13C3-lactic acid, 13C2-oxalic acid, 2H3-sacrosine, 2H8-valine, 13C3-dihydroxyacetone, 2H10-isoleucine, 13C4-fumaric acid, 13C4-malic acid, 2H3-aspartic acid, 13C5-glutamic acid, 13C6-4-hydroxybenzoic acid, 2H3-lauric acid, 13C5-ribose, 13C2-taurine, 2H4-citric acid, 2H7-ornithine, 13C6-tyrosine, 13C6-dopa, 2H6-kynurenine, 2H8-cystamine, and 13C11-tryptophan were purchased from Cambridge Isotope Laboratories, Inc. (MA, USA). 2H3-2-hydorxybutyric acid and 2-isopropylmalic acid were purchased from CDN isotopes (Quebec, CA) and Sigma Aldrich (Tokyo, Japan), respectively. The compounds were dissolved in methanol, as shown in Supplementary Table 3 , and then the obtained solution was used as an extraction solution. A standard alkane series mixture (C7 to C33) and octafluoronaphthalene (OFN) were purchased from Restek Co. (PA, USA) and Shimadzu Co. (Kyoto, Japan), respectively. Methoxyamine hydrochloride and N-methyl-N-trimethylsilyl-trifluoroacetamide (MSTFA), which were used for the derivatization, were obtained from Sigma-Aldrich and GL Science (Tokyo, Japan), respectively.
13c2 oxalic acid
Manufactured by Cambridge Isotopes
Sourced in United States
13C2-oxalic acid is a stable isotope compound used as a reference standard for analytical techniques. It provides a carbon-13 labelled version of oxalic acid, which is commonly used in various scientific applications.
Automatically generated - may contain errors
Lab products found in correlation
Methoxyamine hydrochloride, by Merck Group (1 mentions)
13c3 lactic acid, by Cambridge Isotopes (1 mentions)
13c4 fumaric acid, by Cambridge Isotopes (1 mentions)
13c5 glutamic acid, by Cambridge Isotopes (1 mentions)
13c6 tyrosine, by Cambridge Isotopes (1 mentions)
13c11 tryptophan, by Cambridge Isotopes (1 mentions)
N methyl n trimethylsilyl trifluoroacetamide, by Merck Group (1 mentions)
Coomassie plus protein assay reagent, by Thermo Fisher Scientific (1 mentions)
Nickel nitrilotriacetic acid ni nta agarose, by Qiagen (1 mentions)
2 protocols using 13c2 oxalic acid
1
Isotopically Labeled Metabolite Standards for Metabolomics Analysis
2
Isotope Analysis of Oxalate Compounds
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All chemicals and reagents
were purchased
from Fisher Scientific (Pittsburgh, PA) or Sigma-Aldrich (St. Louis,
MO), unless otherwise stated. [13C2]Oxalic acid
(99% 13C) was purchased from Cambridge Isotope Laboratories
(Tewksbury, MA), and an oxalate assay kit was obtained from Trinity
Biotech USA (Jamestown, NY). Nickel-nitrilotriacetic acid agarose
(Ni-NTA) was supplied by Qiagen (Germantown, MD). DNA primers were
synthesized by Integrated DNA Technologies, Inc. (Coralville, IA),
and DNA sequencing was performed in the DNA Sequence Core at the University
of Michigan (Ann Arbor, MI). Protein concentrations were determined
using the CoomassiePlus Protein Assay reagent obtained from ThermoFisher
Scientific (Waltham, MA), and BT Chelex 100 resin was purchased from
Bio-Rad (Hercules, CA). ICP-MS measurements of metal content were
taken at the Center for Applied Isotope Studies at the University
of Georgia (Athens, GA).
were purchased
from Fisher Scientific (Pittsburgh, PA) or Sigma-Aldrich (St. Louis,
MO), unless otherwise stated. [13C2]Oxalic acid
(99% 13C) was purchased from Cambridge Isotope Laboratories
(Tewksbury, MA), and an oxalate assay kit was obtained from Trinity
Biotech USA (Jamestown, NY). Nickel-nitrilotriacetic acid agarose
(Ni-NTA) was supplied by Qiagen (Germantown, MD). DNA primers were
synthesized by Integrated DNA Technologies, Inc. (Coralville, IA),
and DNA sequencing was performed in the DNA Sequence Core at the University
of Michigan (Ann Arbor, MI). Protein concentrations were determined
using the CoomassiePlus Protein Assay reagent obtained from ThermoFisher
Scientific (Waltham, MA), and BT Chelex 100 resin was purchased from
Bio-Rad (Hercules, CA). ICP-MS measurements of metal content were
taken at the Center for Applied Isotope Studies at the University
of Georgia (Athens, GA).
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