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Pulverisette 19 universal cutting mill

Manufactured by Fritsch
Sourced in Germany

The Pulverisette 19 Universal Cutting Mill is a laboratory equipment designed for the grinding and size reduction of various solid materials. It features adjustable rotor speed and a range of interchangeable cutting tools for efficient sample preparation.

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5 protocols using pulverisette 19 universal cutting mill

1

Hemp Hurd Preparation and Characterization

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The hemp hurd (200 g by weight) was dried at 60 °C for 12 h, followed by chopping into a coarse particle with a Fritsch Pulverisette 19 Universal Cutting Mill (sieve diameter of 200 µm). Polyvinyl alcohol solution (PVA) (5 wt. %) was purchased from Flew solution (Queensland, Australia). Sulphuric acid (98 wt. %) was purchased from Chemsupply (Adelaide, Australia).
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2

Cellulose Extraction from Hemp Hurds

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The raw material used in this study was the cellulosic waste (hemp hurds) obtained from the inner core of hemp (Cannabis sativa) biomass. The hemp hurds used in the study were grown in New South Wales, Australia (S34°34′, E146°12′) and was harvested in March 2009. The material was dried at 70 °C to obtain a constant weight and then milled in a Fritsch Pulverisette 19 Universal Cutting Mill using sieve diameter of 1 mm. The milled hemp was sieved again manually using a mesh size of 300 µm to remove bigger particles and the particle size used for the experiment varied from 300–600 µm. The chemicals used in the extraction process were sodium hydroxide (Sigma-Aldrich, St. Louis, MO, USA), sulphuric acid (98%, AR grade-Merck, Kenilworth, NJ, USA) and hydrogen peroxide (Chem-supply, Gillman, South Australia, Australia). The enzyme hydrolysis of isolated cellulose was achieved using cellulase from Trichoderma reesei (Sigma-Aldrich) [15 (link)].
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3

Hemp Hurd Biomass Milling and Sieving

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The biomass used for the study was hemp hurd (Cannabis sativa), procured as an industrial residue. The biomass was milled using a Fritsch Pulverisette 19 Universal Cutting Mill. The milled biomass was sieved using a mesh of pore size about 300 μm.
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4

Pyrolysis of Personal Protective Equipment

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The pyrolysis experiments were performed with 0.25 mm PP-IG samples. The PP-IG samples were prepared from used PPEs, including isolation gowns. Next, the collected materials were shredded using FRITSCH Universal Cutting Mill PULVERISETTE 19, Idar-Oberstein, Germany, with 0.25 mm perforation sieve cassette into 0.25 mm-size PP-IG samples. The samples then were directly used for sample characterisations and experiments of laboratory-scale pyrolysis with specified parameters.
For a comprehensive overview, the PPEs, include the isolation gowns, hairnets, shoe covers, and face masks, were collected from Universiti Putra Malaysia (UPM) Healthcare Centre (PKU), UPM Serdang, Selangor, Malaysia. Priors to shredding steps, the collected waste PPEs were disinfected thoroughly under the supervision of HCWs from UPM PKU staff. Materials collected were cleaned and disinfected using normal soap meticulously. The materials were dried under direct sunlight and shredded using the abovementioned cutting mill into small powder of size ~0.25 mm for better pyrolysis experiment. Twenty g of segregated PP-IG samples were filled into a quartz boat and placed at the centre of the furnace. The elemental composition of PP plastic is as shown in Table 1.
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5

Tamarindus indica Stem Extraction

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Fresh stems (10 kg) of T. indica were collected from the local garden Taman Pertanian, Indera Mahkota, 25,200 Kuantan, Pahang DM, Malaysia. Identification of the plant was performed by the taxonomists of Taman Pertanian and Kulliyyah of Pharmacy, IIUM. Afterward, the sample was deposited in the herbarium of Kulliyyah of Pharmacy to obtain voucher specimen number (NMPC-QSTI-39) for the future references. The same plant material was compared with the already deposited specimen of the same plant.
Ten kg well cleaned fresh stems were dried in a laboratory dryer within a temperature range (30 to 40 °C) and were pulverized to a crude powdered form by applying Fritsch Universal Cutting Mill PULVERISETTE 19 (Germany) and the grinded material was made ready for the extraction process 4.7 kg (47%) to prepare ethanol extract as well as to isolate bioactive compounds [12 , 13 (link)].
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