To determine the nanoparticle concentration in suspension, 100 μL of each nanoparticle solution was digested in 400 μL aqua regia (Nitric acid: hydrochloric acid, 3:1). Digested samples were diluted with 4.5 mL 1% nitric acid. Elemental concentration of gold in each sample was measured with the aid of an ELAN DRC II ICP-MS (Perkin Elmer, Inc., USA). The concentrations of spheres, cubes, RD, and rods (in ppm) were 2.29 × 103, 1.12 × 104, 1.18 × 104, and 1.44 × 103, respectively (Table 2 ).
Elan drc 2 icp ms
Manufactured by PerkinElmer
Sourced in United States, Canada
The ELAN DRC II ICP-MS is an inductively coupled plasma mass spectrometer (ICP-MS) designed for elemental analysis. It is capable of detecting and quantifying trace elements in a wide range of sample types.
Automatically generated - may contain errors
Lab products found in correlation
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (2 mentions)
Wiley mill, by Thomas Scientific (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Elan drcplus, by PerkinElmer (1 mentions)
X pert pro diffractometer, by Malvern Panalytical (1 mentions)
Bradford assay, by Bio-Rad (1 mentions)
Modular analyzer, by Roche (1 mentions)
0.45 μm nylon membrane, by Avantor (1 mentions)
Suprapur grade, by Merck Group (1 mentions)
Ethos d, by Milestone (1 mentions)
25 protocols using elan drc 2 icp ms
1
Quantifying Nanoparticle Concentrations via ICP-MS
2
Quantifying Cisplatin Accumulation in Biopsies
3
Measurement of Titanium and Iron in Blood
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Titanium (Ti) and iron (Fe) contents were measured from whole-blood samples based on Association of Analytical Communities 999.10 and determination by ICP-MS.26 (link)– 29 (link, link, link) Blood, nitric acid ( ), and hydrogen peroxide ( ) were added to the digestion vessel and digested using a microwave digester at four powers 250, 630, 500, and 0 W for 3, 5, 22, and 15 min, respectively. Then the samples were transported to and analyzed using a PerkinElmer ELAN DRC II ICP-MS. Afterward, the results were quantified by an external calibration method using standard solutions. Calibration curves were drawn from calibration blanks at five standard points with different concentrations.
4
ICP-MS Analysis of LigW Metal Content
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The metal content of LigW was determined
by inductively coupled plasma mass spectrometry (ICP-MS) with an Elan
DRC II ICP-MS instrument from PerkinElmer. Prior to ICP-MS analysis,
loosely bound metals were removed by passage through a PD-10 desalting
column equilibrated with 50 mM HEPES (pH 7.5) previously treated with
Chelex 100 ion-exchange resin. The protein sample was treated with
concentrated HNO3 for 15 min at 100 °C and then diluted
with distilled water to a final protein concentration of 1.0 μM
and 1% (v/v) HNO3. The activation of LigW by Mn2+, Zn2+, Co2+, or Fe2+ was analyzed
by the addition of these metal ions directly to buffered solutions
of the enzyme at pH 7.3 and the catalytic activity was monitored after
12 h of incubation at 4 °C. Iron was incubated anaerobically
under argon prior to measurement of catalytic activity. Metal-free
LigW was prepared by dialysis against 10 mM ο-phenanthroline at 4 °C in 50 mM HEPES, pH 6.5. A PD-10 column,
previously equilibrated with 50 mM HEPES at pH 7.3, was used to remove
the ο-phenanthroline prior to analysis by ICP-MS.
by inductively coupled plasma mass spectrometry (ICP-MS) with an Elan
DRC II ICP-MS instrument from PerkinElmer. Prior to ICP-MS analysis,
loosely bound metals were removed by passage through a PD-10 desalting
column equilibrated with 50 mM HEPES (pH 7.5) previously treated with
Chelex 100 ion-exchange resin. The protein sample was treated with
concentrated HNO3 for 15 min at 100 °C and then diluted
with distilled water to a final protein concentration of 1.0 μM
and 1% (v/v) HNO3. The activation of LigW by Mn2+, Zn2+, Co2+, or Fe2+ was analyzed
by the addition of these metal ions directly to buffered solutions
of the enzyme at pH 7.3 and the catalytic activity was monitored after
12 h of incubation at 4 °C. Iron was incubated anaerobically
under argon prior to measurement of catalytic activity. Metal-free
LigW was prepared by dialysis against 10 mM ο-phenanthroline at 4 °C in 50 mM HEPES, pH 6.5. A PD-10 column,
previously equilibrated with 50 mM HEPES at pH 7.3, was used to remove
the ο-phenanthroline prior to analysis by ICP-MS.
5
Quantifying Heavy Metal Uptake in Plants
6
Selenium Speciation in Wheat Seeds
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Seeds from control and low Se (10 kg Se/ha) treatments for both application methods and both Se forms were selected for analysis of organic Se species. Composite triplicate samples were ground to a fine powder, 250 mg of which was then mixed with 4 mL of nanopure water. Samples were digested with 10 mg of protease XIV (Streptomyces griseus) at 38°C for 90 min, after which they were centrifuged for 5 min (5000 g) and filtered through a 0.5 μm polytetrafluoroethylene (PTFE) membrane (Thavarajah et al., 2008 (link)). Selenium species were determined by high performance liquid chromatography (HPLC)/vapor generation/ICP-MS as previously described (Kuehnelt et al., 2006 (link)). A sample size of 30 μL was injected. The mobile phase flowed at 1 mL/min and was comprized of a phosphate buffer adjusted to pH 3.0 (5 mM NH4H2PO4 adjusted with H3PO4) with 1% NaBH4 and 5% HCl. Speciation of different Se forms was determined by ICP-MS (Elan DRCII, ICP-MS, Perkin Elmer Waltham, MA, USA) with a PE Series 200 HPLC fitted with micro pumps (Perkin Elmer, Waltham, MA, USA), a Waters Spherisorb 5 μm ODS2 4.6 × 250 mm column (Waters Corporation, Milford, MA, USA), and a Varian VGA-77 gas liquid separator (Agilent Technologies, Santa Clara, CA, USA; Kuehnelt et al., 2006 (link)). Standards (selenate, selenite, SeCys, SeMet, and Se-methylSeCys) were prepared at 40 ng Se mL-1.
7
Trace Metal Analysis of Chromatin
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Au, Ru and In standard solutions (1 g l−1 in 2% HCl, 2% HNO3 and 10% HCl, respectively) were purchased from CPI International (Amsterdam, The Netherlands). Hydrochloric acid (37%) at high-purity grade was purchased from Merck (Darmstadt, Germany).
Before determination of ruthenium and gold content, the chromatin extracts were digested with 400 μl of 37% Hydrochloric acid solution overnight at room temperature and adjusted with ultrapure water to a final volume of 4 ml. Indium was added as an internal standard at a concentration of 0.5 ppb. Determinations of total metal contents were achieved on an Elan DRC II ICP-MS instrument (Perkin Elmer, Switzerland) equipped with a Meinhard nebulizer and a cyclonic spray chamber. The ICP-MS instrument was tuned daily using a solution provided by the manufacturer containing 1 ppb each of Mg, In, Ce, Ba, Pb and U. External standards were prepared gravimetrically in an identical matrix to the samples (with regard to internal standard and Hydrochloric acid) with single element standards.
Before determination of ruthenium and gold content, the chromatin extracts were digested with 400 μl of 37% Hydrochloric acid solution overnight at room temperature and adjusted with ultrapure water to a final volume of 4 ml. Indium was added as an internal standard at a concentration of 0.5 ppb. Determinations of total metal contents were achieved on an Elan DRC II ICP-MS instrument (Perkin Elmer, Switzerland) equipped with a Meinhard nebulizer and a cyclonic spray chamber. The ICP-MS instrument was tuned daily using a solution provided by the manufacturer containing 1 ppb each of Mg, In, Ce, Ba, Pb and U. External standards were prepared gravimetrically in an identical matrix to the samples (with regard to internal standard and Hydrochloric acid) with single element standards.
8
Quantification of Intracellular Zinc Levels
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For quantification of the total Zn(II) quota, cells were grown in 5 ml LB medium in the presence or absence of 200 μM ZnCl2 to mid-log phase. For fractionation experiments, 25 ml of cells were grown in presence or absence of 200 μM Zn(II). All samples were prepared as described previously. Briefly, samples were washed once with buffer 1 (1X PBS buffer, 0.1 M EDTA) then twice with buffer 2 (1X chelex-treated PBS buffer). Cell pellets were resuspended in 400 μl buffer 3 (1X chelex-treated PBS buffer, 75 mM NaN3, 1% Triton X-100) and incubated at 37°C for 90 min for cell lysis. Lysed samples were centrifuged and subject to Bradford assay to quantify the total protein content. Then, samples were mixed with 600 μl buffer 4 (5% HNO3, 0.1% (v/v) Triton X-100) and heated in a 95°C sand bath for 30 min. Samples were centrifuged and supernatants were diluted in 1% HNO3. Levels of intracellular Zn were analyzed by Perkin-Elmer ELAN DRC II ICP-MS. Gallium was used as an internal standard. The total concentration of metal ions is expressed as μg ion per gram of protein. The data shown represent the average and standard deviation of three biological replicates.
9
Sequential Extraction of Metals in Ajka Red Mud
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ORP (as an indicator for Eh) and pH were measured using a Thermo Scientific Orion Dualstar pH/ISE benchtop meter (pH was calibrated daily at pH values of 4, 7 and 10; a new factory calibrated ORP electrode was used). Aqueous Cu and Ni concentrations were determined using a PerkinElmer Elan DRCII ICP-MS. DOC in end point solutions was determined by a multi N/C® 2100 analyser using thermocatalytic oxidation, MC-NDIR detection analysis. Sequential extractions were performed on triplicate Ajka red mud samples (collected from location K1 in Mayes et al. (2011 (link)) in December 2010) following an optimised Tessier procedure (Rauret et al. 1989 (link)) that partitioned Cu and Ni into five operationally defined fractions. Extractant pH was checked after each extraction stage and to ensure it conformed to protocol, and Cu and Ni concentrations were determined on an Optima 5300 DV ICP-OES.
10
Urinary Arsenic Speciation Analysis
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