BJAB (EBV-negative Burkitt's lymphoma cell line), Raji (EBV-positive Burkitt's lymphoma cell line), B95.8 (EBV-transformed tamarin (Saguinus oedipus) cell line) cells and HONE-1 (human nasopharyngeal carcinoma cell line) were cultured in RPMI 1640 supplemented with 10% fetal bovine serum (FBS). HEK293 cells were cultured in Dulbecco’s modified Eagle medium supplemented with 10% FBS. All cells were confirmed to be Mycoplasma negative before culture (TaKaRa) and were authenticated by the short tandem repeat Multiamplification Kit (Goldeneye DNA ID system 20A, Peoplespot) every six months. PBMCs were isolated from whole blood of healthy donors by Ficoll centrifugation. Primary B cells were isolated from PBMCs by MojoSort human Pan B cell Isolation Kits (480081, BioLegend) and were cultured in RPMI 1640 medium supplemented with 10% FBS. All cell lines were grown in a humidified incubator at 37 °C with 5% CO2 and obtained from American Type Culture Collection. CHX (M4879) were purchased from AbMole BioScience. STM2457 (HY-134836) and MG132 (HY-13259) was purchased from MedChemExpress.
Chx m4879
Manufactured by AbMole
Sourced in United States
The CHX (M4879) is a laboratory equipment designed for general use. It functions as a centrifuge, a device used to separate components of a liquid mixture based on their density differences by the application of centrifugal force.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using chx m4879
1
Cell Culture and Authentication Protocol
2
Antibodies and Reagents for Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibodies against PFKP (sc-514824), PKM (sc-365684), LDHA (sc-137243), PD-L1 (sc-293425), HLA-E (sc-51621), and ubiquitin (sc-8017) were purchased from Santa Cruz Biotechnology (SantaCruz, CA, USA). The IgG isotypes (#3900) were purchased from Cell Signaling Technology (Beverly, MA, USA). GPR81 antibody (A20321) was purchased from Abclonal (Wuhan, China). PD-L1 (ab213524) and HLA-E (ab300553) antibodies were purchased from Abcam (Cambridge, UK). β-actin antibody (E021020-01) was purchased from Earthox (Burlingame, CA, USA). The siRNAs targeting GPR81, PKM, and LDHA were synthesized by GenePharma (Shanghai, China). CHX (M4879) was purchased from Abmole (Houston, TX, USA). Recombinant human IL-6 (200-06) was purchased from Peprotech (Cranbury, NJ, USA). MG-132 (S2619), Sodium oxamate (S6871), Bucladesine (S7858), and forskolin (S2449) were purchased from Selleck (Houston, TX, USA).
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