Jsm 1200 ex 2 transmission

TEM was used to observe the morphology and mesostructure of the NPs. The samples were prepared and dried by dropping them onto a carbon-coated copper grid and then observing them on a JEOL JSM-1200 EX II transmission electron microscope operated at 100 kV. Total surface areas and average pore diameters were determined by measuring the nitrogen adsorption–desorption isotherm on a Micrometerics ASAP 2010 apparatus and calculation by the BET and Barrett–Joyner–Halenda (BJH) methods. The hydrodynamic diameters of the NPs in water, PBS, serum-free, and culture medium supplemented with 10% fetal bovine serum (FBS) were assessed using DLS on a Malvern Zetasizer Nano ZS instrument. Zeta potential measurements were performed on a Malvern Zetasizer Nano ZS instrument by determining the electrophoretic mobility and applying Henry’s equation.