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Ccr7 bv605

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The CCR7-BV605 is a fluorochrome-conjugated antibody that binds to the CCR7 receptor. CCR7 is a chemokine receptor that plays a role in the homing and migration of lymphocytes. The BV605 fluorochrome allows for detection and analysis of CCR7-expressing cells using flow cytometry.

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Lab products found in correlation

Ctla 4 apc, by Beckman Coulter (3 mentions) Igd pe, by Beckman Coulter (3 mentions) Cd127 cy5pe, by BioLegend (3 mentions) Ccr6 bv785, by Thermo Fisher Scientific (3 mentions) Cd27 bv605, by Thermo Fisher Scientific (3 mentions) Icos pac blue, by Thermo Fisher Scientific (3 mentions) Cd38 bv785, by Thermo Fisher Scientific (3 mentions) Live dead aqua, by Thermo Fisher Scientific (2 mentions) Cd3 h7apc sk7, by BD (2 mentions) Cd45ro trpe, by Beckman Coulter (2 mentions) Il 21 alexa 647, by Beckman Coulter (2 mentions) Cd154 pe, by Thermo Fisher Scientific (2 mentions) Cd20 apc h7, by Beckman Coulter (2 mentions) Igm pe cy5, by Beckman Coulter (2 mentions) Ki 67 alexa700, by Beckman Coulter (2 mentions) Ifng bv421 4, by BioLegend (2 mentions) Tnf α cy7pe, by Thermo Fisher Scientific (2 mentions) Il 2 alexa 700, by Thermo Fisher Scientific (2 mentions) Cd27 alexa594, by Thermo Fisher Scientific (2 mentions) Cd8 bv785, by Thermo Fisher Scientific (2 mentions)

4 protocols using ccr7 bv605

1

Flow Cytometry Immunophenotyping Panel

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Flow cytometry was performed using the following directly conjugated antibodies: (1) BD Biosciences: CD3 H7APC (SK7), CD45RA Cy7PE (L48), CD20 APC-H7 (2H7), CD45RO TRPE (UCHL), CTLA-4 APC (BNI3), IgD PE (IA6-2), IgG APC (G18-145), IgM PE-Cy5 (G20-127), IL-21 Alexa 647 (3A3-N2.1), and Ki-67 Alexa700 (150503); (2) Beckman Coulter: CD127 Cy5PE (R34.34); (3) BioLegend: PD-1 BV711 (EH12.2H7), IFNg BV421 (4S.B3), TNF-α CY7PE (MAb-11), IL-2 Alexa-700 (MQ1-17H12), CCR6 BV785 (G034E3), CD27 Alexa594 (IA4CD27), CD27 BV605 (O323), CCR7-BV605 (G043H7), CD8 BV785 (SK1), ICOS Pac Blue (C398.4A), CD19 Pac Blue (HIB19), CD38 BV785 (HIT2), and CD154 PE (24–31); (4) Invitrogen: CD4 Cy5.5PE (S3.5) and Aqua LIVE/DEAD® amine viability dye; and (5) eBioscience: CXCR5-FITC (MU5UBEE).
Paris R.M., Milagres L.G., Moysi E., Okulicz J.F., Agan B.K., Ganesan A., Petrovas C, & Koup R.A. (2017). Lower Baseline Germinal Center Activity and Preserved Th1 Immunity Are Associated With Hepatitis B Vaccine Response in Treated HIV Infection. Pathogens & Immunity, 2(1), 66-88.
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2

Comprehensive Immunophenotyping by Flow Cytometry

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Flow cytometry was performed using the following directly conjugated antibodies: (1) BD Biosciences: CD3 H7APC (SK7), CD45RA Cy7PE (L48), CD20 APC-H7 (2H7), CD45RO TRPE (UCHL), CTLA-4 APC (BNI3), IgD PE (IA6-2), IgG APC (G18-145), IgM PE-Cy5 (G20-127), IL-21 Alexa 647 (3A3-N2.1), and Ki-67 Alexa700 (150503); (2) Beckman Coulter: CD127 Cy5PE (R34.34); (3) BioLegend: PD-1 BV711 (EH12.2H7), IFNg BV421 (4S.B3), TNF-α CY7PE (MAb-11), IL-2 Alexa-700 (MQ1-17H12), CCR6 BV785 (G034E3), CD27 Alexa594 (IA4CD27), CD27 BV605 (O323), CCR7-BV605 (G043H7), CD8 BV785 (SK1), ICOS Pac Blue (C398.4A), CD19 Pac Blue (HIB19), CD38 BV785 (HIT2), and CD154 PE (24-31); (4) Invitrogen: CD4 Cy5.5PE (S3.5) and Aqua LIVE/DEAD® amine viability dye; and (5) eBioscience: CXCR5-FITC (MU5UBEE).
Paris R.M., Milagres L.G., Moysi E., Okulicz J.F., Agan B.K., Ganesan A., Petrovas C, & Koup R.A. (2017). Lower Baseline Germinal Center Activity and Preserved Th1 Immunity Are Associated With Hepatitis B Vaccine Response in Treated HIV Infection. Pathogens & Immunity, 2(1), 66-88.
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3

Flow Cytometry Immunophenotyping Panel

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Flow cytometry was performed using the following directly conjugated antibodies (clones): (1) BD Biosciences: CD3 H7APC (SK7 clone), CD45RA PE- Cy7 (L48), (2) BioLegend: CD3 BV650 (OKT3), IgM BV570 (MHM-88), PD-1 BV711 (EH12.2H7), CCR6 BV785 (G034E3), CD27 Alexa Fluor 594 (IA4CD27), CD27 BV605 (O323), CCR7 BV605 (G043H7), CCR7 APC (G043H7), CD8 BV650 (RPA-T8), ICOS Pac Blue (C398.4A), CD38 BV785 (HIT2); CXCR5 Alexa Fluor 488 (J252D4), CXCR3 PE (G025H7) (3) Invitrogen: CD4 PE-Cy5.5 (S3.5) and Aqua LIVE/DEAD® amine viability dye; (4) eBioscience: CXCR5 PE-CY7 (MU5UBEE); (5) Southern Biotech: IgD-PE; (6) Beckman Coulter: CD19 ECD (J3-119). The IgG (Alexa Fluor 680) and CD21 (PE-CY5.5) were conjugated in-house.
Milagres L., Silva G., Pereira-Manfro W., Frota A.C., Hofer C., Ferreira B., Barreto D., Figueredo M., Coelho B., Villela L., Petrovas C, & Koup R. (2018). Baseline Circulating Activated TFH and Tissue-Like Exhausted B Cells Negatively Correlate With Meningococcal C Conjugate Vaccine Induced Antibodies in HIV-Infected Individuals. Frontiers in Immunology, 9, 2500.
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4

Comprehensive Immune Phenotyping by Flow Cytometry

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Flow cytometry was performed using the following directly conjugated antibodies:
(1) BD Biosciences: CD3-H7APC (SK7), CD45RA-Cy7PE (L48), CTLA-4-APC (BNI3),
IFN-g-FITC (B27), CCR7-Alexa700 (150503), CCR5-FITC (2D7/CCR5), CCR4-PE (1G1) and
IL-2-PE (MQ1-17H12), Ki67-FITC (B56); (2) Beckman Coulter: CD27-Alexa680
(IA4CD27), CD127-Cy5PE (R34.34), CD160-PE (BY55), BTLA-PE (J168-540); (3)
BioLegend: PD-1-BV421 (EH12.2H7), 2B4-FITC (CD244, C1.7), IL-17a-Cy5.5PerCP
(BL168), CCR6-Alexa647 (or PE, TG7/CCR6), CD27 Alexa647 (O323), CCR7 BV605
(G043H7), CXCR5 BV421 (J252D4), and CD154-Cy5PE (24–31); (4) Invitrogen:
CD4-Cy5.5PE (S3.5), CD27-QD605 (CLB-27/1), CD8-QD800 (3B5). A biotinylated
anti-PD-1 antibody was obtained from R&D (BAF 1086) and streptavidin-Qdot
655 was obtained from Molecular Probes. Quantum dots and Aqua amine viability dye
were obtained from Invitrogen. CD27-Alexa 594, TNF-a Alexa 594, HLA-DR BV650, and
CD38-Alexa 680 were conjugated in-house.
Paris R.M., Petrovas C., Ferrando-Martinez S., Moysi E., Boswell K.L., Archer E., Yamamoto T., Ambrozak D., Casazza J.P., Haubrich R., Connors M., Ake J., Kim J.H, & Koup R.A. (2015). Selective Loss of Early Differentiated, Highly Functional PD1high CD4 T Cells with HIV Progression. PLoS ONE, 10(12), e0144767.
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