Volume-average diameter (nm) and polydispersity index (PDI) of NPs were analyzed by dynamic light scattering (DLS) using a NanoZetaSizer analyzer (Malvern Instruments Ltd., Malvern, UK). After the batch synthesis with NanoAssemblr®, native PLGA-NPs, 99mTc-polymer-(PLGA-NPs), and 99mTc-PVA-(PLGA-NPs) were loaded into the instrument and analyzed. All the batches were analyzed pre- and post-PD-10 purification step.
Briefly, 10 μL of each sample were suspended with 90 μL of ultrapure water and loaded in Sarstedt polystyrol/polystyrene cuvettes (10 × 10 × 45 mm) for the measurements performed at 25 °C. To study the surface charge of NPs, the zeta potential analysis was performed with the same instrument. In total, 20 μL of NPs were suspended with 980 μL of ultrapure water, sonicated, and loaded in Malvern folded capillary cells for measurements. All measurements were performed in triplicate and mean values ± standard deviation (SD) are reported.
Briefly, 10 μL of each sample were suspended with 90 μL of ultrapure water and loaded in Sarstedt polystyrol/polystyrene cuvettes (10 × 10 × 45 mm) for the measurements performed at 25 °C. To study the surface charge of NPs, the zeta potential analysis was performed with the same instrument. In total, 20 μL of NPs were suspended with 980 μL of ultrapure water, sonicated, and loaded in Malvern folded capillary cells for measurements. All measurements were performed in triplicate and mean values ± standard deviation (SD) are reported.