Rabbit anti lamp1 antibody

Manufactured by Cell Signaling Technology

Sourced in United Kingdom

Rabbit anti-LAMP1 antibody is a primary antibody that specifically recognizes the Lysosome-Associated Membrane Protein 1 (LAMP1), a glycoprotein found in the membrane of lysosomes. This antibody can be used to detect and visualize LAMP1 in various experimental techniques, such as Western blotting, immunohistochemistry, and immunofluorescence.

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Close spelling variants of this product

LAMP1 (111 citations) Anti-LAMP1 (49 citations) Anti-LAMP1 antibody (18 citations) Rabbit anti-LAMP1 (13 citations) LAMP1 antibody (8 citations)

3 protocols using rabbit anti lamp1 antibody

Immunofluorescence Labeling of Cellular Proteins

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The primary antibodies used in this study were as follows: mouse anti-GFP antibody (Roche Life Science, Switzerland), mouse anti-HA antibody (Roche Life Science), mouse anti-PGK1 monoclonal antibody (Abcam, United Kingdom), rabbit anti-LAMP1 antibody (Cell Signaling Technology, Danvers, MA, United States), rabbit anti-Vps53 antibody (Atlas Antibodies, Sweden), and mouse anti-tubulin antibodies (Sigma, St. Louis, MO, United States). Secondary antibodies used for immunofluorescence were HRP-conjugated (horseradish peroxidase) anti-mouse and anti-rabbit IgG (Santa Cruz Biotechnology, Dallas, TX, United States) and Alexa 488–conjugated anti-rabbit IgG (Invitrogen/Thermo, Waltham, MA, United States).

Fröhlich F., Petit C., Kory N., Christiano R., Hannibal-Bach H.K., Graham M., Liu X., Ejsing C.S., Farese RV J.r, & Walther T.C. (2015). The GARP complex is required for cellular sphingolipid homeostasis. eLife, 4, e08712.

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Immunofluorescence Staining of Cellular Organelles

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Culture medium was evacuated from 384 well imaging plates with a BioTek EL406 plate washer. Cells were fixed with cold methanol at − 20 °C for 15 min. Plates were washed once with 1xPBS before primary antibody incubation at 4 °C overnight in staining solution: 1xPBS, 1% BSA (Thermo Fisher Scientific), 0.2% Triton X-100 (Sigma-Aldrich), 5% goat serum (Jackson ImmunoResearch). Secondary staining was performed in PBS with addition of HOECHST 33,342 (Thermo Fisher Scientific). Plates were washed 3 × with PBS between staining steps. Primary antibodies were: mouse anti-p62 antibody (1:1000 dilution, MBL International, #M162-3); rabbit anti-LAMP1 antibody (1:1000, Cell Signaling Technology, #9091S). Secondary antibodies: highly cross-adsorbed secondary antibodies goat anti-mouse IgG (H + L) Alexa Fluor 568/647 and goat anti-rabbit IgG (H + L) Alexa Fluor 647 (Thermo Fisher Scientific).

Duan L., Hu M., Tamm J.A., Grinberg Y.Y., Shen F., Chai Y., Xi H., Gibilisco L., Ravikumar B., Gautam V., Karran E., Townsend M, & Talanian R.V. (2021). Arrayed CRISPR reveals genetic regulators of tau aggregation, autophagy and mitochondria in Alzheimer’s disease model. Scientific Reports, 11, 2879.

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Molecular Regulation of Tissue Factor

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Phorbol 12-myristate 13-acetate (PMA, #P8139), Actinomycin D (#A9415), Cyclohexamide (#C7698), and MG132 (#M7449), were purchased from Sigma. Chloroquine (#TLRL-CHQ) was from InvivoGen. Go 6983 (#2285) and GF 109203X (#0741) were from Tocris. Rabbit anti-TF antibody (#NBP2–15139) was from Novus Biologicals. Mouse anti-TF antibody (#H00002152-B01P) was from Abnova. Mouse anti-actin antibody (#612656) was from BD Transduction Laboratories. Rabbit anti-LAMP-1 antibody (#9091) was from Cell Signaling Technologies. Anti-rabbit (#711–036-152) and anti-mouse (#115–036-146) HRP-conjugated secondary antibodies were from Jackson Immunoresearch. Anti-mouse AF488-conjugated (#A32723) and anti-rabbit AF594-conjugated (#A-11072) secondary antibodies, DAPI (#D1306), sulfo-NHS-SS-Biotin (#21331), streptavidin beads (#20347), BCA protein assay kit (#23225), and TF inhibitory antibody (#16–1429-82) were from Fisher Scientific. RIPA with protease/phosphatase inhibitors (#sc-24948A) was from Santa Cruz Biotechnology. Enhanced chemiluminescence kit (#34080), TRIzol (#15596018), cDNA synthesis kit (#4387406), and Taqman assays (#4331182) were from Life Technologies. FX (HCS-0050) and FVIIa (HCVIIA-0031) were from Haematological Technologies. Pefachrome FXa (#085–06) was from Pentapharm.

Sommerville L.J., Gorman K.L., Snyder S.A., Monroe D.M, & Hoffman M. (2019). A Unique Protein Kinase C-Dependent Pathway for Tissue Factor Downregulation in Pericytes. Journal of thrombosis and haemostasis : JTH, 17(4), 670-680.

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