Diabzi compound 3

THP1-Dual cells (Cat. code: thpd-nfis), THP1-Dual KO-STING cells (Cat. code: thpd-kostg), 293T-Dual hSTING-H232 cells (Cat. code: 293d-h232), 293T-Dual hSTING-R232 cells (Cat. code: 293d-r232), Raw-Lucia (Cat. code: rawl-isg) and Raw-Lucia-KO-STING (Cat. code: rawl-kostg) were purchased from InvivoGen (San Diego, USA). All the cells were incubated following manufacturer’s protocols at 37 ^oC in a humidified atmosphere of 5% CO₂. QUANTI-Luc solution (Cat. code: rep-qlc2) and QUANTI-Blue solution (Cat. code: rep-qbs2) were purchased from InvivoGen (San Diego, USA). Antibodies against phospho-TBK1 (Ser172) (Cat. code: 5483S), TBK1 (Cat. code: 3504S), phospho-IRF3 (Ser396) (Cat. code: 4947S), IRF3 (Cat. code: 4302S), and GAPDH (Cat. code: 5174S) were purchased from Cell Signaling Technology (Beverly, MA). diABZI-compound 3 (Cat. code: S8796) and MSA-2 (Cat. code: S9681) were purchased from Selleck (Houston, USA).

MDA-MB-231, MCF7, ZR-75-1, and HFF cell lines were purchased from the ATCC. ZR-75-1 cells were cultured in RPMI 1640 (Gibco), and the other cells were cultured in Dulbecco's modified Eagle's medium (DMEM) (Gibco) supplemented with 10% fetal bovine serum (Excell Bio) in an incubator at 37 °C with 5% CO₂. The antibody against GAPDH (sc-47724) was purchased from Senta Cruz Biotechnology. Antibodies against STING (13647), TBK1 (3504s), pTBK1 S172 (5483S), elF2α (5324S), and p-elF2α S51 (3398S) were purchased from Cell Signaling Technology. Antibodies against PLAU (ab24121) and pIRF3 S386 (ab76493) were purchased from Abcam (MA 02453, USA). Antibodies against PERK (A18196) and p-PERK T982 (AP0086) were purchased from ABclonal Technology. STING agonist diABZI (Compound 3) and PERK inhibitors GSK2656157 and GSK2606414 were purchased from Selleck Chemicals.