C mpl mice

Manufactured by Genentech

C-mpl-/- mice are genetically engineered mice that lack the c-mpl gene, which is essential for the production of megakaryocytes and platelets. These mice are commonly used in research to study the role of the c-mpl gene and its encoded protein, the thrombopoietin receptor, in the regulation of megakaryopoiesis and platelet production.

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3 protocols using c mpl mice

Genetic Mouse Models for Platelet Research

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Mice were housed and bred under specific pathogen-free conditions at the University of California, San Francisco (UCSF) Laboratory Animal Research Center and all experiments conformed to ethical principles and guidelines approved by the UCSF Institutional Animal Care and Use Committee. Male and female mice between 8–12 weeks of age were used for experiments. C57BL/6, PF4-Cre, Rosa26-LSL-tdTomato, mTmG, nTnG, and BoyJ mice were purchased from the Jackson Laboratories. To track platelets and/or MKs, PF4-Cre expressing mice were crossed with Rosa26-LSL-tdTomato, mTmG or nTnG reporter strains. The fluorophore of PF4 expressing cells (tdTomato or GFP) is localized to the cytoplasm, the cell membrane, or the nucleus, respectively. c-mpl^-/- mice (C57BL/6 background) were obtained from a Material Transfer Agreement from Genentech.

Lefrançais E., Ortiz-Muñoz G., Caudrillier A., Mallavia B., Liu F., Sayah D.M., Thornton E.E., Headley M.B., David T., Coughlin S.R., Krummel M.F., Leavitt A.D., Passegué E, & Looney M.R. (2017). The lung is a site of platelet biogenesis and a reservoir for hematopoietic progenitors. Nature, 544(7648), 105-109.

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Mouse Breeding and Genotyping Protocol

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Mice were housed and bred under specific pathogen–free conditions at the UCSF Laboratory Animal Research Center. Male and female mice between 8 and 12 weeks of age were used for the experiments. WT C57BL/6J, C57BL/6-Tg(Pf4-icre)Q3Rsko/J, B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTdTomato)Hze}/J, B6.129(Cg)-Gt(ROSA)26Sor^{tm4(ACTB-tdTdTomato,-EGFP)Luo}/J, C57BL/6-Tg(UBC-GFP)30Scha/J, B6.129(Cg)-Lepr^tm2(cre)Rck/J and B6.129S2-CD40lg^tm1lmx/J mice were purchased from The Jackson Laboratory. c-mpl^−/− mice (on a C57BL/6 background) were obtained from Genentech through a material transfer agreement.

Valet C., Magnen M., Qiu L., Cleary S.J., Wang K.M., Ranucci S., Grockowiak E., Boudra R., Conrad C., Seo Y., Calabrese D.R., Greenland J.R., Leavitt A.D., Passegué E., Méndez-Ferrer S., Swirski F.K, & Looney M.R. (2022). Sepsis promotes splenic production of a protective platelet pool with high CD40 ligand expression. The Journal of Clinical Investigation, 132(7), e153920.

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Genetic Mouse Models for Platelet Research

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