Anti set7

Manufactured by Abcam

Anti-SET7 is a primary antibody that recognizes the SET7/9 protein, a histone methyltransferase responsible for monomethylation of lysine 4 on histone H3 (H3K4me1). This antibody can be used in various applications such as western blotting, immunoprecipitation, and immunohistochemistry to detect and study the SET7/9 protein.

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Lab products found in correlation

2 protocols using anti set7

Antibody Procurement and Utilization

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Antibodies including anti-EGLN1 (#4835), anti-HIF1α (#36169), anti-hydroxy-HIF1α (Pro564) (#3434), anti-SET7 (#2825), and normal rabbit immunoglobulin G (#2729) were purchased from Cell Signaling Technology. Anti-β-actin (#AC026) was purchased from ABclonal. Anti-Flag (#F1804) was purchased from Sigma. Anti-HA (#901515) was purchased from Covance. Anti-Myc (#SC-40) and Anti-GAPDH (#SC-47724) antibody was purchased from Santa Cruz Biotechnology. anti-SET7 (#ab124708) was purchased from Abcam. MG-132 (#474790) was purchased from Sigma. Cycloheximide (#HY-12320) was purchased from MCE.

Tang J., Deng H., Wang Z., Zha H., Liao Q., Zhu C., Chen X., Sun X., Jia S., Ouyang G., Liu X, & Xiao W. (2022). EGLN1 prolyl hydroxylation of hypoxia-induced transcription factor HIF1α is repressed by SET7-catalyzed lysine methylation. The Journal of Biological Chemistry, 298(6), 101961.

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Antibody Reagents for Protein Analysis

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Antibodies including anti-OTUB1 (#3783), anti-SET7 (#2825), anti-His (#9991), anti-Histone H3 (#4499), anti-UBC13 (#6999), anti-HA (#3724), anti-Ubiquitin (#3936), and normal rabbit IgG (#2729) were purchased from Cell Signaling Technology. anti-OTUB1 (#ab270959) and anti-SET7 (#ab124708) antibodies were purchased from Abcam. Anti-β-actin (#AC026) antibody was purchased from ABclonal. Anti-Flag (#F1804) antibody was purchased from Sigma. anti-HA (#901515) antibody was purchased from Covance. Anti-Myc (#SC-40) antibody was purchased from Santa Cruz Biotechnology. Anti-α-tubulin (#62204) and Alexa Fluor 488 goat anti-rabbit IgG (#A11008) were purchased from Thermo Fisher Scientific. Erastin (#HY-15763) was purchased from MCE. TBH solution (#B802372) was purchased from Macklin. CHX (#HY-12320) was purchased from MCE.

Deng H., Jia S., Tang J., Rong F., Xu C., Chen X., Wang Z., Zhu C., Sun X., Liao Q., Liu W., Li W., Xiao W, & Liu X. (2023). SET7 methylates the deubiquitinase OTUB1 at Lys 122 to impair its binding to E2 enzyme UBC13 and relieve its suppressive role on ferroptosis. The Journal of Biological Chemistry, 299(4), 103054.

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