To quantitate aortic remodeling, IMT was measured using hematoxylin and eosin staining; elastin breaks were counted via Elastin Verhoeff's–Van Gieson staining; and collagen deposition was evaluated with Masson's trichrome staining. Hematoxylin and eosin, Elastin Verhoeff's–Van Gieson, and Masson trichrome staining were performed using MasterTech stain kits (StatLab, McKinney, TX). Staining of aortic walls was performed as described in previous studies.18 In brief, aortic paraffin sections (5 μm in thickness) were used for immunostaining with antiproliferating cellular nuclear antigen, tissue necrosis factor‐alpha (TNF‐α), intercellular adhesion molecule 1 (ICAM1), vascular cell adhesion molecule 1 (VCAM1), and collagen type I antibodies. Details of primary antibodies used are listed in Table 1 . The ratios of target immunohistochemical staining positive area to the total tissue or cell area were determined via a computer‐imaging program according to the instruction provided by manufacture (MetaMorph Imaging System; Molecular Devices, San Jose, CA).
Mastertech stain kits
Manufactured by StatLab
The MasterTech stain kits are a series of solutions designed for staining and preparation of biological samples for microscopic examination. The kits contain a variety of stains, buffers, and other reagents required for the staining process. The core function of the MasterTech stain kits is to provide a standardized and reliable staining procedure for various cell types and tissue samples.
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Lab products found in correlation
2 protocols using mastertech stain kits
1
Quantifying Aortic Remodeling Markers
2
Aortic Remodeling Quantification Protocols
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To quantitate aortic remodeling, IMT was measured using hematoxylin and eosin (H&E) staining; elastin breaks were counted via Elastin Verhoeff's-Van Gieson (EVG) staining; and collagen deposition was evaluated with Masson's trichrome staining. H&E, EVG, and Masson trichrome staining were performed using MasterTech stain kits (StatLab, McKinney, TX).
Staining of aortic walls was performed as described in previous studies. 18 In brief, aortic paraffin sections (5 µm in thickness) were utilized for immunostaining with anti-intercellular adhesion molecule 1 (ICAM1), vascular cell adhesion molecule 1 (VCAM1), and collagen type I antibodies. Details of primary antibodies used are listed in Table 1. The ratios of target immunohistochemical staining positive area to the total tissue or cell area were determined via a computer-imaging program according to the instruction provided by manufacture (MetaMorph Imaging System, Molecular Devices, San Jose, CA).
Staining of aortic walls was performed as described in previous studies. 18 In brief, aortic paraffin sections (5 µm in thickness) were utilized for immunostaining with anti-intercellular adhesion molecule 1 (ICAM1), vascular cell adhesion molecule 1 (VCAM1), and collagen type I antibodies. Details of primary antibodies used are listed in Table 1. The ratios of target immunohistochemical staining positive area to the total tissue or cell area were determined via a computer-imaging program according to the instruction provided by manufacture (MetaMorph Imaging System, Molecular Devices, San Jose, CA).
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