample fresh weight, TW = the turgid weight & DW = the dry weight.
Leaf chlorophyll fluorescence was measured using chlorophyll fluorometer (OS-30, opti sciences, inc. The USA) to calculate the maximum quantum yield of photo-system ll (PSll) by the formula of Maxwell and Johnson (2000) (link) as follow:

\frac{Fv}{Fm} = \frac{Fm - F o}{Fm}

where: Fv/Fm = the maximal quantum efficiency of PSll (MQE), Fm = the maximal chlorophyll fluorescence, and Fo = minimum chlorophyll fluorescence (in the dark).
Leaf temperature (°C): During the mid-day time, and in the absence of cloud cover, a portable steady state the promoter (LI-COR model LI- 1600) was used to measure leaf temperature on a central portion of fully extended flag leaves from three randomly selected plants in each plot.

Khedr R.A., Sorour S.G., Aboukhadrah S.H., El Shafey N.M., Abd Elsalam H.E., El-Sharnouby M.E, & El-Tahan A.M. (2021). Alleviation of salinity stress effects on agro-physiological traits of wheat by auxin, glycine betaine, and soil additives. Saudi Journal of Biological Sciences, 29(1), 534-540.

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Measuring Photosynthetic Efficiency in Plants

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The efficiency of photosystem II (Fv/Fm) was measured in leaves by a chlorophyll fluorometer (OS-30, OPTISCIENCES, USA) at flowering stage, just before irrigation of each plot. Fluorescence emission was monitored from the upper surface of the leaves. Dark-adapted leaves (30 min.) of a plant in each pot were initially exposed to the weak modulate measuring beam, and then exposes to saturated white light and initial (F 0 ) and maximum (F m ) fluorescence values were recorded. Variable fluorescence (F v ) was calculated as:

Ghassemi‐Golezani K., & Mamnabi S. (2020). SOME PHYSIOLOGICAL RESPONSES AND YIELD OF MAIZE AFFECTED BY SEED AGING AND PRIMING DURATION. Plant Breeding and Seed Science.

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Assessing Vegetable Crop Nutrient Status

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At the end of the experiment, plant height (cm) and number of branches (n) were investigated. Random samples of five C. annuum, from each plot, were taken for determining leaves chlorophyll (mg 100 g fresh weight), dry matter (%), nitrogen (N), phosphorus (P), and potassium (K) contents (mg 100 g dry weight). Total chlorophyll content (µE = µMol m⁻² s⁻¹) of fresh leaves was calculate by using chlorophyll fluorometer (model OPTI-SCIENCES OS-30), Opti-sciences, Inc. NH, USA. Impurities were removed from each plot’s samples, which were then washed in purified water and oven-dried at 70 °C until they reached a steady weight. Then, the percentages of each leaf dry matter contents (%) were calculated. Total nitrogen, total phosphorus, and total potassium contents were figured out based on the dry matter of leaves. Total nitrogen and total phosphorus contents were checked calorimetrically, using a spectrophotometer at OD₆₆₂ and OD₆₅₀ nm [46 (link)], while total potassium was determined by atomic absorption spectrometry [47 ].

Ashour M., Hassan S.M., Elshobary M.E., Ammar G.A., Gaber A., Alsanie W.F., Mansour A.T, & El-Shenody R. (2021). Impact of Commercial Seaweed Liquid Extract (TAM®) Biostimulant and Its Bioactive Molecules on Growth and Antioxidant Activities of Hot Pepper (Capsicum annuum). Plants, 10(6), 1045.

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Evaluating Vegetative Growth and Yield of Pepper Plants

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Five pepper plants per plot were randomly sampled at three months after transplanting to determine the vegetative in terms of; plant height (cm) and number of branches per plant. In addition, leaf chemical constituents were determined as total chlorophyll content (mg g⁻¹ fresh weight) by using chlorophyll fluorometer (model OPTI-SCIENCES OS-30), Opti-sciences, Inc. USA. Dry matter content (mg/100 g fresh weight) was determined by drying the leaves at 70 °C until constant weight was reached.
Hot pepper fruits at the colored marketable stage were harvested twice weekly along each growing season to determine total yield (t h⁻¹). At harvesting, a sample of five randomly chosen fruits of each experimental plot from the second harvest were taken to determine fruit’s physical characteristics in terms of fruit length (cm) and fruit diameter (cm). Samples of hot pepper fruits of the second growing season were subjected to experimental lab to determine decay rate after postharvest infection with Alternaria alternata.

Hassan S.M., El-Bebany A.F., Salem M.Z, & Komeil D.A. (2021). Productivity and Post-Harvest Fungal Resistance of Hot Pepper as Affected by Potassium Silicate, Clove Extract Foliar Spray and Nitrogen Application. Plants, 10(4), 662.

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Leaf Chlorophyll Content Measurement

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Freshly picked leaves from ten plants of each treatment were used to determine the total chlorophyll content of the leaves (mg.100 g m -1 f.w.). The total chlorophyll content (µE = µMol m -2 s -1 ) of fresh leaves was determined by using a chlorophyll fluorometer (model OPTI-SCIENCES OS-30; Opti-sciences, Inc., Hudson, NH, USA).

Hassan S.M., Ashour M., Sakai N., Zhang L., Hassanien H.A., Gaber A., & Ammar G.A.G. (2021). Impact of Seaweed Liquid Extract Biostimulant on Growth, Yield, and Chemical Composition of Cucumber (Cucumis sativus). Agriculture, 11(4), 320-320.

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