Fv1000 and ix81

Manufactured by Olympus

Sourced in Japan

The FV1000 is a versatile confocal microscope system designed for a wide range of applications. It features advanced imaging capabilities, high-resolution optics, and a modular design. The IX81 is a fully motorized inverted microscope platform that provides a stable and flexible foundation for various imaging techniques, including fluorescence and phase contrast.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Beyotime (2 mentions) Rhodamine phalloidin, by Cytoskeleton (1 mentions) Maitai ehp deepsee, by Spectra-Physics (1 mentions) A1r mp, by Nikon (1 mentions) Maitai, by Spectra-Physics (1 mentions) Mouse anti na k atpase, by Merck Group (1 mentions) Minute plasma membrane protein isolation and cell fractionation kit, by Invent Biotechnologies (1 mentions) Rabbit anti tubulin, by Abcam (1 mentions) Mouse anti anxa2 monoclonal antibody, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

FV1000 (3240 citations) FV1000-IX81 (168 citations)

4 protocols using fv1000 and ix81

Laser-Scanning Microscopy for Neuronal Imaging

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The cultured
neurons were placed on an inverted laser-scanning microscope (FV1000
and IX81; Olympus Corporation, Tokyo, Japan) equipped with a water-immersion
objective lens (UPlanApo60xW/IR; N.A. 1.2; Olympus Corporation) and
a femtosecond laser (Mai Tai; Spectra Physics Inc., Mountain View,
CA, USA). Tunable wavelengths were set at 950 and 720 nm for imaging
and uncaging, respectively.

Jakkampudi S., Abe M., Komori N., Takagi R., Furukawa K., Katan C., Sawada W., Takahashi N, & Kasai H. (2016). Design and Synthesis of a 4-Nitrobromobenzene Derivative Bearing an Ethylene Glycol Tetraacetic Acid Unit for a New Generation of Caged Calcium Compounds with Two-Photon Absorption Properties in the Near-IR Region and Their Application in Vivo. ACS Omega, 1(2), 193-201.

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Visualizing MbovP280 Binding to BoMac Cells

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BoMac cells (1 ×10⁵) were propagated overnight on a microscope coverslip in each well of a 12-well-plate. To observe the binding of MbovP280 to BoMac, 0.5 μM MbovP280 was added to each well and incubated for 1 h at 37 °C. Phosphate-buffered saline (PBS) was used as the negative control. After the medium was removed, all the cells on the coverslips were washed three times with PBS, fixed with 4% paraformaldehyde in PBS for 10 min, and permeabilized with 0.5% Triton X-100 for 5 min at room temperature. All the cells were then blocked with 1% (w/v) bovine serum albumin (BSA) in PBS for 2 h. The cells were immunolabeled with mouse antiserum directed against rMbovP280 (1:500), and an Alexa-Fluor-488-conjugated goat anti-mouse IgG (H+L) secondary antibody (1:1,000) (Southern Biotech, MI, USA). Finally, the nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) 5 mg/ml (Beyotime, Shanghai, China), and the polymerized form of actin was labeled with rhodamine phalloidin (100 nM) (Cytoskeleton, CO, USA). Finally, the slides were cover-slipped and observed with a confocal laser fluorescence microscope (Olympus FV1000 and IX81, Tokyo, Japan).

Zhao G., Zhu X., Zhang H., Chen Y., Schieck E., Hu C., Chen H, & Guo A. (2021). Novel Secreted Protein of Mycoplasma bovis MbovP280 Induces Macrophage Apoptosis Through CRYAB. Frontiers in Immunology, 12, 619362.

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Visualizing Exocytosis in Pancreatic Cells

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The isolation of pancreatic acinar cells and β cells was described previously (Nemoto et al., 2001 (link); Takahashi et al., 2002 (link)). ZG exocytosis in the control and AcKO acinar cells or insulin granule exocytosis in the control and PcKO β cells was visualized using a solution that contained 0.5 or 0.7 mM SRB as a fluid-phase tracer. Two-photon excitation imaging for pancreatic acinar cells was performed using a laser-scanning microscope (A1R MP+; Nikon) with an XLPLan 25× objective lens (NA 1.05; Olympus) and a femtosecond laser (Mai Tai eHP DeepSee; Spectra-Physics). For pancreatic β cells, two-photon imaging was performed using an inverted laser-scanning microscope (FV1000 and IX81; Olympus) equipped with a water-immersion objective lens (UPlanApo 60× W/IR; NA 1.2; Olympus) and a femtosecond laser (Mai Tai; Spectra-Physics). Exocytosis was measured in response to 100 pM CCK or 20 mM glucose within arbitrary areas of the isolated acinar cells and islets. Increases in the cytosolic Ca²⁺ concentration were measured using the Fura-2 AM (K_Ca: 0.2 µM) or Fura-2 FF (K_Ca: 40 µM) Ca²⁺ indicator and were reported as (F₀ – F)/F₀, where F₀ and F represent the resting and post-stimulation fluorescence levels, respectively.

Kunii M., Ohara-Imaizumi M., Takahashi N., Kobayashi M., Kawakami R., Kondoh Y., Shimizu T., Simizu S., Lin B., Nunomura K., Aoyagi K., Ohno M., Ohmuraya M., Sato T., Yoshimura S.I., Sato K., Harada R., Kim Y.J., Osada H., Nemoto T., Kasai H., Kitamura T., Nagamatsu S, & Harada A. (2016). Opposing roles for SNAP23 in secretion in exocrine and endocrine pancreatic cells. The Journal of Cell Biology, 215(1), 121-138.

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Subcellular Localization of ANXA2 in M. bovis-infected Cells

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To see the effects of M. bovis on location of ANXA2, the plasma membrane and cytosolic proteins of the mock- and M. bovis- infected EBL cells at 12 hpi were isolated using Minute™ plasma membrane protein isolation and cell fractionation kit (Invent Biotechnologies, Plymouth, Minnesota, USA) as manufacturer’s recommendations. The abundance of ANXA2 on the cell surface and in the cytoplasm was tested by the western blot assay as described above. The following appropriate primary antibodies were used for immunodetection: mouse anti-ANXA2 monoclonal antibody (SantaCruz Biotechnology, Dallas, USA), mouse anti-Na⁺/K⁺ ATPase (sigma-Aldrich, St louis, MO, USA) and rabbit anti-tubulin (Abcam, Cambridge, UK). After incubation overnight at 4 °C, the blots were exposed to the appropriated secondary antibody. For indirect immunoflurorescence assay, cells were immunoblotted with rabbit anti-ANXA2 Ab (1:200) and with mouse anti-M. bovis 1c11 mAb (1:1000), followed with fluorescein isothiocyanate (FITC)-labled goat anti-mouse IgG (Invitrogen) and Cy3 Red-labeled goat anti-rabbit IgG (Invitrogen) as secondary antibody. DAPI was used to stain the cell nuclei (Beyotime Technology, China). Finally, the slides were covered and examined under a confocal laser fluorescence microscope (Olympus FV1000 and IX81, Tokyo, Japan).

Zhang H., Lu D., Zhang Y., Zhao G., Raheem A., Chen Y., Chen X., Hu C., Chen H., Yang L, & Guo A. (2022). Annexin A2 regulates Mycoplasma bovis adhesion and invasion to embryo bovine lung cells affecting molecular expression essential to inflammatory response. Frontiers in Immunology, 13, 974006.

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