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Pnu 120596

Manufactured by Selleck Chemicals
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Sourced in United States

PNU-120596 is a laboratory reagent used for various experimental applications. It functions as a selective positive allosteric modulator of the α7 nicotinic acetylcholine receptor. The core purpose of this product is to facilitate research and investigation in the fields related to the α7 nicotinic acetylcholine receptor and its associated biological processes.

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Lab products found in correlation

Acetylcholine chloride, by Merck Group (1 mentions) Methyllycaconitine mla, by Abcam (1 mentions)

4 protocols using pnu 120596

1

Pharmacological Evaluation of GTS-21 and PNU-120596

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GTS-21 was made in house at the University of Florida as described previously (39 (link)). PNU-120596 was purchased from Selleckchem (Houston, TX). Drugs were dissolved in dimethyl sulfoxide (DMSO; vehicle) to create an 80 mM stock solution that was kept frozen at −30°C until the day of experiments. The amount of DMSO injected in each animal did not exceed 0.5 ml/kg. Analytical data for GTS-21 including melting point have been reported previously (39 (link)). Purity was ≥98% as determined by proton NMR analysis. In the current study, GTS-21 was administered subcutaneously at a dose of 15 mg/kg/day and PNU-120596 was administered subcutaneously at a dose of 10 mg/kg/day. Drugs administered at 4–20 mg/kg/day were found effective in other studies (37 (link), 40 (link)–42 (link)).
Morales J.Y., Young-Stubbs C.M., Shimoura C.G., Kem W.R., Uteshev V.V, & Mathis K.W. (2021). Systemic Administration of α7-Nicotinic Acetylcholine Receptor Ligands Does Not Improve Renal Injury or Behavior in Mice With Advanced Systemic Lupus Erythematosus. Frontiers in Medicine, 8, 642960.
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2

Acetylcholine and PNU-120596 Solution

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Acetylcholine chloride (Sigma-Aldrich) was dissolved to 1 M stock solutions in ND96 buffer. PNU-120596 (Selleckchem) was dissolved in DMSO to 150 mM stock solutions. Further dilution was performed to make a 10 μM and 0.1% v/v DMSO solution for experimentation.
Marotta C.B., Lester H.A, & Dougherty D.A. (2015). An unaltered orthosteric site and a network of long-range allosteric interactions for PNU-120596 in α7 nicotinic acetylcholine receptors. Chemistry & biology, 22(8), 1063-1073.
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3

Neurochemical Reagents for Cell Studies

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PNU-120596 was purchased from Selleck Chemicals (Houston, TX, USA). Methyllycaconitine (MLA) was purchased from Abcam Biochemicals (Cambridge, MA, USA). KN92/KN93 were purchased from Cayman Chemical (Ann Arbor, MI, USA). Other chemicals were purchased from ThermoFischer Scientific (Waltham, MA, USA).
Sun F., Johnson S.R., Jin K, & Uteshev V.V. (2016). Boosting Endogenous Resistance of Brain to Ischemia. Molecular neurobiology, 54(3), 2045-2059.
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4

Therapeutic Potential of PNU 120596

Cited 2 times
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PNU120596 was purchased from Selleckchem (Houston, TX) and dissolved in dimethyl sulfoxide (DMSO; vehicle) to create a stock solution (10–80 mM) that was kept frozen at −30°C until the day of experiments. PNU 120596 was administered at doses found effective in our previous studies where only single acute treatments were used [5 (link),9 ,14 (link),15 (link)], The main goal of this study is to determine therapeutic utility of sub-chronic PNU 120596 treatments. The amount of DMSO injected in each animal did not exceed 0.5 ml/kg. I.V. injections were given via a tail vein while s.c. injections were given in the animal back or thighs.
Gaidhani N, & Uteshev V.V. (2018). Treatment duration affects cytoprotective efficacy of positive allosteric modulation of α7nAChRs after focal ischemia in rats.. Pharmacological research, 136, 121-132.
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