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Talos 120

Manufactured by Thermo Fisher Scientific
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Sourced in United States, Switzerland, Germany

The Talos 120 is a high-performance transmission electron microscope (TEM) designed for a wide range of applications in materials science, life sciences, and nanotechnology. It features a 120 kV accelerating voltage and advanced imaging capabilities to enable detailed structural and compositional analysis of samples at the nanoscale.

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Lab products found in correlation

Maps software package, by Thermo Fisher Scientific (1 mentions) Maps software, by Thermo Fisher Scientific (1 mentions) Ultracut ultramicrotome, by Reichert Technologies (1 mentions)

4 protocols using talos 120

1

Quantitative Analysis of Intercellular Spaces

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Specimens were routinely processed for TEM analysis and examined by Talos 120 electron microscope (ThermoFisher Scientific, Rockford, IL, USA).
The quantitative analysis of intercellular spaces in both the basal and the suprabasal compartments was performed on ultrathin sections by ImageJ 1.53 on at least 10 random fields per sample, and results were expressed as the mean of intercellular distance (µm) + 1 SD.
Donetti E., Riva F., Indino S., Lombardo G., Baruffaldi Preis F., Rosi E, & Prignano F. (2023). Th2 Cytokines Affect the Innate Immune Barrier without Impairing the Physical Barrier in a 3D Model of Normal Human Skin. Journal of Clinical Medicine, 12(5), 1941.
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2

Virus Characterization by Electron Microscopy

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For the analysis of purified viruses, 5 μl of glycerol-free purified virus was mounted onto carbon-coated grids for 5 min. Grids were washed three times with distilled water and stained for 30 s with 10 μl of a 2% aqueous uranyl acetate solution. Samples were imaged in a CM100 transmission electron microscope at 80 keV (Thermo Fisher Scientific, Eindhoven, The Netherlands).
High-pressure frozen samples were imaged in a Talos 120 transmission electron microscope at an acceleration voltage of 120 kV using a Ceta digital camera and the Maps software package (Thermo Fisher Scientific). Overviews were acquired at a pixel size of 117 nm; detailed imaging of the samples was performed at a pixel size of 0.8 nm with a defocus of −2 μm. Images were quantified manually using Maps Viewer version 3.14.
Bauer M., Gomez-Gonzalez A., Suomalainen M., Schilling N., Hemmi S, & Greber U.F. (2021). A viral ubiquitination switch attenuates innate immunity and triggers nuclear import of virion DNA and infection. Science Advances, 7(51), eabl7150.
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3

Sapphire Disks for High-Pressure Freezing

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Cells were grown on 6 mm sapphire disks (100 µm thickness, Engineering Office M. Wohlwend, Switzerland) coated with poly-l-lysine and gold-sputtered fiducial marks, and underwent high-pressure freezing, processing, and embedding, as previously described.30 (link) Samples were imaged using a Talos 120 transmission electron microscope at 120 kV acceleration voltage equipped with a bottom mounted Ceta camera using Maps software (ThermoFisher).
Pearson A., Haenni D., Bouitbir J., Hunt M., Payne B.A., Sachdeva A., Hung R.K., Post F.A., Connolly J., Nlandu-Khodo S., Jankovic N., Bugarski M, & Hall A.M. (2022). Integration of High-Throughput Imaging and Multiparametric Metabolic Profiling Reveals a Mitochondrial Mechanism of Tenofovir Toxicity. Function, 4(1), zqac065.
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4

Ultrastructural Analysis of Liver Mitochondria

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Liver fragment from each genotype (n = 3/group) was obtained and fixed in 3% glutaraldehyde (Acros Organics, Thermo Fisher Scientific, Waltham, MA, USA) diluted in 0.1 M Sorensen phosphate buffer (pH 7.4) at 4 °C overnight. Subsequently, the livers were post-fixed with 1% osmium tetroxide in 0.1 M Sorensen phosphate buffer for 30 min, dehydrated, and araldite embedded (Fluka, Sigma Aldrich, St. Louis, MO, USA). Ultrathin sections were obtained with an Ultra-cut ultramicrotome (Reichert-Jung, Leica, Microsystems GmbH, Wetzlar, Germany), stained with uranyl acetate and lead citrate, and observed with Talos 120 (Thermo Fisher Scientific). The TEM images were acquired at 6700X magnification in randomly selected fields. Results are expressed as diameter (µm) and number of mitochondria per area for each analyzed section and were quantified by ImageJ software [36 (link)].
Svecla M., Da Dalt L., Moregola A., Nour J., Baragetti A., Uboldi P., Donetti E., Arnaboldi L., Beretta G., Bonacina F, & Norata G.D. (2024). ASGR1 deficiency diverts lipids toward adipose tissue but results in liver damage during obesity. Cardiovascular Diabetology, 23, 42.
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