Body weight was monitored every week post-surgery (week 0). On week three, animals were separated into individual cages to adapt to their surroundings for one day. Food intake was measured on the third day. During week 6, all the animals were placed into metabolic cages (Columbus Instruments, Comprehensive Lab Animal Monitoring System (CLAMS), OH, USA) to adapt to their surroundings 12 hours before measurement. All metabolic data was collected in night phase. For the insulin tolerance test during week 6, animals were fasted overnight. The baseline plasma glucose was measured by glucometer (FreeStyle Lite system, Abbott Diabetes Care Inc. CA, USA) at t=0. Then 0.75U/kg insulin was systemically injected immediately after baseline samples. The plasma glucose was measured at t=15, 30, 60, and 120 min after insulin injection.
Freestyle lite system
Manufactured by Abbott
Sourced in United States
The FreeStyle Lite system is a compact and portable blood glucose monitoring device designed for people with diabetes. It provides blood glucose measurements to help users manage their condition.
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Lab products found in correlation
3 protocols using freestyle lite system
1
Metabolic Profiling and Insulin Sensitivity
2
Insulin Tolerance Test in Fasted Mice
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ITT was conducted in mice after fasting of 6 hours as described elsewhere 45 (link). At Time 0, blood glucose was measured to set a baseline. insulin (1 unit/kg ITT) was injected i.p., blood samples were collected from the tail vein at 15, 30, and 60 minutes. The plasma glucose was measured by a glucometer (FreeStyle Lite system, Abbott Diabetes Care CA, USA).
3
Quantifying Metabolic Markers in Mice
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For blood glucose measurement, a drop of tail blood was applied by glucometer (FreeStyle Lite system, Abbott Diabetes Care Inc., CA, USA). For insulin measurement, about 150 μl of trunk blood was collected before scarification. The plasma samples were harvested and froze until ELISA by centrifuging the blood samples (with anticoagulant) at 1500 rpm for 15 min (4°C). ELISA was performed following the procedure provided by ELISA kits (EMINS, Thermo Fisher Scientific).
For GLP-1 measurement, we express AAV-DIO-hM3Dq in the NTS of Gcg-Cre mice. After intraperitoneally injecting DREADD agonist CNO, we collected the fresh DMH brain slice (300 μm slice × 2) in lysis buffer and then performed ELISA (CSB-E08118, CUSABIO) according to the provided procedure with kits.
For GLP-1 measurement, we express AAV-DIO-hM3Dq in the NTS of Gcg-Cre mice. After intraperitoneally injecting DREADD agonist CNO, we collected the fresh DMH brain slice (300 μm slice × 2) in lysis buffer and then performed ELISA (CSB-E08118, CUSABIO) according to the provided procedure with kits.
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