Benzodiazocine 10 carboxylic acid

Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin, and streptomycin were purchased from PAN-Biotech GmbH (Am Gewerbepark, Aidenbach). VEGF, (10 ng/mL), bradykinin (BK; 1 μM), diethylenetriamine NONOate (DEAN; 10 μM), 1H-[1,2,4]-oxidiazolo[4,3a]quinozalin-1-one (ODQ; 10 μM), guanosine-3′5-monophosphate 8-bromo-sodium salt (8-Br-cGMP; 50 μM), sildenafil citrate (SC; 1 μM), L-nitro-L-arginine-methyl ester (L-NAME; 1 mM), 2,3,9,10,11,12-hexahydro-10R-methoxy-2,9-dimethyl-1-oxo-9S,12R-epoxy-1H-diindolo[1,2,3-fg:3′, 2′,1′-kl]pyrrolo[3,4-i] [1, 6] benzodiazocine-10-carboxylic acid, methyl ester (KT5823; 1 μM), N_ω-nitro-D-arginine methyl ester hydrochloride (D-NAME; 1 mM), diaminorhodamine-4M-AM (DAR-4M-AM; 5 μM) were purchased from Sigma Chemical Co., St. Louis, MO, USA. Acetylcholine (Ach; 5 μM; Himedia), 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide (cPTIO; 10 μM; Calbiochem), myosin light chain kinase inhibitor (MLCKi; 30 μM; Santa Cruz, USA), phalloidin and DAPI were purchased from Invitrogen, USA. Caveolin-1 scaffolding domain peptide (CSP; 14 μM) was purchased from Merck Millipore, Germany. DETA NONOate (DETA) was purchased from Cayman Chemical, USA. All other chemicals used in this study were of the reagent grade and were obtained commercially.

The following materials were obtained from the sources indicated: acetic acid from Mallinckrodt Baker, S.A (Mexico City, Mexico); glybenclamide, KT5823 (2,3,9,10,11,12-hexahydro-10R-methoxy-2,9-dimethyl-1-oxo-9S,12R-epoxy-1H diindolo [1,2,3-fg:3′,2′, 1′-kl] pyrrol [3,4-i][1 (link),6 (link)]benzodiazocine-10-carboxylic acid, methyl ester) and L-cysteine were obtained from Sigma-Aldrich (St. Louis, MO, USA). ODQ (1H-[1 (link),2 (link),4 (link)]oxadiazolo[4,3-a]quinoxalin-1-one) was obtained from Calbiochem (San Diego, CA, USA). Angeli’s salt (Na₂N₂O₃, sodium trioxodinitrate) was synthesized and utilized as previously described [32 ] The stability of stock solutions prepared in 10 mM NaOH and stored at −20°C, was determined from the extinction coefficients at 250 nm (ε of 8000 M⁻¹ cm⁻¹ for Angeli’s salt) [33 (link)]. The selected doses of drugs were chosen based on pilot studies and previous data of our laboratory. We detected that the doses of drugs used do not alter the nociceptive response per se, and inhibit the respective standard stimulus [16 (link), 18 (link), 34 (link), 35 (link),36 (link), 37 (link)]