Fresh tissues for phenotypic assays or collected primary GC cells for functional assays to test separation purity were fixed, dehydrated and paraffin embedded. Paraffin sections were dewaxed and rehydrated using a routine protocol [22 (link)]. The cells underwent antigen repair, neutralization of endogenous catalases, serum blocking, incubation with anti-CD137 rabbit mAb antibody (1:100, CST, USA), anti-Foxp3 rabbit mAb antibody (1:100, CST, USA), anti-cytokeratin mouse mAb antibody (1:100, Abcam, USA) and anti-CD8 mouse antibody (1:100, Proteintech group, China) at 4 °C overnight. Cells were incubated with a secondary antibody, and DAB was used for color development. Cells were counterstained, neutral gum sealed and observed according to a standard immunohistochemical operation procedure. PBS was used as a negative control. The stained sections were scanned using Panoramic MIDI. Image J was used to count positively stained cells. Two senior pathologists independently confirmed the results.
Anti cytokeratin mouse mab antibody
Manufactured by Abcam
Sourced in United States
Anti-cytokeratin mouse mAb antibody is a monoclonal antibody that recognizes cytokeratins, a group of intermediate filament proteins found in the cytoplasm of epithelial cells. This antibody can be used for the detection and identification of epithelial cells in various applications.
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Lab products found in correlation
5 protocols using anti cytokeratin mouse mab antibody
1
Immunohistochemical Profiling of Primary GC Cells
2
Immunohistochemical Analysis of Immune Markers
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Fresh tissues or collected cells were xed, dehydrated and para n embedded. Para n sections were dewaxed and rehydrated using a routine protocol. The cells underwent antigen repair, neutralization of endogenous catalases, serum blocking, incubation with anti-CD137 rabbit mAb antibody (1:100, CST), anti-Foxp3 rabbit mAb antibody (1:100, CST), anti-cytokeratin mouse mAb antibody (1:100, Abcam) and anti-CD8 mouse antibody (1:100, Proteintech group) at 4°C overnight. Cells were incubated with a secondary antibody, and DAB was used for color development. Cells were counterstained, neutral gum sealed and observed according to a standard immunohistochemical operation procedure. PBS was used as a negative control. The stained sections were scanned using Panoramic MIDI. Image J was used to count positively stained cells. Two senior pathologists independently con rmed the results.
3
Multiplex Immunohistochemistry Protocol
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Fresh tissues or collected cells were xed, dehydrated and para n embedded. Para n sections were dewaxed and rehydrated according to a routine protocol, and then antigen repair, neutralization of endogenous catalases, serum blocking, incubation with anti-CD137 rabbit mAb antibody (1:100, CST), anti-Foxp3 rabbit mAb antibody (1:100, CST) , anti-Cytokeratin mouse mAb antibody (1:100, Abcam) and anti-CD8 mouse antibody (1:100, Proteintech group) respectively at 4°C overnight, incubation with a secondary antibody, DAB color development, counterstaining, neutral gum sealing and observation were carried out step by step according to a standard immunohistochemical operation procedure. PBS was used as a negative control. The stained sections were scanned by Panoramic MIDI. Image J was used to count positive staining cells. The results were con rmed independently by two senior pathologist.
4
Immunohistochemical Analysis of GC Cells
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Fresh tissues for phenotypic assays or collected primary GC cells for functional assays to test separation purity were xed, dehydrated and para n embedded. Para n sections were dewaxed and rehydrated using a routine protocol [22] . The cells underwent antigen repair, neutralization of endogenous catalases, serum blocking, incubation with anti-CD137 rabbit mAb antibody (1:100, CST, USA), anti-Foxp3 rabbit mAb antibody (1:100, CST, USA), anti-cytokeratin mouse mAb antibody (1:100, Abcam, USA) and anti-CD8 mouse antibody (1:100, Proteintech group, China) at 4°C overnight. Cells were incubated with a secondary antibody, and DAB was used for color development. Cells were counterstained, neutral gum sealed and observed according to a standard immunohistochemical operation procedure. PBS was used as a negative control. The stained sections were scanned using Panoramic MIDI. Image J was used to count positively stained cells. Two senior pathologists independently con rmed the results.
5
Immunohistochemical Analysis of GC Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fresh tissues for phenotypic assays or collected primary GC cells for functional assays to test separation purity were xed, dehydrated and para n embedded. Para n sections were dewaxed and rehydrated using a routine protocol [22] . The cells underwent antigen repair, neutralization of endogenous catalases, serum blocking, incubation with anti-CD137 rabbit mAb antibody (1:100, CST, USA), anti-Foxp3 rabbit mAb antibody (1:100, CST, USA), anti-cytokeratin mouse mAb antibody (1:100, Abcam, USA) and anti-CD8 mouse antibody (1:100, Proteintech group, China) at 4°C overnight. Cells were incubated with a secondary antibody, and DAB was used for color development. Cells were counterstained, neutral gum sealed and observed according to a standard immunohistochemical operation procedure. PBS was used as a negative control. The stained sections were scanned using Panoramic MIDI. Image J was used to count positively stained cells. Two senior pathologists independently con rmed the results.
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