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1 881 protocols using spss version 27

1

Carcass Composition and Fatty Acid Analysis

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Data were analysed in a randomised complete block design. The variables of the carcass’ characteristics, lutein concentrations and meat fatty acids were first tested for normality and homoscedasticity with the Shapiro–Wilk and Levene’s tests, respectively. Statistical analysis was performed using the general linear model (GLM) ANOVA procedure in SPSS version 27, and the model included the treatment effect. Differences were considered significant at p < 0.05 and a tendency for significance at 0.05 < p < 0.10. The post hoc analyses were conducted with the Bonferroni comparison procedure in SPSS version 27. Factor component scores (z-scores) were calculated for the three-rib cut fat content to calculate the carcass fat content (CFC), after which the z-scores were transformed to standard scores (t-scores). Principal component factor analysis (PCA) was used to compute a succinct factor index for the carcass fat content (CFC t-score) and to describe the main effect of the feed additive treatments on the parameters measured. The measurements of the PCA plots were interpreted according to the correlations between each parameter. On the PCA plot, measurements close together are positively correlated; measurements separated by 180° are negatively correlated and measurements separated by 90° are independent [29 (link)].
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2

Oral Health and Personality Predictors

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Statistical analysis was performed with SPSS version 27 (SPSS Inc., Chicago, IL, USA). Data analysis included descriptive statistics of sociodemographic variables (mean ± standard deviation) and a Kolmogorov–Smirnov test to assess the assumption of normality, which was confirmed. Descriptive analyses and Pearson correlations were performed on the variables of oral health, eating habits, type A personality and lifestyles, and hierarchical linear regression models were used to search for predictors of DMFT. Subsequently, to investigate whether personality type A had moderating effects on the relationship between DMFT and EE, a simple moderation analysis (model 1) was performed using the Hayes PROCESS module (version 3.3) for SPSS version 27, (SPSS Inc., Chicago, IL, USA). Cronbach’s alpha was also calculated to assess the internal consistency of the instruments.
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3

Descriptive Statistics of PROVIT Gene Expression

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Descriptive Statistics of the PROVIT Gene Expression Sample According to per-protocol analysis, only participants providing a full set of serum samples (t 0 and t 2 ) were included in the PROVIT-CLOCK sample. First, the normal distribution of the variables was measured with the Kolmogorov-Smirnov test, Shapiro-Wilk test, and scale level. Depending on the normal distribution, baseline differences between the groups were analyzed with a two-tailed Students' t test, Mann-Whitney U test, or χ 2 test. Descriptive data were assessed by using means, mean rank, standard deviations and percentages of the respective variables. The description of the sample concerning clinical and sociodemographic data is shown in Table 1. Table 2 shows the medication. The level of significance was set at p < 0.05. All analyses were carried out with SPSS version 27 (SPSS Inc., Chicago, IL, USA). Visualization was performed with SPSS version 27, Me-taboAnalyst 5.0, and Figure 1 with the online tool Visme.
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4

Examining Siblings of Children with Health Conditions

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We conducted CFA using JASP (JASP Team, 2020). We used the Diagonally Weighted Least Squared (DWLS) estimator (Li, 2016) (link). We used the following indicators of good fit: non-significant chi-square test of model fit, Comparative Fit Index (CFI) and Tucker-Lewis Index (TLI) ≥ 0.95, Root Mean Square Error Approximation (RMSEA) < 0.06, and Standardized Root Mean Square Residual (SRMS) < 0.10. Missing data were imputed using the fully conditional specification method (MCMC) in SPSS version 27.
We used SPSS version 27 to conduct independent samples t-tests (two-tailed) to analyze group differences between siblings of children with pediatric health conditions and controls and calculated Hedges g as the effect size measure. Hedges g of 0.20, 0.50, 0.80, were interpreted as respectively a small, medium, and large effect size (Cohen, 1988) . We reconducted analyses using analysis of covariance (ANCOVA) to control for fathers' educational level. We used Pearson's r to investigate the degree of overlap between the PCCS-CR and the ECR-RS.
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5

Gait Analysis in Total Knee Arthroplasty

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TKA and healthy subjects were matched to the BCA-M cohort in IBM® SPSS® Version 27 for age (p = 0.3), sex (p = 1), body mass index (p = 0.9) and height (p = 0.5) from the prospectively collected database (Table 1). Gait output data were averaged using a custom MathWorks® MatLab® script and analysed in IBM® SPSS® Version 27. The Shapiro–Wilk test demonstrated that a number of variables were not normally distributed; therefore, all variables were compared using Kruskal–Wallis, then Mann–Whitney test with Bonferroni correction where differences were detected. Significance was set at α = 0.05.
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6

Optimizing Protein Recovery and Purity

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The technological processing of the samples was performed in duplicate as well as all the chemical determinations (n = 4). All the data were analysed using SPSS version 27.0. Multivariate general linear models were used to determine the influence of HPP pressure, HPP time, and extraction conditions (conventional extraction and UAE) on protein recovery, protein purity, and amino acid composition of the protein isolates. The differences between groups were further analysed by either Student’s t-test or Games–Howell post hoc tests for comparisons between 2 or more than 2 groups, respectively. In all cases, the criterion for statistical significance is p < 0.05. The variance in the data set was analysed by principal component analysis (PCA) using direct Varimax rotation with Kaiser normalisation to calculate the expected weight for each component with eigenvalues higher than 1. PCA scatter plots were generated by SPSS version 27.0, while all the other graphics were produced in Microsoft Excel.
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7

Assessing Multidimensional Harms of Substance Use

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The age, gender, and regional distributions were used to calibrate the data weights. Percentages and significance (p) were calculated with Chi-Squared and Fisher’s exact tests. All selected variables were dichotomized and added simultaneously into the logistic regression models. Furthermore, health-related harms, work/study harms, and social deviance harms were combined for the model and named other harms due to the small number of respondents in some subcategories. To optimize the model, gender and age were added. All analyses were done using SPSS version 27.0 (IBM Corporation, Chicago, IL, USA).
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8

Evaluating Sunscreen Use Behavior

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Participants’ responses were first preprocessed and then exported to IBM SPSS version 27.0 (IBM Corp. Armonk, NY, USA) for statistical analyses. Incomplete responses and those with invalid data entries were excluded. Mean and standard deviation were used to represent continuous variables. Counts and proportions were used to express categorical variables. Inferential statistics were conducted through independent samples-t-tests to perform group-wise comparisons. Cronbach’s alpha values were computed for the entire scale and subscales to assess the internal consistency. Two hierarchical regression models (HRM) were fit to explain the variance in the likelihood of initiation and sustenance of sunscreen use behavior by MTM individual constructs, besides the demographic variables. Structural equation modeling (SEM) was utilized for the construct validation. The Analysis of Moment Structure, AMOS (Chicago, IL, USA) was used for SEM [27 ]. We used indices such as chi-square (χ2), root mean square error of approximation (RMSEA), comparative fit index (CFI), and Tucker–Lewis (TLI) to assess how well our models fit the data [31 ,32 ,33 (link)]. Models were considered to have adequate fit if they met the less stringent, but traditionally accepted, values of 0.90 or greater for CFI and TLI, and values less than 0.08 for RMSEA. P-values less than 0.05 were considered statistically significant.
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9

Factors Influencing Copper IUD Attitudes

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Alongside descriptive statistics, we used multiple logistic regressions to measure any associations between negative attitudes toward or reported negative experiences of the copper IUD and sociodemographic and other variables. Associations were expressed as odds ratios (ORs) with 95% confidence intervals (95% CIs). All statistical analyses were conducted using IBM SPSS Version 27.0 (SPSS Inc., Chicago, IL, USA). Open-survey responses were analyzed thematically (75 (link)), i.e., divided into themes and categories, using NVivo.
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10

Statistical Analysis of Biological Replicates

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The data were derived from two independent experiments with biological and technical replicates and are presented as a mean + standard error of the mean (SEM). A one-way ANOVA in conjunction with the Bonferroni post-hoc test from SPSS version 27.0 (IBM Corp., Armonk, NY, USA) was used in all cases, except for the proliferation and scratch assays, where the multiple measures were also accounted for. In cases where the parametric testing of multiple samples was not permissible, the Kruskal–Wallis or Friedman non-parametric approaches were used. The significance was set at p < 0.05.
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