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High density nickel resin

Manufactured by GoldBio

High-density nickel resin is a laboratory equipment product used for various applications. It is a solid-phase resin composed of high-density nickel particles. The primary function of this product is to serve as a specialized material for various laboratory processes and experiments.

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2 protocols using high density nickel resin

1

Radiolabeled Fatty Acid Metabolism Assay

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Fatty acid-free BSA, myristic acid, oleic acid, palmitic acid, and 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB) were purchased from Sigma-Aldrich (St. Louis, MO). Antibiotics, high-density nickel resin, and IPTG were purchased from GoldBio (St. Louis, MO). [1-14C]acetate (55 mCi/mmol) and [1-14C]oleate (56.3 mCi/mmol) were purchased from PerkinElmer (Waltham, MA). Bacteria media supplies were purchased from BD Medical Technologies (Franklin Lakes, NJ). All chemicals and solvents were reagent grade or better.
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2

Radiolabeled Fatty Acid Synthesis

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[1-14C]Palmitic acid (56.7 mCi/mmol) was from PerkinElmer Life Sciences. Antibiotics, high density nickel resin, and isopropyl β-d-thiogalactopyranoside were from GoldBio. All other reagents were from Sigma unless otherwise indicated.
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