Linear ion trap (LIT) and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (Q TRAP), AB4500 Q TRAP UPLC/MS/MS System, equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion mode and controlled by Analyst 1.6.3 software (AB Sciex, Framingham, MA, USA). The ESI source operation parameters were as follows: ion source, turbo spray; source temperature 550 °C; ion spray voltage (IS) 5500 V (positive ion mode)/-4500 V (negative ion mode); ion source gas I (GSI), gas II(GSII), curtain gas (CUR) were set at 50, 60 and 25.0 psi, respectively; the collision-activated dissociation (CAD) was high. Instrument tuning and mass calibration were performed with 10 and 100 μmol/L polypropylene glycol solutions in QQQ and LIT modes, respectively. QQQ scans were acquired as multiple reaction monitoring (MRM) experiments with collision gas (nitrogen) set to medium. The declustering potential (DP) and collision energy (CE) for individual MRM transitions were done with further DP and CE optimization. A specific set of MRM transitions were monitored for each period according to the metabolites eluted within this period.
Turbo ion spray interface
Manufactured by AB Sciex
Sourced in United States, Canada
The Turbo Ion-Spray interface is a specialized component designed for AB Sciex mass spectrometry systems. It facilitates the efficient ionization and transfer of analytes from the liquid phase to the gas phase, enabling their detection and analysis by the mass spectrometer.
Automatically generated - may contain errors
Lab products found in correlation
Analyst 1, by AB Sciex (36 mentions)
Api 6500 q trap lc ms ms system, by AB Sciex (8 mentions)
Ab4500 q trap uplc ms ms system, by AB Sciex (7 mentions)
Api 4500 q trap uplc ms ms system, by AB Sciex (6 mentions)
6500 q trap, by Thermo Fisher Scientific (2 mentions)
Qtrap, by AB Sciex (2 mentions)
Qtrap 6500 system, by Waters Corporation (1 mentions)
Ab 6500 qtrap, by AB Sciex (1 mentions)
Acquity h class, by Waters Corporation (1 mentions)
Ultimate 3000 rs, by Thermo Fisher Scientific (1 mentions)
Vacutainer k2edta 5.4 mg tube, by BD (1 mentions)
Uplc hss t3 column, by Waters Corporation (1 mentions)
Multiquant 3, by AB Sciex (1 mentions)
Api 3000 mass spectrometer, by AB Sciex (1 mentions)
Biobasic scx, by Thermo Fisher Scientific (1 mentions)
Sil 20ac ht, by Shimadzu (1 mentions)
5500 qtrap triple quadrupole, by AB Sciex (1 mentions)
S1p d7, by Avanti Polar Lipids (1 mentions)
Analyst software, by AB Sciex (1 mentions)
Sunfire c18 column, by Waters Corporation (1 mentions)
43 protocols using turbo ion spray interface
1
Targeted Metabolomics Using UPLC-MS/MS
2
Metabolite Profiling of RAW264.7 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After 5 days of HA and β‐TCP treatment respectively, RAW264.7 cells were collected and centrifuged at 1200 r min−1 for 5 min at 4 °C, before washing them in cold PBS. Further centrifugation at 1200 r min−1 for 5 min at 4 °C was followed by vigorous vortexing with 1 mL of 80% cold methanol. The supernatant was collected and normalized to the protein concentration. Subsequently, the sample extracts were analyzed using an LC‐ESI‐MS/MS system (Waters ACQUITY H‐Class; MS, QTRAP 6500+ System). The AB 6500+ QTRAP LC‐MS/MS system was equipped with an ESI Turbo Ion‐Spray interface and can operate in both positive and negative ion modes according to Analyst 1.6 software (A B Sciex). Detection and determination of the relative abundances of the targeted energy metabolites were performed using MetWare based on the AB Sciex QTRAP 655 LC‐MS/MS platform.
3
Plasma endocannabinoid AEA measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Before and after each exercising condition, 2.5 mL of blood were collected into a BD Vacutainer K2EDTA 5.4 mg tube. This tube was immediately centrifuged for 10 min at 8009 g at 4 °C, the supernatant (plasma) was taken in aliquots of 200 μL. All samples were then frozen and stored at − 80 °C until analysis.
The endocannabinoid AEA levels were determined from 100 μl of plasma by liquid–liquid extraction. This was followed by liquid chromatography (Ultimate 3000RS, Dionex, CA, USA) and mass spectrometry using a 5,500 QTrap triple quadrupole/linear ion trap (QqQLIT) mass spectrometer equipped with a TurboIon-Spray interface (AB Sciex, Concord, ON, Canada) as described previously58 (link),59 (link).
The endocannabinoid AEA levels were determined from 100 μl of plasma by liquid–liquid extraction. This was followed by liquid chromatography (Ultimate 3000RS, Dionex, CA, USA) and mass spectrometry using a 5,500 QTrap triple quadrupole/linear ion trap (QqQLIT) mass spectrometer equipped with a TurboIon-Spray interface (AB Sciex, Concord, ON, Canada) as described previously58 (link),59 (link).
4
Quantitative Phytohormone Analysis by LC-MS/MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LC-MS/MS experiments were performed on a 5500 QTrap triple quadrupole/linear ion trap (QqLIT) mass spectrometer equipped with a TurboIon-Spray™ interface (AB Sciex, ON, Canada) coupled to a Shimadzu Exion UHPLC (Kyoto, Japan). Phytohormones were separated on a Poroshell 120 SB-C18 2.7 μm 2.1 × 150 mm ID column (Agilent Technologies, CA, USA) at 60 °C. Solvents were (A) water + 0.1% formic acid and (B) acetonitrile + 0.1% formic acid. The flow rate was 0.6 mL/min. The initial mobile phase of 2% B was held for 3 min, before ramping linearly to 16% B at 3.5 min, then to 100% B at 7 min and holding at 100% B for 1 min before resetting to the original conditions. The injection size was 10 μL. MS data were acquired in the negative mode, and positive mode (IAA and MeJA), using an MRM method with optimised Q1 and Q3 transitions for each analysed acidic phytohormone (Table S5 ). Other operating parameters were as follows: dwell time, 10 ms; ionspray voltage, −4500 V; ionspray voltage (IAA and MeJA), 4500 V; temperature, 600 °C; curtain gas, 45 psi; ion source gas 1, 60 psi; ion source gas 2, 60 psi. Data were analysed using Analyst version 1.7.2 and SciexOS version 2.0 software packages. Concentrations were calculated based on the peak area for the endogenous compounds relative to those determined for the internal standards.
5
Widely Targeted Metabolite Analysis by MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Mass spectrometry was according to the previous reported method for analyzing widely targeted metabolites (Chen et al., 2013 ). LIT and triple quadrupole (QQQ) scans were acquired using a triple quadrupole‐linear ion trap mass spectrometer (Applied Biosystems 6500 QTRAP). The MS/MS system was equipped with an ESI Turbo Ion Spray interface, operating in a positive ion mode and controlled by Analyst 1.6.3 software (AB Sciex, Waltham, MA, USA). The ESI source operation parameters were as follows: ion source, turbo spray; source temperature 500°C; ion spray voltage (IS) 5,500 V; ion source gas I (GSI), gas II (GSII), curtain gas (CUR) were set at 55, 60, and 25.0 psi, respectively; the collision gas (CAD) was high. Instrument tuning and mass calibration were performed with 10 and 100 μM polypropylene glycol solutions in QQQ and LIT modes, respectively. QQQ scans were acquired as MRM experiments with collision gas (nitrogen) set to 5 psi. DP and CE for individual MRM transitions was done with further DP and CE optimization. A specific set of MRM transitions were monitored for each period according to the metabolites eluted within this period.
6
Carvedilol Quantification by LC-MS/MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Carvedilol plasma concentrations were quantified by liquid chromatography–tandem mass spectrometry (LC-MS/MS) after protein precipitation using acetonitrile. The LC-MS system consisted of a Transcend LX2 platform (Thermo Fisher, Pittsburgh, PA, USA) and an AB Sciex API 5000 Triple Quadrupole mass spectrometer (AB Sciex, Concord, ON, Canada) operating in the positive ion mode using the TurboIonSpray interface (AB Sciex, Concord, ON, Canada). Carvedilol was separated using a Waters UPLC HSS T3 column (1.8 µm particle size, 50 × 2.1 mm internal diameter (I.D.)) and selected reaction monitoring of transition m/z 407.6–283.3 was used for quantitation (Waters Corporation, Milford, MA, USA). A calibration curve (1–5000 ng/mL) together with three sets of quality controls (5, 20, 2000 and 5000 ng/mL) were prepared and analyzed in the same manner as the study samples. Sciex MultiQuant 3.0.1 was used to process results.
7
Plasma Citrulline Kinetics Measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plasma citrulline levels were assessed at baseline and Weeks 4, 8, 16, and 24 in both studies. In addition, citrulline levels were assessed at Weeks 12 and 20 in the CL0600-004 study. Plasma samples were maintained at –70 °C until analysis. The analyte, L-citrulline, and internal standard D4 L-citrulline were extracted from 0.05 ml of human plasma by protein precipitation. Extracts were separated by reverse-phase chromatography on a Primesep 100 column (SIELC Technologies, Prospect Heights, IL, USA) using an isocratic gradient system of 0.2% formic acid in water and acetonitrile. Compounds were detected and quantified by tandem mass spectrometry in positive ion mode on an API 3000 mass spectrometer equipped with a TurboIonSpray interface (AB Sciex, Framingham, MA, USA). A generalized linear model was used for statistical analysis.
8
Quantitative mass spectrometry analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Linear ion trap (LIT) and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (Q-TRAP), API 4500 Q-TRAP UPLC/MS/MS system, equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion mode and controlled by Analyst 1.6.3 software (AB Sciex). Instrument tuning and mass calibration were performed with 10 and 100 μmol/L polypropylene glycol solutions in QQQ and LIT modes, respectively. Triple quadrupole scans were acquired as metabolic response modifier experiments with collision gas (nitrogen) set to 5 psi. Declustering potential (DP) and collision energy (CE) for individual multiple reactions monitoring (MRM) transitions were done with further DP and CE optimization. A specific set of MRM transitions were monitored for each period according to the metabolites eluted within this period.
9
Quantitative Mass Spectrometry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LIT and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (Q TRAP), AB4500 Q TRAP UPLC/MS/MS System, equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion modes and controlled by Analyst 1.6.3 software (AB Sciex, Toronto, ON, Canada). The ESI source operation parameters were as follows: ion source, turbo spray; source temperature 550 °C; ion spray voltage (IS) 5500 V (positive ion mode)/−4500 V (negative ion mode); ion source gas I (GSI), gas II (GSII), and curtain gas (CUR) were set at 50, 60, and 25.0 psi, respectively; the collision-activated dissociation (CAD) was high. Instrument tuning and mass calibration were performed with 10 and 100 μmol/L polypropylene glycol solutions in QQQ and LIT modes, respectively. QQQ scans were acquired as MRM experiments with collision gas (nitrogen) set to medium. DP and CE for individual MRM transitions were done with further DP and CE optimization. A specific set of MRM transitions were monitored for each period according to the metabolites eluted within this period.
10
Quantitative Mass Spectrometry Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Linear ion trap (LIT) and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (Q TRAP), API 4500 Q TRAP UPLC/MS/MS System, equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion mode and controlled by Analyst 1.6.3 software (AB Sciex). The main parameters were as follows: the temperature of electrospray ionization was set to 550 °C; the voltages of the ion sprays were 5500 V and −4500 V in positive and negative ion modes, respectively; the curtain gas (CUR) was 30 psi, and the collision-activated dissociation (CAD) was high. Each ion pair was scanned under the conditions of the optimum declustering potential (DP) and collision energy (CE) in the QQQ system [48 (link)]. Instrument tuning and mass calibration were performed with 10 and 100 μ mol/L polypropylene glycol solutions in QQQ and LIT modes, respectively. QQQ scans were acquired as MRM experiments with collision gas (nitrogen) set to 5 psi. DP and CE for individual MRM transitions were performed with further DP and CE optimization. A specific set of MRM transitions was monitored for each period according to the metabolites eluted within this period.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!