Ifn β enzyme linked immunosorbent assay elisa kit

Murine Embryonic Fibroblast (MEF) cell lines were kindly provided by Dr. Genhong Cheng (University of California, Los Angeles). U937 human monocytes and Human Embryonic Kidney (HEK) 293T cells were obtained from American Type Culture Collection (ATCC). Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Corning) (MEF and HEK 293T) or Roswell Park Memorial Institute 1640 (RPMI 1640) (Corning) (U937) supplemented with fetal bovine serum (Gibco) (10%) and penicillin/streptomycin (Gibco) (1%) in 5% CO₂ at 37°C. Poly (I:C) (HMW) and 2’3’-cGAMP were purchased from Invivogen. Calf-thymus DNA and oligos for ISD generation were synthesized by Invitrogen. Recombinant murine IFN-β was purchased from MedChemExpress. An IFN-β enzyme linked immunosorbent assay (ELISA) kit were from PBL Assay Science. Anti-Flag M2 antibody was purchased from Sigma-Aldrich. Other primary antibodies used in this study were GFP, HA, Myc, VSV-G, HSV-1 ICP4 (Santa Cruz Biotechnology); TBK1, p-TBK1, IRF3, p-IRF3, STAT1, p-STAT1 (Cell Signaling Technology); TRIM28, β-Tubulin, GAPDH, RSAD2, control IgG (Proteintech); and K63 ubiquitin (Millipore). Secondary anti-rabbit, anti-mouse, or anti-rabbit Trueblot antibodies conjugated to HRP were purchased from Southern Biotech and Rockland Immunochemicals, respectively.