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Click it edu microplate assay kit

Manufactured by RiboBio
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Sourced in Japan, China

The Click-iT® EdU Microplate Assay Kit is a fluorescence-based product used to measure cell proliferation. It detects the incorporation of the thymidine analog EdU (5-ethynyl-2'-deoxyuridine) into newly synthesized DNA during cell division.

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2 protocols using click it edu microplate assay kit

1

Cell Proliferation, Wound Healing, and Invasion Assays

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Cell proliferation was assayed with CCK-8 (Dojin Laboratories, Tokyo, Japan) and Click-iT® EdU Microplate Assay Kit (Ribobio, Guangzhou, China), according to the manufacturer’s instructions. Wounds were generated with 10 μL pipette tips. Matrigel invasion assay was performed as described previously [62 (link)]. See supplementary material for details.
Sun Z., Ou C., Liu J., Chen C., Zhou Q., Yang S., Li G., Wang G., Song J., Li Z., Zhang Z., Yuan W, & Li X. (2018). RETRACTED ARTICLE: YAP1-induced MALAT1 promotes epithelial–mesenchymal transition and angiogenesis by sponging miR-126-5p in colorectal cancer. Oncogene, 38(14), 2627-2644.
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2

Cell Proliferation Quantification via EdU

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Cell proliferation was detected with Click-iT®EdU Microplate Assay Kit (C10310-3, Ribobio, Beijing, China). Cells were planted in 24 well plate (1×105 cells/well). EdU (50¼M, 300¼L) which is modified by fluorescence was added into wells and incubated in chamber for 3 hours as the protocol indicates. After EdU staining, cells were counterstained with DAPI. EdU-positive cells was imaged randomly by fluorescence microscope FSX100 (Olympas, Japan) and the ratio of positive ones was counted.
Dai Y., Wang L., Tang J., Cao P., Luo Z., Sun J., Kiflu A., Sai B., Zhang M., Wang F., Li G, & Xiang J. (2016). Activation of anaphase-promoting complex by p53 induces a state of dormancy in cancer cells against chemotherapeutic stress. Oncotarget, 7(18), 25478-25492.
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