Total RNA was extracted from each cell pellet using the RNA assay Minikit (Quiagen, Hilden, Germany) with DNAse I treatment. The amount of extracted RNA was quantified by measuring the absorbance at 260 nm. The purity of RNA was checked by measuring the ratio of the absorbance at 260 and 280 nm, where a ratio ranging from 1.8 to 2.0 was taken to be pure.
Minikit
Manufactured by Qiagen
Sourced in Germany
The Minikit is a compact, easy-to-use laboratory equipment designed for DNA or RNA extraction and purification. It provides a reliable and efficient solution for small-scale sample processing in research or diagnostic settings.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using minikit
1
RNA Extraction and Quantification
2
Total RNA Extraction from Differentiated Cells
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The RNA assay Minikit (Quiagen, Hilden, Germany) with DNAse I treatment was used to extract total RNA, as previously reported [22 (link)]. RNA obtained from differentiated MSCs, SW1353, and CPCs was then quantified spectrophotometrically. The RNA preparation was considered pure when the 260/280 nm absorbance ratio was in the 1.8 to 2.0 range. For gene expression studies related to CPCs, RNA samples were pooled across the six subjects in order to reduce the bias that each individual might introduce.
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