Mice were pre-treated with 20 mg/100 βl/mouse of streptomycin (Strep) 1 day prior to E. coli inoculation. Strep-treated mice were then inoculated with human E. coli strain HS or LF82 (1 × 109 CFU). Seven days after E. coli colonization, mice were infected with 2×107 CFU of S. Typhimurium SL1344 ΔspiA. To determine bacterial numbers in the feces, fecal pellets were collected from individual mice, homogenized in cold PBS, and plated at serial dilutions on MacConkey agar plates containing 50 µg/ml Strep or ampicillin (Amp). CFU counts were determined after overnight incubation at 37°C. IL-33-ST2 signaling in vivo was blocked by the intraperitoneal administration of 50 µg of an anti-mouse ST2 antibody or control rat IgG (Genentech. Inc) 3 times per week. 21 days following Salmonella infection, colonic tissues were harvested and fixed in buffered 4% paraformaldehyde. Fixed tissues were then embedded in paraffin, sectioned, and stained with heamatoxylin and eosin or picro-sirius red (Picrosciences, Inc.).
Anti mouse st2 antibody or control rat igg
Manufactured by Genentech
The Anti-mouse ST2 antibody or control rat IgG is a laboratory equipment product. The Anti-mouse ST2 antibody is a primary antibody that specifically binds to the mouse ST2 protein. The control rat IgG is an isotype control antibody derived from rat. These products are intended for use in research applications.
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Lab products found in correlation
2 protocols using anti mouse st2 antibody or control rat igg
1
Modulation of Gut Microbiome and Immune Responses
2
Modulation of Gut Microbiome and Immune Responses
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Mice were pre-treated with 20 mg/100 βl/mouse of streptomycin (Strep) 1 day prior to E. coli inoculation. Strep-treated mice were then inoculated with human E. coli strain HS or LF82 (1 × 109 CFU). Seven days after E. coli colonization, mice were infected with 2×107 CFU of S. Typhimurium SL1344 ΔspiA. To determine bacterial numbers in the feces, fecal pellets were collected from individual mice, homogenized in cold PBS, and plated at serial dilutions on MacConkey agar plates containing 50 µg/ml Strep or ampicillin (Amp). CFU counts were determined after overnight incubation at 37°C. IL-33-ST2 signaling in vivo was blocked by the intraperitoneal administration of 50 µg of an anti-mouse ST2 antibody or control rat IgG (Genentech. Inc) 3 times per week. 21 days following Salmonella infection, colonic tissues were harvested and fixed in buffered 4% paraformaldehyde. Fixed tissues were then embedded in paraffin, sectioned, and stained with heamatoxylin and eosin or picro-sirius red (Picrosciences, Inc.).
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