Female 6–7 weeks old nu/nu immunodeficient mice were injected subcutaneously with 107 S462TY cells in 0.1 ml 30% Matrigel (BD Biosciences)/PBS. Treatment started when the average tumor size reached 100 mm3. Nifedipine (Santa Cruz) was prepared as a suspension in aqueous 0.5% hypromellose/0.2% Tween 80 and administrated by daily gavage at 50 mg/kg. Norcantharidin (Santa Cruz) was formulated in PBS and IP injected at 10 mg/kg/day. Tumors were measured with a caliper every tree days. Mice were sacrificed when tumor size reached 10% of body weight, tumors were isolated and weighted.
Nifedipine
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Nifedipine is a calcium channel blocker used as a laboratory reagent. It functions by inhibiting the movement of calcium ions through calcium channels, which is a key process in various cellular and physiological functions.
Automatically generated - may contain errors
2 protocols using nifedipine
1
Tumor Growth Inhibition in nu/nu Mice
2
Protein Hydrolysates and Receptor Modulators
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Protein hydrolysates (casein: n°82303; meat: n°82962; pea: n°96174), lactisole, 4aminomethylbenzoic acid (AMBA) and probenecid were obtained from Sigma (St. Louis, USA). NPS-2143, tetrodotoxin (TTX) and nifedipine were purchased from Santa Cruz Biotechnology (Dallas, USA), Alomone labs (Jerusalem, Israel) and Tocris Bioscience (Ellisville, USA), respectively. For NPS-2143 and lactisole, stock solutions were made in DMSO and further dilutions were made in Krebs-Ringer buffer resulting in a final concentration of 0.00125% DMSO.
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