Dm400b microscope

Manufactured by Leica

Sourced in Germany

The DM400B is a high-quality microscope designed for versatile laboratory applications. It features a sturdy, ergonomic construction and advanced optics to provide clear, high-resolution images. The microscope is equipped with various illumination options and can accommodate a range of accessories to support diverse research and analysis tasks.

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Lab products found in correlation

Dfc420c camera, by Leica (2 mentions) Anti scl25a13, by Abcam (2 mentions) Ab115579, by Abcam (2 mentions) Dfc310 fx camera, by Leica (2 mentions) Dfc425c camera, by Leica (1 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions) Dako antifade mounting media, by Agilent Technologies (1 mentions) Giemsa reagent, by Merck Group (1 mentions) Prolong gold antifade with dapi, by Thermo Fisher Scientific (1 mentions) Dfc310 fx, by Leica (1 mentions) Vector vip substrate kit, by Vector Laboratories (1 mentions) Activated caspase 3, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

DM400B (35 citations) DM400B digital microscope (3 citations)

13 protocols using dm400b microscope

Quantifying Newborn Cells in Hippocampal Dentate Gyrus

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In order to determine the distribution of newborn cells within the hippocampal dentate gyrus (1 week and 6 week survival groups), we used two 40 μm sections per animal encompassing the middle of the dorsal dentate gyrus (−3.14 mm to −3.30 mm posterior to bregma). Outline tracings of the GCL were performed in StereoInvestigator software version 9.0 in a similar manner as the stereology protocol above (Figure 6). The thickness of the contour encompassing the GCL was kept at approximately 60 μm and was further sub-divided into 2 zones: GCL1 was adjacent to the subgranular zone (SGZ) and GCL2 was adjacent to the molecular layer (ML). Furthermore, two 30 μm contours encompassing the hilar zone adjacent to the SGZ were constructed: Hilus1 was most adjacent to the SGZ and Hilus 2 was furthest away. This method was adapted from previous reports.^{28 (link),29 (link)} The number of BrdU⁺ cells was determined in each of these zones by manual counting on a MBF Bioscience Leica DM400B microscope under high magnification of 40X/0.75 NA objective.

Ton S.T., Adamczyk N.S., Gerling J.P., Vaagenes I.C., Wu J.Y., Hsu K., O’Brien T.E., Tsai S.Y, & Kartje G.L. (2020). Dentate Gyrus Proliferative Responses After Traumatic Brain Injury and Binge Alcohol in Adult Rats. Neuroscience Insights, 15, 2633105520968904.

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Immunohistochemistry of Tumor Samples

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Six-micrometre sections were cut from 27 paraffin-embedded tumour samples (Supplementary Table 2) and mounted on Superfrost plus slides. The following antibodies and a standard procedure were used for immunohistochemistry40 (link) with Histogreen as a chromogen: anti-PC (1:100, ab115579, abcam), anti-SCL25A13 (1:50, abcam). Antigen retrieval was performed by boiling slides in Tris-EDTA buffer (pH9) for 45 min for PC and in citrate buffer (pH6) for SLC25A13. Images were acquired with a Leica DM400B microscope with Leica Application Suite software version 2.8.1, and a Leica DFC420C camera (Leica).

Lussey-Lepoutre C., Hollinshead K.E., Ludwig C., Menara M., Morin A., Castro-Vega L.J., Parker S.J., Janin M., Martinelli C., Ottolenghi C., Metallo C., Gimenez-Roqueplo A.P., Favier J, & Tennant D.A. (2015). Loss of succinate dehydrogenase activity results in dependency on pyruvate carboxylation for cellular anabolism. Nature Communications, 6, 8784.

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Microscopic Analysis of Tumor Samples

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All images were acquired on a Leica DM400B microscope (Leica, Wetzlar, Germany), at either 20× or 40× objectives. Images were acquired using a Leica DFC310 FX camera (Leica, Wetzlar, Germany) and LAS V3.8 software. Five images of at least 3 representative tumors were analyzed.

Witschen P.M., Chaffee T.S., Brady N.J., Huggins D.N., Knutson T.P., LaRue R.S., Munro S.A., Tiegs L., McCarthy J.B., Nelson A.C, & Schwertfeger K.L. (2020). Tumor Cell Associated Hyaluronan-CD44 Signaling Promotes Pro-Tumor Inflammation in Breast Cancer. Cancers, 12(5), 1325.

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Histological Analysis of Mouse Quadriceps

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The cross-sectional tissues of quadriceps femoris from each mouse were fixed with 4% paraformaldehyde (PH 7.4) for 24 h, processed, embedded in paraffin, and cut into 4-μm-thick sections. The sections were dewaxed and dehydrated. For histologic assessments, sections were stained with hematoxylin solution for 60 s, differentiated with 1% hydrochloric acid ethanol for 3 s, and stained with eosin-phloxine solution for 20 s. The tissues on each slide were added to a coverslip and imaged at magnification of 200 using a Leica DM400B microscope (Leica, Wetzlar, Germany).

Li J., Xie Q., Liu L., Cheng Y., Han Y., Chen X., Lin J., Li Z., Liu H., Zhang X., Chen H., Peng J, & Shen A. (2022). Swimming Attenuates Muscle Wasting and Mediates Multiple Signaling Pathways and Metabolites in CT-26 Bearing Mice. Frontiers in Molecular Biosciences, 8, 812681.

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Immunostaining Protocol for Cryosectioned Tissues

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Tissues were fixed in 4% paraformaldehyde at 4°C. Samples were prepared for embedding by soaking in 30% sucrose in PBS, and then quick-frozen in optimum cutting temperature (OCT) compound. Frozen sections (5–8 μm) were then blocked for 30 mins with 10% bovine serum albumin (BSA) and 2% goat serum followed by incubation with primary antibody for 12–16 hours. For immunostaining on sections from multi-colored mice, Alexa 647 or 750 conjugated antibody (1:1000) was used as a secondary stain for 1 hour (Invitrogen), and was visualized in the far-red channel (Cy5 or Cy7). Fluorescent images were taken with a Leica DM400B microscope (Leica Microsystems).

Corey D.M., Rinkevich Y, & Weissman I.L. (2015). Dynamic patterns of clonal evolution in tumor vasculature underlie alterations in lymphocyte-endothelial recognition to foster tumor immune escape. Cancer research, 76(6), 1348-1353.

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Imaging and Quantifying Border Cell Migration

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Flies were raised on standard yeast/cornmeal agar at 25°C. One day before dissection, flies were fed yeast paste. Ovaries were then extracted from 6–8 flies per each condition per slide and fixed in a 10% solution of paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) and phosphate-buffered solution for ∼30 min. They were then stained with Alexa568-phalloidin (Jackson ImmunoResearch, West Grove, PA) diluted 1:100 in PBST for at least 3 h at room temperature. Egg chambers were mounted on slides using Dako antifade mounting media (Agilent, Santa Clara, CA) and coverslips and sealed with clear nail polish. Slides were imaged by epifluorescence with a upright Leica DM400B microscope (Leica Microsystems, Buffalo Grove, IL) equipped with a Leica DFC425 C camera (Leica Microsystems, Buffalo Grove, IL) using 40×/0.65NA magnification. Egg chambers with border cells were staged and analyzed in FIJI. Quantification of border cell migration was carried out following the protocol established by Szafranski and Goode (2004) (link). Flies used in this study were UAS-ShotRNAi;;UAS-Dcr2 (Subramanian et al., 2003 (link)), UAS-Dcr2, slbo-GAL4, c306-GAL4 (Bloomington Drosophila Resource Center, Bloomington, IN), and UAS-Arp3RNAi (108951/KK; Vienna Drosophila Resource Center, Vienna, Austria).

Zhao A.J., Montes-Laing J., Perry W.M., Shiratori M., Merfeld E., Rogers S.L, & Applewhite D.A. (2022). The Drosophila spectraplakin Short stop regulates focal adhesion dynamics by cross-linking microtubules and actin. Molecular Biology of the Cell, 33(5), ar19.

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Tumor Imprint Cytology Protocol

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The tumor was surgically resected from mice; a tumor section was imprinted on the glass slide several times. Tumor smears were fixed with methanol during 7–10 min; staining was performed using Giemsa reagent (Sigma) at 1 : 10 dilution with deionized water during 30 min. An analysis was performed on a Leica DM 400B microscope.

Ruzanova V.S., Kirikovich S.S., Levites E.V., Proskurina A.S., Dolgova E.V., Ritter G.S., Efremov Y.R., Dubatolova T.D., Sysoev A.V., Koleno D.I., Ostanin A.A., Chernykh E.R, & Bogachev S.S. (2024). The Macrophage Activator GcMAF-RF Enhances the Antitumor Effect of Karanahan Technology through Induction of M2–M1 Macrophage Reprogramming. Journal of Immunology Research, 2024, 7484490.

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Quantifying Lung Metastasis via Microscopy

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All images were taken on a Leica DM400B microscope at either 20 × or 40 × objectives. Images were acquired using a Leica DFC310 FX camera and LAS V3.8 software and processed in ImageJ. 3 images per lung were analyzed for metastasis.

Jesser E.A., Brady N.J., Huggins D.N., Witschen P.M., O’Connor C.H, & Schwertfeger K.L. (2021). STAT5 is activated in macrophages by breast cancer cell-derived factors and regulates macrophage function in the tumor microenvironment. Breast Cancer Research : BCR, 23, 104.

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Visualizing Endocytosis of HA in Cells

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Cells were incubated with TexasRed labeled LMW HA (Cat #H-025R, Echelon Biosciences) in fresh medium for 45 min at 37 °C in dark, then washed with PBS and fixed with 2% PFA. Cells were subsequently permeabilized with 2% Triton X-100 for 30 minutes, stained with Phalloidin (1:500 dilution, Thermo Fisher Scientific, MA, USA, Cat # 501720934,) for 1 hour at RT, and mounted with ProLong^™ gold antifade with DAPI (Invitrogen, OR, USA, Cat.# P36931). Images were acquired on a on a Leica DM400B microscope (Wetzlar, Germany) at 400X.

Wheeler J.M., Beck N.E., Kim H.C., Tomlinson I.P., Mortensen N.J, & Bodmer W.F. (1999). Mechanisms of inactivation of mismatch repair genes in human colorectal cancer cell lines: The predominant role of hMLH1. Proceedings of the National Academy of Sciences of the United States of America, 96(18), 10296-10301.

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Quantifying Catecholaminergic Varicosities in Prefrontal Cortex

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Cg1, PrL, and IL subregions of the PFC were delineated according to plates spanning 14–18 of the mouse brain atlas¹¹⁰ and contours were traced at 5X magnification using a Leica DM400B microscope along the dense TH-positive innervation of PFC layers V-VI. An unbiased counting frame (50 ×50 μm) was superimposed on each contour and counts were made at regular predetermined intervals (x = 175 μm, y = 175 μm) from a random start point. TH positive and eYFP positive varicosities were counted at 100X magnification on 5 sections contained within the rostrocaudal borders of our region of interest (Plates 14–18; 1:4 series). A guard zone of 4 μm was used and the optical disector height was set to 10 μm.

Reynolds L.M., Hernandez G., MacGowan D., Popescu C., Nouel D., Cuesta S., Burke S., Savell K.E., Zhao J., Restrepo-Lozano J.M., Giroux M., Israel S., Orsini T., He S., Wodzinski M., Avramescu R.G., Pokinko M., Epelbaum J.G., Niu Z., Pantoja-Urbán A.H., Trudeau L.É., Kolb B., Day J.J, & Flores C. (2023). Amphetamine disrupts dopamine axon growth in adolescence by a sex-specific mechanism in mice. Nature Communications, 14, 4035.

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