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Deltavision elite dv imaging system

Manufactured by GE Healthcare
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The Deltavision Elite DV imaging system is a high-resolution fluorescence microscopy platform designed for advanced live-cell and fixed-sample imaging. It features an inverted microscope configuration, and supports a range of imaging modalities, including widefield, deconvolution, and advanced techniques such as FRET and FLIM.

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Lab products found in correlation

Softworx software, by Cytiva (1 mentions) Immuno mount medium, by Thermo Fisher Scientific (1 mentions) Scmos 2048 2048 pixels2 camera, by GE Healthcare (1 mentions) Prolong gold antifade, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 phalloidin, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

DeltaVision Elite (137 citations) DeltaVision Elite system (48 citations) DeltaVision imaging system (24 citations) DeltaVision Elite Imaging System (20 citations) DeltaVision system (15 citations) Deltavision Elite DV (3 citations) Deltavision DV (2 citations)

2 protocols using deltavision elite dv imaging system

1

Immunofluorescence Imaging of Cellular Structures

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Cells were fixed, permeabilized and blocked using Methanol (−20 °C, 10 min), 0.5% Nonidet-P40 (Bioshop, 20 min), and 0.2% Fish Gelatin/PBS X1h. Antibody incubations were in the blocking solution overnight (4 °C), and slides were mounted in Immuno-mount medium (Thermo). Immunofluorescence images were collected using the Olympia Deconvolution fluorescence microscope or Deltavision Elite DV imaging system equipped with a sCMOS 2048×2048 pixels2 camera (GE Healthcare). Z stacks (0.2 μm apart) were used, and images were deconvolved and maximum intensity projected using softWoRx software (Applied Precision). Images were collected using 60X and 100×1.4 NA oil objectives (Olympus). High- and super-resolution imaging were performed following standard procedures69 (link) at the Network Biology Collaborative Centre (NBCC), a facility supported by Canada Foundation for Innovation, the Ontarian Government, and Genome Canada and Ontario Genomics Institute (OGI-139).
Kazazian K., Haffani Y., Ng D., Lee C.M., Johnston W., Kim M., Xu R., Pacholzyk K., Zih F.S., Tan J., Smrke A., Pollett A., Wu H.S, & Swallow C.J. (2020). FAM46C/TENT5C functions as a tumor suppressor through inhibition of Plk4 activity. Communications Biology, 3, 448.
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2

Fluorescent Staining of Centrin, PCNT, and F-Actin

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Centrin and PCNT staining were performed following standard protocols as
previously described (Liu et al., 2018 ).
For the Phalloidin staining, cells were fixed by 4% PFA at room temperature for
20 minutes and treated with permeabilization buffer (0.5 % Triton X-100 in PBS)
for another 10 minutes. Cells were then blocked in 1% BSA in PBS for one hour
and incubated with Alexa Fluor 488 Phalloidin (Invitrogen A12379) and DAPI in
blocking solution for another one hour. After a final wash three times with PBS
for 5 minutes each, cells were inverted and mounted on glass slides with
standard mounting solution (ProLong Gold antifade, Molecular Probes). Cells were
imaged on a Deltavision Elite DV imaging system (GE Healthcare) equipped with a
sCMOS 2048x2048 pixels2 camera (GE Healthcare). Z stacks (0.2 μm apart)
were collected, and images were deconvolved and projected using softWoRx (v6.0,
Applied Precision).
Liu Y., Kim J., Philip R., Sridhar V., Chandrashekhar M., Moffat J., van Breugel M, & Pelletier L. (2020). Direct interaction between CEP85 and STIL mediates PLk4-driven directed cell migration. Journal of cell science, 133(8), jcs238352.
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