The prednisolone was purchased from Sigma-Aldrich (St. Louis, MO, USA) and the absolute ethanol was purchased from Scharlau (Barcelona, Spain). The saturated prednisolone solution was prepared by putting the excess amount of prednisolone into absolute ethanol. The addition of prednisolone was stopped only if oversaturation was observed after shaking of 10 mins (Fisher Vortex Genie 2, Hampton, NH, USA). The oversaturated solution was then centrifuged at 14,000 rpm for 5 min (Sigma 3-18K, Tokyo, Japan) and the saturated prednisolone was removed and ready for use. The prednisolone solution was later used to mix with deionized (DI) water in the microchannel.
Prednisolone
Manufactured by Merck Group
Sourced in United States, Japan, United Kingdom, Italy, Germany, Sao Tome and Principe, Denmark
Prednisolone is a synthetic corticosteroid used in the Merck Group's laboratory equipment. It functions as an anti-inflammatory and immunosuppressant agent.
Automatically generated - may contain errors
Lab products found in correlation
Dexamethasone (dex), by Merck Group (21 mentions)
Cortisol, by Merck Group (10 mentions)
Prednisone, by Merck Group (8 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (7 mentions)
Penicillin streptomycin, by Merck Group (6 mentions)
Testosterone, by Merck Group (6 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (6 mentions)
Cortisone, by Merck Group (5 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (4 mentions)
Doxorubicin, by Merck Group (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Streptomycin, by Thermo Fisher Scientific (4 mentions)
Betamethasone, by Merck Group (3 mentions)
Non essential amino acids (neaa), by Merck Group (3 mentions)
Mg132, by Merck Group (3 mentions)
Hek293 cell, by American Type Culture Collection (3 mentions)
Hepg2 cell, by American Type Culture Collection (3 mentions)
L glutamine, by Merck Group (3 mentions)
Corticosterone, by Merck Group (3 mentions)
Acetonitrile, by Merck Group (3 mentions)
103 protocols using prednisolone
1
Prednisolone Saturation Protocol
2
Chronic Hypoxia and Pharmacological Treatments
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Chronic hypoxic rearing was performed as previously described [35 (link), 48 (link), 53 (link), 54 (link)]. Briefly, litters of the C57Bl6/J strain were culled to a size of, at most, ten pups and co-fostered with CD1 or Swiss Webster strain dams then reared at 10% O2 in a hypoxic chamber (Biospherix, Inc., Laconia, NY) from postnatal day 3 (P3) to P11. Tissue from P4, P7, P11, P22, or P40 was then harvested acutely for analysis. On P3, pups received daily intraperitoneal injections of prednisolone (0.67 g/kg body weight, Sigma-Aldrich), SAG (20 g/kg body weight), prednisolone in combination with SAG, or vehicle (DMSO), for 8 days, or the duration of the hypoxic experiment. For acute treatment, a one-time dose of SAG was given at P11.
3
Synthesis and Characterization of HA and PNAGA Polymers
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30 KDa HA,Na (HA30) and 100 KDa HA,Na (HA100) were kindly supplied by Novozymes (Copenhagen, Denmark), whereas 1,5-2,2 MDa HA,Na (HA2000) was purchased at Acros Organics Fisher Scientific (Ilkirch, France). NAGA monomer was synthesized from acryloyl chloride and glycinamide in water according to a protocol reported previously [14 ]. Potassium persulfate (K2S2O8), Sodium azide (NaN3), FITC-labelled poly(l -lysine) (5000–30,000 g/mol), Type I-S Hyaluronidase from bovine testes as lyophilized powder (400–1000 units/mg), Prednisolone, and PBS Dulbecco (PBS) were all purchased at Sigma-Aldrich (Saint-Quentin Fallavier, France). The model PNAGA121 polymer with MW = 121,000 g/mol was synthesized in the laboratory.
4
Synthesis and Extraction of Cysteinyl Leukotriene-1 Receptor Antagonist
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JTE-852 was synthesized and cysteinyl leukotriene-1 receptor antagonist pranlukast was extracted from ONON® capsules (Ono Pharmaceutical Co., Ltd., Osaka, Japan) in the Central Pharmaceutical Research
Institute of Japan Tobacco, Inc. (Osaka, Japan). The chemical structure of JTE-852 has been previously reported [11 (link)]. Histamine H1-receptor antagonist ketotifen and steroidal drug
prednisolone were purchased from Sigma-Aldrich, Co. (St. Louis, MO, U.S.A.). Metolose® SM-1500 (methylcellulose) was purchased from Shin-Etsu Chemical Co., Ltd. (Tokyo, Japan) and its 0.5% aqueous solution was
used as a vehicle.
Institute of Japan Tobacco, Inc. (Osaka, Japan). The chemical structure of JTE-852 has been previously reported [11 (link)]. Histamine H1-receptor antagonist ketotifen and steroidal drug
prednisolone were purchased from Sigma-Aldrich, Co. (St. Louis, MO, U.S.A.). Metolose® SM-1500 (methylcellulose) was purchased from Shin-Etsu Chemical Co., Ltd. (Tokyo, Japan) and its 0.5% aqueous solution was
used as a vehicle.
5
Prednisolone Administration in DMEM
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Dulbecco’s modified Eagle’s medium (DMEM), Dulbecco’s phosphate-buffered saline (DPBS), and fetal bovine serum (FBS) were purchased from Life Technologies. Prednisolone (Sigma) was given orally for 5 days at 1 mg/kg.
6
CIM Therapeutic Treatment with PF1801 and Prednisolone
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For the treatment of CIM with PF1801 (ImmunoForge, Seoul, Korea) and/or prednisolone (Sigma, St. Louis, Montana, USA), PF1801 was dissolved in phosphate buffered salts (PBS) and PF1801 or the control vehicle (PBS) was subcutaneously administered every day from day 0 of CIM to day 14 or from day 7 of CIM, just after the measurement of grip strength, to day 21 for the prophylactic treatment or therapeutic treatment, respectively. prednisolone (PSL; Sigma‐Aldrich, St. Louis, Montana, USA) was dissolved in 0.5% (w/v) methylcellulose (Wako, Tokyo, Japan) and 5% (w/v) gamma cyclodextrin (Tokyo Chemical Industry, Tokyo, Japan). 20 mg/kg body weight (BW) of PSL or the control vehicle (0.5% methylcellulose and 5% gamma cyclodextrin) was orogastrically administered every day from day 0 of CIM to day 14 or from day 7 of CIM, just after the measurement of grip strength, to day 21 for the prophylactic treatment or therapeutic treatment, respectively. The mice were randomly allocated to each treatment or control group after the induction of CIM. Each cage housed the mice taking different treatments to minimize potential confounders.
7
Endothelial Cell Response to Prednisolone
8
Mdx Mice Treatment with Spironolactone and Prednisolone
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Mdx mice were treated using water bottles containing 250 mg/L spironolactone (Sigma-Aldrich, S3378) or 6.7 mg/L prednisolone (Sigma-Aldrich, P6004), the active metabolite of prednisone dissolved in MediDrop containing sucralose (ClearH2O, 75-01-1001). Mice were treated during the peak of inflammation for 10 days for cytokine- and chemokine-level analysis to allow detection of differences in protein expression (4–5.5 weeks of age), for 7 days (3.5–4.5 weeks of age) for flow cytometry, RNA-Seq, and diaphragm IHC or for 14 days (3.5–5.5 weeks of age) for quadriceps IHC and histology. Approximate dosages for spironolactone and prednisolone treatment were 37.5 and 1 mg/kg × day, respectively (8 (link), 33 (link)). The mdx mice given MediDrop vehicle were used as controls for all experiments. Mice were housed 5 per cage and euthanized via cervical dislocation.
9
Comprehensive Inhibitor Screening Assay
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The following inhibitors and compound library were used at the indicated concentrations and durations: Prednisolone (Sigma-Aldrich, P6004), HG-9-91-01 (MedChemExpress, HY-15776), YKL-05-099 (MedChemExpress, HY-101147), YKL-06-61 (MedChemExpress, HY-120056), venetoclax (MedChemExpress, HY-15531), dasatinib (Sigma-Aldrich, CDS023389), imatinib (Sigma-Aldrich, CDS022105), ruxolitinib (SelleckChem, S1378), DAPT (SelleckChem, S2215), and the Epigenetics Compound Library (SelleckChem).
10
Steroid Solutions for Biomedical Research
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Albumin from human serum (HSA) lyophilized powder, ≥ 97% (agarose gel electrophoresis), was purchased from Sigma Aldrich (Italy). To prepare the stock solution (100 μM), HSA was dissolved in 2 mM phosphate buffer solution (PBS, pH 7.4).
Betamethasone (≥98%), flunisolide (≥97%), prednisolone (≥99%) and triamcinolone were all purchased from Sigma Aldrich (Italy); the stock solutions (3 mM) were prepared by dissolving drugs in a solution of 96% ethanol and PBS (1:1, v/v).
Betamethasone (≥98%), flunisolide (≥97%), prednisolone (≥99%) and triamcinolone were all purchased from Sigma Aldrich (Italy); the stock solutions (3 mM) were prepared by dissolving drugs in a solution of 96% ethanol and PBS (1:1, v/v).
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