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33 gauge blunt needle

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The 33-gauge blunt needle is a laboratory equipment designed for precise liquid handling tasks. It features a small 33-gauge diameter and a blunt tip, which helps minimize sample loss and damage during transfers.

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Lab products found in correlation

Nanofil syringe, by World Precision Instruments (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Operating microscope, by Leica camera (1 mentions)

2 protocols using 33 gauge blunt needle

1

In Vivo Gene Editing in Murine Eyes

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Three-week-old mice (juvenile mice) or 6-month-old mice (adult mice) were anesthetized. The mice then received a subretinal injection of a 1:1 (v/v) mixture of RNP and Lipofectamine 2000 (catalog no. 11668019, Thermo Fisher Scientific) in one eye using a customized NanoFil syringe with a 33-gauge blunt needle (World Precision Instruments), as previously described (48 ). Each dose included 12.54 μg of NG-ABEmax and 5.76 μg of the appropriate sgRNA. The total volume was 3 μl per eye.
Jang H.K., Jo D.H., Lee S.N., Cho C.S., Jeong Y.K., Jung Y., Yu J., Kim J.H., Woo J.S, & Bae S. (2021). High-purity production and precise editing of DNA base editing ribonucleoproteins. Science Advances, 7(35), eabg2661.
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2

Subretinal AAV-Mediated CRISPR/Cas9 Gene Therapy

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After deep anesthesia, AAV9-SpCas9 and AAV9-TS4 sgRNA-Rpe65-donor (a total of 2 × 1010 or 2 × 1011 vg in 1 μl of phosphate-buffered saline) were mixed and injected into the subretinal space of the mouse eye through the vitreous cavity using a customized Nanofil syringe with a 33-gauge blunt needle (World Precision Instrument) under an operating microscope (Leica), as previously described (35 (link)).
Jo D.H., Song D.W., Cho C.S., Kim U.G., Lee K.J., Lee K., Park S.W., Kim D., Kim J.H., Kim J.S., Kim S., Kim J.H, & Lee J.M. (2019). CRISPR-Cas9–mediated therapeutic editing of Rpe65 ameliorates the disease phenotypes in a mouse model of Leber congenital amaurosis. Science Advances, 5(10), eaax1210.
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