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Anti hsp90 antibody

Manufactured by Cell Signaling Technology
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The Anti-HSP90 antibody is a tool used in molecular biology research to detect and analyze the heat shock protein 90 (HSP90) in various samples. HSP90 is a chaperone protein involved in the folding and stabilization of other proteins. The antibody specifically recognizes and binds to HSP90, enabling researchers to study its expression, localization, and interactions within cells or tissues.

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Lab products found in correlation

P53 antibody, by Santa Cruz Biotechnology (2 mentions) Universal magnetic co ip kit, by Active Motif (2 mentions) Immpact novared peroxidase substrate, by Vector Laboratories (1 mentions) Vectastain elite abc kit, by Vector Laboratories (1 mentions) Anti actin antibody, by Santa Cruz Biotechnology (1 mentions) Anti pgc 1α antibody, by Merck Group (1 mentions) Anti gfp antibody, by Cell Signaling Technology (1 mentions) Anti ucp1 antibody, by Alpha Diagnostic (1 mentions) Anti proliferating cell nuclear antigen antibody, by Merck Group (1 mentions) Anti hsp90 antibody, by Abcam (1 mentions) Secondary horseradish peroxidase hrp conjugated anti mouse or anti rabbit igg antibodies, by Bio-Rad (1 mentions) U1260, by InvivoGen (1 mentions) 2 deoxyglucose, by Cayman Chemical (1 mentions) Enhanced chemiluminescence reaction, by Cytiva (1 mentions) Anti jev ns5, by GeneTex (1 mentions) Anti acetyl lysine, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

HSP90 (203 citations) Anti-HSP90 (93 citations) HSP90 antibody (8 citations) Anti-heat shock protein 90 (HSP90) antibody (2 citations) Polyclonal anti-HSP90 antibody (2 citations)

8 protocols using anti hsp90 antibody

1

Hsp90 Interaction with JEV NS5

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Lysate from mock- and JEV-infected cells was incubated with the anti-Hsp90 antibodies (Cell Signaling, Danvers, MA, USA) in a cool room overnight, followed by addition of protein A-Sepharose beads for an additional 4-h. The immunoprecipitate was collected after centrifugation, and analyzed using Western blotting with the anti-JEV NS5 (GeneTex, Inc.), anti-acetyl lysine (Cell Signaling), or anti-Hsp90 antibodies in a cool room overnight. After 4-h of incubation with horseradish peroxidase-conjugated secondary antibodies, the immune-reactive complexes were detected using enhanced chemiluminescence reaction (Amersham Pharmacia Biotech, Piscataway, NJ, USA).
Lu C.Y., Chang Y.C., Hua C.H., Chuang C., Huang S.H., Kung S.H., Hour M.J, & Lin C.W. (2017). Tubacin, an HDAC6 Selective Inhibitor, Reduces the Replication of the Japanese Encephalitis Virus via the Decrease of Viral RNA Synthesis. International Journal of Molecular Sciences, 18(5), 954.
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2

Immunohistochemical Detection of Hsp90

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Paraffin-embedded tissue sections were deparaffinized in xylene and rehydrated in a graded series of alcohol. Antigens were retrieved with 0.01 M citrate buffer (pH 6.0) by heating the samples in a microwave oven for 10 min. The tissue sections were then placed in 3% hydrogen peroxide for 5 min to inactivate endogenous peroxidase, and blocked for 30 min with normal horse serum (VECTASTAIN Elite ABC kit; Vector Laboratories, Burlingame, CA, USA). The primary antibody used for this study was anti-Hsp90 antibody (Cell Signaling). Pre-diluted primary antibodies were applied overnight at 4°C. The slides were then treated with biotinylated secondary antibody for 30 min at room temperature, followed by immPACT NovaRED Peroxidase substrate (Vector Laboratories) for 5 min at room temperature.
Kim J.G., Lee S.C., Kim O.H., Kim K.H., Song K.Y., Lee S.K., Choi B.J., Jeong W, & Kim S.J. (2017). HSP90 inhibitor 17-DMAG exerts anticancer effects against gastric cancer cells principally by altering oxidant-antioxidant balance. Oncotarget, 8(34), 56473-56489.
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3

Protein Expression Analysis by Western Blotting

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Western blotting (WB) was performed as previously described (Wang et al., 2013 (link)). Proteins were assessed with the following antibodies: anti-IRX3 antibody (Abcam, ab25703), anti-GFP antibody (Cell Signaling Technology, 2956s), anti-actin antibody (Santa Cruz Biotechnology, sc-8432), anti-UCP1 antibody (Alpha Diagnostic, ucp1-a), anti-HSP90 antibody (Cell Signaling Technology, 4877s), anti-PGC-1α antibody (Millipore, ab3242), anti-AP2 antibody (Cell Signaling Technology, 3544s), and horseradish peroxidase-conjugated (HRP)-linked secondary antibody (Cell Signaling Technology, 7076, 7074). The representative blotting bands were repeated in at least three mice. The results are representative of at least three independent experiments.
Zou Y., Lu P., Shi J., Liu W., Yang M., Zhao S., Chen N., Chen M., Sun Y., Gao A., Chen Q., Zhang Z., Ma Q., Ning T., Ying X., Jin J., Deng X., Shen B., Zhang Y., Yuan B., Kauderer S., Liu S., Hong J., Liu R., Ning G., Wang W., Gu W, & Wang J. (2017). IRX3 Promotes the Browning of White Adipocytes and Its Rare Variants are Associated with Human Obesity Risk. EBioMedicine, 24, 64-75.
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4

Co-immunoprecipitation of p53 and HSP90

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Co-immunoprecipitation was carried out using a Universal Magnetic Co-IP kit (Active Motif, Cat. # 54002). Small EVs from GOF TP53 HT29 and wt TP53 RKO cells were isolated as described above. Small EVs were lysed using kit lysis buffer, and 5 μg of p53 antibody (Santa Cruz, Cat. # sc-126) per sample was used for the pull-down of p53 and HSP90. The resulting proteins immunoprecipitated with p53 were then run on a denaturing gel, as described above. The presence of immunoprecipitated HSP90 in the same samples was determined by probing the blot with an anti-HSP90 antibody (Cell Signaling Technology, Cat. # 4877, 1:1000).
Ma S., McGuire M.H., Mangala L.S., Lee S., Stur E., Hu W., Bayraktar E., Villar-Prados A., Ivan C., Wu S.Y., Yokoi A., Dasari S.K., Jennings N.B., Liu J., Lopez-Berestein G., Ram P, & Sood A.K. (2021). Gain-of-function p53 protein transferred via small extracellular vesicles promotes conversion of fibroblasts to a cancer-associated phenotype. Cell reports, 34(6), 108726.
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5

Hsp90 Expression in ADPKD Cells

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ADPKD cells (1 × 104 cells/well) were plated in eight-well chamber slides, serum starved for 24 h, and treated for 48 h with 25 ng/mL EGF. Cells were fixed, permeabilized, and blocked with 1% BSA. The cells were then incubated for 1 h with anti-Hsp90 antibody (Cell Signaling), washed with PBS, and incubated for 1 h with FITC-conjugated secondary antibody. Slides were mounted with DAPI-containing mounting medium. Images were captured at ×60. Cultured metanephric kidneys were fixed in 4% paraformaldehyde, and frozen sections were cut. For staining, the sections were subjected to heat retrieval and incubated with anti-proliferating cell nuclear antigen (PCNA) antibody (Sigma) and with anti-mouse Texas red (Jackson Immunoresearch).
Sundar S.V., Zhou J.X., Magenheimer B.S., Reif G.A., Wallace D.P., Georg G.I., Jakkaraj S.R., Tash J.S., Yu A.S., Li X, & Calvet J.P. (2022). The lonidamine derivative H2-gamendazole reduces cyst formation in polycystic kidney disease. American Journal of Physiology - Renal Physiology, 323(4), F492-F506.
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6

Immunoprecipitation of Acetylated HSP90

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Total lysate (0.4 mg) from control and drug-treated cells was incubated overnight with 5 μl of anti-HSP90 antibody (Abcam, 13495) at 4 °C. Protein A/G magnetic beads (20 μl) were added to the mixture with incubation (gentle rotation) for 4 h at 4 °C. Immunoprecipitated samples were washed and subjected to Western blot analysis with anti-pan-acetyl-lysine antibody (Cell Signaling, 94415) and anti-HSP90 antibody.
Badal S., Her Y.F, & Maher LJ I.I.I. (2015). Nonantibiotic Effects of Fluoroquinolones in Mammalian Cells. The Journal of Biological Chemistry, 290(36), 22287-22297.
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7

Targeting 2DG and ERK in S. aureus Infection

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S. aureus (strain RN6390) was maintained in tryptic soy broth and agar plates (TSB/TSA; Sigma-Aldrich, St. Louis, MO). 2-Deoxy-glucose was purchased from Cayman Chemical (Ann Arbor, MI). For in vitro experiments cells were pretreated with 10mM of 2DG for 1 h followed by indicated infection times. For in vivo applications, the left eyes of each mouse was injected with 25 μg/eye (1μl volume) of 2DG, 14–16 h prior to or 6 h post bacterial infection. The dose of 2DG for intravitreal injection was chosen based on preliminary dose (10–100 μg/eye) titration. Contralateral eyes with sterile PBS injection were used as a controls. An ERK inhibitor, U1260, was purchased from InvivoGen (San Diego, CA) and cells were pretreated for 1 h with 10 μM of U1260. Antiphospho-ERK and ERK antibodies were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX). Anti-HSP90 antibody was purchased from Cell Signaling Technology (Beverly, MA). Secondary horseradish peroxidase (HRP)-conjugated anti-mouse or antirabbit IgG antibodies were purchased from Bio-Rad (Hercules, CA).
Francis R., Singh P.K., Singh S., Giri S, & Kumar A. (2020). Glycolytic Inhibitor 2-Deoxyglucose suppresses inflammatory response in Innate Immune Cells and Experimental Staphylococcal endophthalmitis. Experimental eye research, 197, 108079.
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8

Co-immunoprecipitation of p53 and HSP90

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Co-immunoprecipitation was carried out using a Universal Magnetic Co-IP kit (Active Motif, Cat. # 54002). Small EVs from GOF TP53 HT29 and wt TP53 RKO cells were isolated as described above. Small EVs were lysed using kit lysis buffer, and 5 μg of p53 antibody (Santa Cruz, Cat. # sc-126) per sample was used for the pull-down of p53 and HSP90. The resulting proteins immunoprecipitated with p53 were then run on a denaturing gel, as described above. The presence of immunoprecipitated HSP90 in the same samples was determined by probing the blot with an anti-HSP90 antibody (Cell Signaling Technology, Cat. # 4877, 1:1000).
Ma S., McGuire M.H., Mangala L.S., Lee S., Stur E., Hu W., Bayraktar E., Villar-Prados A., Ivan C., Wu S.Y., Yokoi A., Dasari S.K., Jennings N.B., Liu J., Lopez-Berestein G., Ram P, & Sood A.K. (2021). Gain-of-function p53 protein transferred via small extracellular vesicles promotes conversion of fibroblasts to a cancer-associated phenotype. Cell reports, 34(6), 108726.
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