Harvested organs were fixed in 4% paraformaldehyde (PFA) for 24 hours or IHC zinc fixative (BD Pharmingen) for anti-perforin stained tissues. Fixed tissues were then sucrose sedimented in 10%, 20% and 30% steps prior to embedding within optimum cutting temperature (O.C.T.) medium (Tissue Tek). Frozen sections of 5–7 μm thicknesses were cut and left to dry at ambient temperature. These tissues were then stained with primary antibodies: Cleaved Caspase 3 (Cell Signaling Technology), CD31 (Novus Biologicals INC), CD45 (Novus Biologicals INC), glial fibrillary acidic protein (GFAP) (Agilent Technologies INC), rabbit-neuronal marker (NeuN) (Cell Signaling Technology), guinea pig-NeuN (EMD Millipore Corporation), rat anti-perforin (Novus Biologicals INC) and anti-ZIKV rat serum 22 (link) after rehydration with PBS (2X for 10 minutes) and permeabilization with PBST (3X for 10 minutes) at RT overnight. Slides were washed and incubated with appropriate secondary prior to a final wash series and incubation with 4′,6-diamidino-2-phenylindole (DAPI) prior to mounting with SlowFade Diamond Antifade (Fisher Scientific Company LLC). Stained tissues were then analyzed by fluorescence microscopy (BX51; Olympus) or confocal microscopy (TCS SP2; Leica).
Rabbit neuronal marker neun
Manufactured by Cell Signaling Technology
Rabbit-neuronal marker (NeuN) is a laboratory reagent used to detect the presence of NeuN, a protein that is expressed in the nuclei of most neuronal cell types in the central nervous system. This product can be used in various experimental techniques, such as immunohistochemistry and Western blotting, to identify and quantify neuronal cells.
Automatically generated - may contain errors
Lab products found in correlation
Glial fibrillary acidic protein (gfap), by Agilent Technologies (2 mentions)
Guinea pig neun, by Merck Group (2 mentions)
Cleaved caspase 3, by Cell Signaling Technology (2 mentions)
Tcs sp2, by Leica (2 mentions)
Ihc zinc fixative, by BD (2 mentions)
Rat anti perforin, by Novus Biologicals (2 mentions)
2 protocols using rabbit neuronal marker neun
1
Multimodal Tissue Analysis of Organ Fixation
2
Multimodal Tissue Analysis of Organ Fixation
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Harvested organs were fixed in 4% paraformaldehyde (PFA) for 24 hours or IHC zinc fixative (BD Pharmingen) for anti-perforin stained tissues. Fixed tissues were then sucrose sedimented in 10%, 20% and 30% steps prior to embedding within optimum cutting temperature (O.C.T.) medium (Tissue Tek). Frozen sections of 5–7 μm thicknesses were cut and left to dry at ambient temperature. These tissues were then stained with primary antibodies: Cleaved Caspase 3 (Cell Signaling Technology), CD31 (Novus Biologicals INC), CD45 (Novus Biologicals INC), glial fibrillary acidic protein (GFAP) (Agilent Technologies INC), rabbit-neuronal marker (NeuN) (Cell Signaling Technology), guinea pig-NeuN (EMD Millipore Corporation), rat anti-perforin (Novus Biologicals INC) and anti-ZIKV rat serum 22 (link) after rehydration with PBS (2X for 10 minutes) and permeabilization with PBST (3X for 10 minutes) at RT overnight. Slides were washed and incubated with appropriate secondary prior to a final wash series and incubation with 4′,6-diamidino-2-phenylindole (DAPI) prior to mounting with SlowFade Diamond Antifade (Fisher Scientific Company LLC). Stained tissues were then analyzed by fluorescence microscopy (BX51; Olympus) or confocal microscopy (TCS SP2; Leica).
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