Water (H2O), methanol (MeOH), dichloromethane (DCM), pentane (Pent), hexane, isopropyl alcohol (IPA), ethyl acetate (EtAc), acetone, toluene, butanol (HPLC grade), sulfuric acid (H2SO4), hydrochloric acid (HCl), ammonium hydroxide (NH4OH), potassium carbonate (K2CO3), tetrasodium EDTA (Na4EDTA), and ammonium formate (AmFor) (reagent grade) were purchased from Fisher Scientific (USA).
Nicotine base was purchased from Sigma Aldrich (USA). Native nicotine and nicotine-d4 salts were synthesized as described in Ref.[30 ]. Nicotelline and deuterated nicotelline-d8 were synthesized as previously reported[16 (link)]. Deuterated nitrosamines NNK-d4, NNN-d4, NAT-d4, and NAB-d4 and native nitrosamines NNK, NNN, NAT, and NAB were purchased from Toronto Research Chemicals (Canada). The native PAH QTM mix and chrysene-d12, were purchased from Supelco (USA) and SPEXCertiPrep (USA), respectively. Deuterated PAH were purchased from Chemservice (USA). Agilent Bond Elut 10 mL and 1 mg silica column were purchased from Agilent (USA).
Nicotine base was purchased from Sigma Aldrich (USA). Native nicotine and nicotine-d4 salts were synthesized as described in Ref.[30 ]. Nicotelline and deuterated nicotelline-d8 were synthesized as previously reported[16 (link)]. Deuterated nitrosamines NNK-d4, NNN-d4, NAT-d4, and NAB-d4 and native nitrosamines NNK, NNN, NAT, and NAB were purchased from Toronto Research Chemicals (Canada). The native PAH QTM mix and chrysene-d12, were purchased from Supelco (USA) and SPEXCertiPrep (USA), respectively. Deuterated PAH were purchased from Chemservice (USA). Agilent Bond Elut 10 mL and 1 mg silica column were purchased from Agilent (USA).