The hormone sensitive PC cell lines, LNCaP and 22RV1 were used for the in vitro studies. LNCaP and 22RV1 cells were maintained in RPMI 1640 and supplemented with 10% FBS and antibiotics. For the cell proliferation studies, all PC cells were cultured in Phenol-Red free RPMI 1640/10% charcoal-stripped FBS. 293T cells were obtained from the American Type Culture Collection (ATCC) and maintained in Dulbecco's Modified Eagle's Medium (DMEM) with 10% FBS and antibiotics. All cell lines were regularly screened for mycoplasma (Sigma Venor GeM Mycoplasma Detection Kit). DHEAS was obtained from BioVender R&D Products. Atorvastatin was obtained from Santa Cruz Biotechnology, and fluvastatin, pravastatin and simvastatin were purchased from Selleckchem.
Pravastatin
Manufactured by Selleck Chemicals
Pravastatin is a laboratory reagent used in scientific research and drug development. It is a synthetic statin compound that inhibits the enzyme HMG-CoA reductase, a key enzyme involved in the biosynthesis of cholesterol. Pravastatin is commonly used in research applications to study cholesterol metabolism and the effects of cholesterol-lowering drugs.
Automatically generated - may contain errors
Lab products found in correlation
Fluvastatin, by Selleck Chemicals (2 mentions)
Simvastatin, by Selleck Chemicals (2 mentions)
Atorvastatin, by Santa Cruz Biotechnology (2 mentions)
293t cell, by American Type Culture Collection (1 mentions)
Anti flotillin 1, by Santa Cruz Biotechnology (1 mentions)
Anti tmprss2, by Santa Cruz Biotechnology (1 mentions)
Lipopolysaccharide lps, by Merck Group (1 mentions)
Goat anti mouse secondary antibody, by Abcam (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
Goat anti rabbit secondary antibody, by Abcam (1 mentions)
Anti nf kb, by Cell Signaling Technology (1 mentions)
Atorvastatin, by Selleck Chemicals (1 mentions)
Anti ace2 antibody, by Abcam (1 mentions)
Abiraterone acetate, by Selleck Chemicals (1 mentions)
Zoledronic acid, by Selleck Chemicals (1 mentions)
Molm 14, by Leibniz Institute DSMZ (1 mentions)
Rosuvastatin, by Tokyo Chemical Industry (1 mentions)
Terbinafine, by Selleck Chemicals (1 mentions)
Oci aml3, by Leibniz Institute DSMZ (1 mentions)
4 protocols using pravastatin
1
Hormone-sensitive Prostate Cancer Cell Lines
2
Modulation of SARS-CoV-2 Entry Factors
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Atorvastatin (ATS) and Pravastatin (PVS), lipophilic and hydrophilic, respectively, were purchased from Selleckchem and dissolved in sterile DMSO. LPS (Lipopolysaccharides) was purchased from Sigma–Aldrich (St. Louis, MO, USA).
To obtain an adequate inflammatory stimulus, cells were treated for 48 h with ATS or PVS and subsequently stimulated with LPS 1 µg/mL for 4 h before the end of treatment in A549-hACE2 and MRC5 cells, or for 24 h in CaCO2 cells.
Anti-GAPDH, anti-phospho NfKB (Ser-536), and anti-NfKB antibodies were purchased from Cell Signalling Technology (Beverly, MA, USA). Anti-TMPRSS2 and anti-Flotillin-1 were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-ACE2 antibody and the goat anti-rabbit secondary antibody and goat anti-mouse secondary antibody were purchased from Abcam (Cambridge, UK).
To obtain an adequate inflammatory stimulus, cells were treated for 48 h with ATS or PVS and subsequently stimulated with LPS 1 µg/mL for 4 h before the end of treatment in A549-hACE2 and MRC5 cells, or for 24 h in CaCO2 cells.
Anti-GAPDH, anti-phospho NfKB (Ser-536), and anti-NfKB antibodies were purchased from Cell Signalling Technology (Beverly, MA, USA). Anti-TMPRSS2 and anti-Flotillin-1 were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-ACE2 antibody and the goat anti-rabbit secondary antibody and goat anti-mouse secondary antibody were purchased from Abcam (Cambridge, UK).
3
Prostate Cancer Cell Lines Comparison
4
AML Cell Lines and Drug Sensitivity
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Human AML cell lines, Molm14, and the relatively cytarabine‐resistant cell lines OCI‐AML3 and THP‐127 (Figure S2 ) were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen. All cells were cultured in RPMI 1640 medium with 10% FBS and 100 U/mL streptomycin/penicillin in a humidified atmosphere at 37°C and 5% CO2. Quizartinib (AC220) and cytarabine were purchased from Selleck Chemicals, dissolved in dimethyl sulphoxide (DMSO) at stock concentrations of 10 mmol/L and stored at −80°C. Rosuvastatin was purchased from Tokyo Chemical Industry. Pravastatin, terbinafine, bempedoic acid and zoledronic acid were purchased from Selleck Chemicals. Unless otherwise indicated, all drugs were dissolved in DMSO at stock concentrations of 100 mmol/L and stored at −20°C. Fluorescein isothiocyanate‐Annexin V antibody and propidium iodide were purchased from BD Pharmingen and Sigma‐Aldrich.
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