The frozen midbrains were stored at −80 °C and sectioned at the level of the SN, which can be clearly identified by its dark color15 (link). With a Microm HM550 cryostat (Thermo Scientific, Dreieich, Germany) combined with a fixed knife holder (Leica Biosystems, Nußloch GmbH, Nußloch, Germany), slices were cut at −18 °C object temperature and −20 °C chamber temperature. The 5, 10 or 20 μm thick sections were placed on 1.0 PEN membrane glass slides that were optimized for LMD (Carl Zeiss Microscopy GmbH, Göttingen, Germany).
1.0 pen membrane glass slides
Manufactured by Zeiss
Sourced in Germany
The 1.0 PEN-membrane glass slides are a specialized laboratory equipment designed for various scientific applications. They feature a polyethylene naphthalate (PEN) membrane adhered to a glass substrate, providing a versatile platform for various research and analysis techniques. The core function of these slides is to serve as a substrate for sample preparation and analysis, without making any interpretations or extrapolations about their intended use.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using 1.0 pen membrane glass slides
1
Cryosectioning of Midbrain Tissue
2
Post-Mortem Human SN Tissue Sampling
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Five µm thick slices of human post-mortem SN tissue were provided on 1.0 PEN-membrane glass slides (Carl Zeiss Microscopy GmbH, Göttingen, Germany) by the Navarrabiomed Biobank (Pamplona, Spain). The use of human brain tissue was approved by the ethics committee of the Ruhr-University Bochum, Germany (file number 4760-13), according to German regulations and guidelines. Ethical approval was obtained at 12 November 2013.
3
Post-mortem SN tissue slicing
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Flash frozen, human post mortem SN tissue slices of 5 µm thickness were provided on 1.0 PEN-membrane glass slides (Carl Zeiss Microscopy GmbH, Göttingen, Germany) by the Navarrabiomed Biobank (Pamplona, Spain). The use of human brain tissue was approved by the ethics committee of the Ruhr-University Bochum, Germany (file number 4760-13), according to German regulations and guidelines.
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